Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading

Patricia J. Slininger, Maureen A. Shea-Andersh, Stephanie R. Thompson, Bruce S. Dien, Cletus P. Kurtzman, Venkatesh Balan, Leonardo Da Costa Sousa, Nirmal Uppugundla, Bruce E. Dale, Michael A. Cotta

Research output: Contribution to journalArticle

  • 13 Citations

Abstract

Background: Lignocellulosic biomass is an abundant, renewable feedstock useful for the production of fuel-grade ethanol via the processing steps of pretreatment, enzyme hydrolysis, and microbial fermentation. Traditional industrial yeasts do not ferment xylose and are not able to grow, survive, or ferment in concentrated hydrolyzates that contain enough sugar to support economical ethanol recovery since they are laden with toxic byproducts generated during pretreatment. Results: Repetitive culturing in two types of concentrated hydrolyzates was applied along with ethanol-challenged xylose-fed continuous culture to force targeted evolution of the native pentose fermenting yeast Scheffersomyces (Pichia) stipitis strain NRRL Y-7124 maintained in the ARS Culture Collection, Peoria, IL. Isolates collected from various enriched populations were screened and ranked based on relative xylose uptake rate and ethanol yield. Ranking on hydrolyzates with and without nutritional supplementation was used to identify those isolates with best performance across diverse conditions. Conclusions: Robust S. stipitis strains adapted to perform very well in enzyme hydrolyzates of high solids loading ammonia fiber expansion-pretreated corn stover (18% weight per volume solids) and dilute sulfuric acid-pretreated switchgrass (20% w/v solids) were obtained. Improved features include reduced initial lag phase preceding growth, significantly enhanced fermentation rates, improved ethanol tolerance and yield, reduced diauxic lag during glucose-xylose transition, and ability to accumulate >40 g/L ethanol in

LanguageEnglish (US)
Article number60
JournalBiotechnology for Biofuels
Volume8
Issue number1
DOIs
StatePublished - Apr 17 2015

Profile

Biomass
ethanol
Xylose
Ethanol
Productivity
productivity
Acids
acid
biomass
Yeast
Fermentation
fermentation
yeast
Enzymes
Yeasts
enzyme
Panicum
Pentoses
Pichia
Poisons

Keywords

  • Adaptation
  • Biofuel
  • Fermentation
  • Lignocellulose
  • Pichia stipitis
  • Yeast

ASJC Scopus subject areas

  • Energy(all)
  • Management, Monitoring, Policy and Law
  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Renewable Energy, Sustainability and the Environment

Cite this

Slininger, P. J., Shea-Andersh, M. A., Thompson, S. R., Dien, B. S., Kurtzman, C. P., Balan, V., ... Cotta, M. A. (2015). Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading. Biotechnology for Biofuels, 8(1), [60]. DOI: 10.1186/s13068-015-0239-6

Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading. / Slininger, Patricia J.; Shea-Andersh, Maureen A.; Thompson, Stephanie R.; Dien, Bruce S.; Kurtzman, Cletus P.; Balan, Venkatesh; Da Costa Sousa, Leonardo; Uppugundla, Nirmal; Dale, Bruce E.; Cotta, Michael A.

In: Biotechnology for Biofuels, Vol. 8, No. 1, 60, 17.04.2015.

Research output: Contribution to journalArticle

Slininger, Patricia J. ; Shea-Andersh, Maureen A. ; Thompson, Stephanie R. ; Dien, Bruce S. ; Kurtzman, Cletus P. ; Balan, Venkatesh ; Da Costa Sousa, Leonardo ; Uppugundla, Nirmal ; Dale, Bruce E. ; Cotta, Michael A./ Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading. In: Biotechnology for Biofuels. 2015 ; Vol. 8, No. 1.
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abstract = "Background: Lignocellulosic biomass is an abundant, renewable feedstock useful for the production of fuel-grade ethanol via the processing steps of pretreatment, enzyme hydrolysis, and microbial fermentation. Traditional industrial yeasts do not ferment xylose and are not able to grow, survive, or ferment in concentrated hydrolyzates that contain enough sugar to support economical ethanol recovery since they are laden with toxic byproducts generated during pretreatment. Results: Repetitive culturing in two types of concentrated hydrolyzates was applied along with ethanol-challenged xylose-fed continuous culture to force targeted evolution of the native pentose fermenting yeast Scheffersomyces (Pichia) stipitis strain NRRL Y-7124 maintained in the ARS Culture Collection, Peoria, IL. Isolates collected from various enriched populations were screened and ranked based on relative xylose uptake rate and ethanol yield. Ranking on hydrolyzates with and without nutritional supplementation was used to identify those isolates with best performance across diverse conditions. Conclusions: Robust S. stipitis strains adapted to perform very well in enzyme hydrolyzates of high solids loading ammonia fiber expansion-pretreated corn stover (18{\%} weight per volume solids) and dilute sulfuric acid-pretreated switchgrass (20{\%} w/v solids) were obtained. Improved features include reduced initial lag phase preceding growth, significantly enhanced fermentation rates, improved ethanol tolerance and yield, reduced diauxic lag during glucose-xylose transition, and ability to accumulate >40 g/L ethanol in",
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AU - Dien,Bruce S.

AU - Kurtzman,Cletus P.

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AU - Cotta,Michael A.

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