Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading

Patricia J. Slininger, Maureen A. Shea-Andersh, Stephanie R. Thompson, Bruce S. Dien, Cletus P. Kurtzman, Venkatesh Balan, Leonardo Da Costa Sousa, Nirmal Uppugundla, Bruce E. Dale, Michael A. Cotta

    Research output: Contribution to journalArticle

    • 9 Citations

    Abstract

    Background: Lignocellulosic biomass is an abundant, renewable feedstock useful for the production of fuel-grade ethanol via the processing steps of pretreatment, enzyme hydrolysis, and microbial fermentation. Traditional industrial yeasts do not ferment xylose and are not able to grow, survive, or ferment in concentrated hydrolyzates that contain enough sugar to support economical ethanol recovery since they are laden with toxic byproducts generated during pretreatment. Results: Repetitive culturing in two types of concentrated hydrolyzates was applied along with ethanol-challenged xylose-fed continuous culture to force targeted evolution of the native pentose fermenting yeast Scheffersomyces (Pichia) stipitis strain NRRL Y-7124 maintained in the ARS Culture Collection, Peoria, IL. Isolates collected from various enriched populations were screened and ranked based on relative xylose uptake rate and ethanol yield. Ranking on hydrolyzates with and without nutritional supplementation was used to identify those isolates with best performance across diverse conditions. Conclusions: Robust S. stipitis strains adapted to perform very well in enzyme hydrolyzates of high solids loading ammonia fiber expansion-pretreated corn stover (18% weight per volume solids) and dilute sulfuric acid-pretreated switchgrass (20% w/v solids) were obtained. Improved features include reduced initial lag phase preceding growth, significantly enhanced fermentation rates, improved ethanol tolerance and yield, reduced diauxic lag during glucose-xylose transition, and ability to accumulate >40 g/L ethanol in

    Original languageEnglish (US)
    Article number60
    JournalBiotechnology for Biofuels
    Volume8
    Issue number1
    DOIs
    StatePublished - Apr 17 2015

    Profile

    Biomass
    Ethanol
    Acids
    ethanol
    Xylose
    Fermentation
    Yeasts
    Enzymes
    Yeast
    fermentation
    yeast
    enzyme
    biomass
    Pentoses
    Pichia
    Poisons
    Ammonia
    Zea mays
    Hydrolysis
    Carbohydrates

    Keywords

    • Adaptation
    • Biofuel
    • Fermentation
    • Lignocellulose
    • Pichia stipitis
    • Yeast

    ASJC Scopus subject areas

    • Energy(all)
    • Management, Monitoring, Policy and Law
    • Biotechnology
    • Applied Microbiology and Biotechnology
    • Renewable Energy, Sustainability and the Environment

    Cite this

    Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading. / Slininger, Patricia J.; Shea-Andersh, Maureen A.; Thompson, Stephanie R.; Dien, Bruce S.; Kurtzman, Cletus P.; Balan, Venkatesh; Da Costa Sousa, Leonardo; Uppugundla, Nirmal; Dale, Bruce E.; Cotta, Michael A.

    In: Biotechnology for Biofuels, Vol. 8, No. 1, 60, 17.04.2015.

    Research output: Contribution to journalArticle

    Slininger, PJ, Shea-Andersh, MA, Thompson, SR, Dien, BS, Kurtzman, CP, Balan, V, Da Costa Sousa, L, Uppugundla, N, Dale, BE & Cotta, MA 2015, 'Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading' Biotechnology for Biofuels, vol 8, no. 1, 60. DOI: 10.1186/s13068-015-0239-6

    Slininger, Patricia J.; Shea-Andersh, Maureen A.; Thompson, Stephanie R.; Dien, Bruce S.; Kurtzman, Cletus P.; Balan, Venkatesh; Da Costa Sousa, Leonardo; Uppugundla, Nirmal; Dale, Bruce E.; Cotta, Michael A. / Evolved strains of Scheffersomyces stipitis achieving high ethanol productivity on acid- and base-pretreated biomass hydrolyzate at high solids loading.

    In: Biotechnology for Biofuels, Vol. 8, No. 1, 60, 17.04.2015.

    Research output: Contribution to journalArticle

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    abstract = "Background: Lignocellulosic biomass is an abundant, renewable feedstock useful for the production of fuel-grade ethanol via the processing steps of pretreatment, enzyme hydrolysis, and microbial fermentation. Traditional industrial yeasts do not ferment xylose and are not able to grow, survive, or ferment in concentrated hydrolyzates that contain enough sugar to support economical ethanol recovery since they are laden with toxic byproducts generated during pretreatment. Results: Repetitive culturing in two types of concentrated hydrolyzates was applied along with ethanol-challenged xylose-fed continuous culture to force targeted evolution of the native pentose fermenting yeast Scheffersomyces (Pichia) stipitis strain NRRL Y-7124 maintained in the ARS Culture Collection, Peoria, IL. Isolates collected from various enriched populations were screened and ranked based on relative xylose uptake rate and ethanol yield. Ranking on hydrolyzates with and without nutritional supplementation was used to identify those isolates with best performance across diverse conditions. Conclusions: Robust S. stipitis strains adapted to perform very well in enzyme hydrolyzates of high solids loading ammonia fiber expansion-pretreated corn stover (18% weight per volume solids) and dilute sulfuric acid-pretreated switchgrass (20% w/v solids) were obtained. Improved features include reduced initial lag phase preceding growth, significantly enhanced fermentation rates, improved ethanol tolerance and yield, reduced diauxic lag during glucose-xylose transition, and ability to accumulate >40 g/L ethanol in",
    keywords = "Adaptation, Biofuel, Fermentation, Lignocellulose, Pichia stipitis, Yeast",
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    AU - Slininger,Patricia J.

    AU - Shea-Andersh,Maureen A.

    AU - Thompson,Stephanie R.

    AU - Dien,Bruce S.

    AU - Kurtzman,Cletus P.

    AU - Balan,Venkatesh

    AU - Da Costa Sousa,Leonardo

    AU - Uppugundla,Nirmal

    AU - Dale,Bruce E.

    AU - Cotta,Michael A.

    PY - 2015/4/17

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    N2 - Background: Lignocellulosic biomass is an abundant, renewable feedstock useful for the production of fuel-grade ethanol via the processing steps of pretreatment, enzyme hydrolysis, and microbial fermentation. Traditional industrial yeasts do not ferment xylose and are not able to grow, survive, or ferment in concentrated hydrolyzates that contain enough sugar to support economical ethanol recovery since they are laden with toxic byproducts generated during pretreatment. Results: Repetitive culturing in two types of concentrated hydrolyzates was applied along with ethanol-challenged xylose-fed continuous culture to force targeted evolution of the native pentose fermenting yeast Scheffersomyces (Pichia) stipitis strain NRRL Y-7124 maintained in the ARS Culture Collection, Peoria, IL. Isolates collected from various enriched populations were screened and ranked based on relative xylose uptake rate and ethanol yield. Ranking on hydrolyzates with and without nutritional supplementation was used to identify those isolates with best performance across diverse conditions. Conclusions: Robust S. stipitis strains adapted to perform very well in enzyme hydrolyzates of high solids loading ammonia fiber expansion-pretreated corn stover (18% weight per volume solids) and dilute sulfuric acid-pretreated switchgrass (20% w/v solids) were obtained. Improved features include reduced initial lag phase preceding growth, significantly enhanced fermentation rates, improved ethanol tolerance and yield, reduced diauxic lag during glucose-xylose transition, and ability to accumulate >40 g/L ethanol in

    AB - Background: Lignocellulosic biomass is an abundant, renewable feedstock useful for the production of fuel-grade ethanol via the processing steps of pretreatment, enzyme hydrolysis, and microbial fermentation. Traditional industrial yeasts do not ferment xylose and are not able to grow, survive, or ferment in concentrated hydrolyzates that contain enough sugar to support economical ethanol recovery since they are laden with toxic byproducts generated during pretreatment. Results: Repetitive culturing in two types of concentrated hydrolyzates was applied along with ethanol-challenged xylose-fed continuous culture to force targeted evolution of the native pentose fermenting yeast Scheffersomyces (Pichia) stipitis strain NRRL Y-7124 maintained in the ARS Culture Collection, Peoria, IL. Isolates collected from various enriched populations were screened and ranked based on relative xylose uptake rate and ethanol yield. Ranking on hydrolyzates with and without nutritional supplementation was used to identify those isolates with best performance across diverse conditions. Conclusions: Robust S. stipitis strains adapted to perform very well in enzyme hydrolyzates of high solids loading ammonia fiber expansion-pretreated corn stover (18% weight per volume solids) and dilute sulfuric acid-pretreated switchgrass (20% w/v solids) were obtained. Improved features include reduced initial lag phase preceding growth, significantly enhanced fermentation rates, improved ethanol tolerance and yield, reduced diauxic lag during glucose-xylose transition, and ability to accumulate >40 g/L ethanol in

    KW - Adaptation

    KW - Biofuel

    KW - Fermentation

    KW - Lignocellulose

    KW - Pichia stipitis

    KW - Yeast

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