Evaluation of agave bagasse recalcitrance using AFEX™, autohydrolysis, and ionic liquid pretreatments

Jose A. Perez-Pimienta, Carlos A. Flores-Gómez, Héctor A. Ruiz, Noppadon Sathitsuksanoh, Venkatesh Balan, Leonardo da Costa Sousa, Bruce E. Dale, Seema Singh, Blake A. Simmons

Research output: Contribution to journalArticle

  • 16 Citations

Abstract

A comparative analysis of the response of agave bagasse (AGB) to pretreatment by ammonia fiber expansion (AFEX™), autohydrolysis (AH) and ionic liquid (IL) was performed using 2D nuclear magnetic resonance (NMR) spectroscopy, wet chemistry, enzymatic saccharification and mass balances. It has been found that AFEX pretreatment preserved all carbohydrates in the biomass, whereas AH removed 62.4% of xylan and IL extracted 25% of lignin into wash streams. Syringyl and guaiacyl lignin ratio of untreated AGB was 4.3, whereas for the pretreated biomass the ratios were 4.2, 5.0 and 4.7 for AFEX, AH and IL, respectively. Using NMR spectra, the intensity of β-aryl ether units in aliphatic, anomeric, and aromatic regions decreased in all three pretreated samples when compared to untreated biomass. Yields of glucose plus xylose in the major hydrolysate stream were 42.5, 39.7 and 26.9 kg per 100 kg of untreated AGB for AFEX, IL and AH, respectively.

LanguageEnglish (US)
Pages216-223
Number of pages8
JournalBioresource Technology
Volume211
DOIs
StatePublished - Jul 1 2016

Profile

Ionic Liquids
Bagasse
Ionic liquids
Biomass
Lignin
lignin
nuclear magnetic resonance
biomass
Xylans
Saccharification
Xylose
Carbohydrates
Ammonia
ether
Ether
Nuclear magnetic resonance spectroscopy
Glucose
carbohydrate
Ethers
mass balance

Keywords

  • AFEX
  • Agave bagasse
  • Autohydrolysis
  • Biomass pretreatment
  • Characterization
  • Comparison
  • Ionic liquid

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Perez-Pimienta, J. A., Flores-Gómez, C. A., Ruiz, H. A., Sathitsuksanoh, N., Balan, V., da Costa Sousa, L., ... Simmons, B. A. (2016). Evaluation of agave bagasse recalcitrance using AFEX™, autohydrolysis, and ionic liquid pretreatments. Bioresource Technology, 211, 216-223. DOI: 10.1016/j.biortech.2016.03.103

Evaluation of agave bagasse recalcitrance using AFEX™, autohydrolysis, and ionic liquid pretreatments. / Perez-Pimienta, Jose A.; Flores-Gómez, Carlos A.; Ruiz, Héctor A.; Sathitsuksanoh, Noppadon; Balan, Venkatesh; da Costa Sousa, Leonardo; Dale, Bruce E.; Singh, Seema; Simmons, Blake A.

In: Bioresource Technology, Vol. 211, 01.07.2016, p. 216-223.

Research output: Contribution to journalArticle

Perez-Pimienta, JA, Flores-Gómez, CA, Ruiz, HA, Sathitsuksanoh, N, Balan, V, da Costa Sousa, L, Dale, BE, Singh, S & Simmons, BA 2016, 'Evaluation of agave bagasse recalcitrance using AFEX™, autohydrolysis, and ionic liquid pretreatments' Bioresource Technology, vol. 211, pp. 216-223. DOI: 10.1016/j.biortech.2016.03.103
Perez-Pimienta JA, Flores-Gómez CA, Ruiz HA, Sathitsuksanoh N, Balan V, da Costa Sousa L et al. Evaluation of agave bagasse recalcitrance using AFEX™, autohydrolysis, and ionic liquid pretreatments. Bioresource Technology. 2016 Jul 1;211:216-223. Available from, DOI: 10.1016/j.biortech.2016.03.103
Perez-Pimienta, Jose A. ; Flores-Gómez, Carlos A. ; Ruiz, Héctor A. ; Sathitsuksanoh, Noppadon ; Balan, Venkatesh ; da Costa Sousa, Leonardo ; Dale, Bruce E. ; Singh, Seema ; Simmons, Blake A./ Evaluation of agave bagasse recalcitrance using AFEX™, autohydrolysis, and ionic liquid pretreatments. In: Bioresource Technology. 2016 ; Vol. 211. pp. 216-223
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