ExtractabilityStem cell MR detectability in animal models by modification of the inhalation gas

Uwe Himmelreich, Ralph Weber, Pedro Ramos-Cabrer, Susanne Wegener, Korinna Kandal, Eric M. Shapiro, Alan P. Koretsky, Mathias Hoehn

Research output: Research - peer-reviewArticle

  • 25 Citations

Abstract

In vivo monitoring of cells labeled with paramagnetic iron oxide particles by magnetic resonance imaging (MRI) is complicated by intrinsic contrast of blood vessels. Distinction between T 2* effects caused by blood vessels from those caused by labeled cells was so far only possible after carefully following the location of hypointense regions through subsequent slices of T 2*-weighted 3-D MRI datasets, a procedure that is time consuming and not always reliable in the case of smaller blood vessels. Here, we demonstrate that the modification of the inhalation gas mixture from the routinely used composition 35% O 2 and 65% N 2O to a mixture containing 95% O 2 and 5% CO 2 results in a contrast suppression of most small blood vessels and reduces the intrinsic T 2* effect of large vessels dramatically in an animal model. This change in protocol of physiological conditions was well tolerated by all studied animals, even over prolonged experimental times. The changed inhalation gas mixture thus provides a more reliable identification method for small clusters of iron oxide labeled cells in vivo.

LanguageEnglish (US)
Pages104-109
Number of pages6
JournalMolecular Imaging
Volume4
Issue number2
StatePublished - Apr 2005
Externally publishedYes

Profile

animal models
blood vessels
respiration
cells
gases
Blood vessels
Animals
Gases
Inhalation
Blood Vessels
Animal Models
iron oxides
gas mixtures
magnetic resonance
Magnetic resonance
Iron oxides
Gas mixtures
Imaging techniques
Magnetic Resonance Imaging
ferric oxide

Keywords

  • Animal model
  • BOLD effect
  • Cell labeling strategies
  • MRI contrast
  • Stem cells

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Himmelreich, U., Weber, R., Ramos-Cabrer, P., Wegener, S., Kandal, K., Shapiro, E. M., ... Hoehn, M. (2005). ExtractabilityStem cell MR detectability in animal models by modification of the inhalation gas. Molecular Imaging, 4(2), 104-109.

ExtractabilityStem cell MR detectability in animal models by modification of the inhalation gas. / Himmelreich, Uwe; Weber, Ralph; Ramos-Cabrer, Pedro; Wegener, Susanne; Kandal, Korinna; Shapiro, Eric M.; Koretsky, Alan P.; Hoehn, Mathias.

In: Molecular Imaging, Vol. 4, No. 2, 04.2005, p. 104-109.

Research output: Research - peer-reviewArticle

Himmelreich, U, Weber, R, Ramos-Cabrer, P, Wegener, S, Kandal, K, Shapiro, EM, Koretsky, AP & Hoehn, M 2005, 'ExtractabilityStem cell MR detectability in animal models by modification of the inhalation gas' Molecular Imaging, vol 4, no. 2, pp. 104-109.
Himmelreich U, Weber R, Ramos-Cabrer P, Wegener S, Kandal K, Shapiro EM et al. ExtractabilityStem cell MR detectability in animal models by modification of the inhalation gas. Molecular Imaging. 2005 Apr;4(2):104-109.
Himmelreich, Uwe ; Weber, Ralph ; Ramos-Cabrer, Pedro ; Wegener, Susanne ; Kandal, Korinna ; Shapiro, Eric M. ; Koretsky, Alan P. ; Hoehn, Mathias. / ExtractabilityStem cell MR detectability in animal models by modification of the inhalation gas. In: Molecular Imaging. 2005 ; Vol. 4, No. 2. pp. 104-109
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