Fed-batch hydrolysate addition and cell separation by settling in high cell density lignocellulosic ethanol fermentations on AFEX™ corn stover in the Rapid Bioconversion with Integrated recycling Technology process

Cory Sarks, Mingjie Jin, Venkatesh Balan, Bruce E. Dale

    Research output: Research - peer-reviewArticle

    Abstract

    The Rapid Bioconversion with Integrated recycling Technology (RaBIT) process uses enzyme and yeast recycling to improve cellulosic ethanol production economics. The previous versions of the RaBIT process exhibited decreased xylose consumption using cell recycle for a variety of different micro-organisms. Process changes were tested in an attempt to eliminate the xylose consumption decrease. Three different RaBIT process changes were evaluated in this work including (1) shortening the fermentation time, (2) fed-batch hydrolysate addition, and (3) selective cell recycling using a settling method. Shorting the RaBIT fermentation process to 11 h and introducing fed-batch hydrolysate addition eliminated any xylose consumption decrease over ten fermentation cycles; otherwise, decreased xylose consumption was apparent by the third cell recycle event. However, partial removal of yeast cells during recycle was not economical when compared to recycling all yeast cells.

    LanguageEnglish (US)
    Pages1-12
    Number of pages12
    JournalJournal of Industrial Microbiology and Biotechnology
    DOIs
    StateAccepted/In press - May 23 2017

    Profile

    Cell Separation
    Recycling
    Fermentation
    Zea mays
    Ethanol
    Cell Count
    Technology
    Xylose
    Yeasts
    Economics
    Enzymes

    Keywords

    • AFEX
    • Cellulosic ethanol
    • RaBIT
    • Recycling
    • Saccharomyces cerevisiae

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology

    Cite this

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    title = "Fed-batch hydrolysate addition and cell separation by settling in high cell density lignocellulosic ethanol fermentations on AFEX™ corn stover in the Rapid Bioconversion with Integrated recycling Technology process",
    abstract = "The Rapid Bioconversion with Integrated recycling Technology (RaBIT) process uses enzyme and yeast recycling to improve cellulosic ethanol production economics. The previous versions of the RaBIT process exhibited decreased xylose consumption using cell recycle for a variety of different micro-organisms. Process changes were tested in an attempt to eliminate the xylose consumption decrease. Three different RaBIT process changes were evaluated in this work including (1) shortening the fermentation time, (2) fed-batch hydrolysate addition, and (3) selective cell recycling using a settling method. Shorting the RaBIT fermentation process to 11 h and introducing fed-batch hydrolysate addition eliminated any xylose consumption decrease over ten fermentation cycles; otherwise, decreased xylose consumption was apparent by the third cell recycle event. However, partial removal of yeast cells during recycle was not economical when compared to recycling all yeast cells.",
    keywords = "AFEX, Cellulosic ethanol, RaBIT, Recycling, Saccharomyces cerevisiae",
    author = "Cory Sarks and Mingjie Jin and Venkatesh Balan and Dale, {Bruce E.}",
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    AU - Jin,Mingjie

    AU - Balan,Venkatesh

    AU - Dale,Bruce E.

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    KW - Recycling

    KW - Saccharomyces cerevisiae

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