Functional bioelectronic interfaces on electrolessly deposited gold for bioelectronic applications

Brian L. Hassler, Neeraj Kohli, Lavanya Parthasarathy, Robert Ofoli, Ilsoon Lee, R. Mark Worden

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Bioelectronic interfaces that establish electrical communication between redox enzymes and electrodes have potential applications as biosensors, biocatalytic reactors, and biological fuel cells. This paper describes the fabrication of functional, bioelectronic interfaces suitable for both soluble and membrane-bound enzymes on electrolessly deposited gold films. The suitability of the bioelectronic interfaces for soluble redox enzymes was evaluated using secondary alcohol dehydrogenase (sADH). A multilayered interface was assembled that enabled electron transfer between the electrode and sADH's cofactor (NADPH). The enzyme was then bound by affinity interaction with the cofactor. Membrane-bound redox enzymes must often be embedded in a bilayer lipid membrane (BLM) to express their activities, so a tethered BLM (tBLM) was assembled on the gold film. The bottom leaflet of the tBLM was formed using a mixture of 2-dipalmitoyl-sn-glycero-3-phosphothioethanol (DPTE) and 2-dioleoyl-sn-glycero-3-phosphate (DOPC), and the top leaflet was formed by DOPC liposome fusion. The suitability of the tBLM for incorporating membrane proteins was evaluated using the ion-channel protein valinomycin. Cyclic voltammetry, electrochemical impedance spectroscopy, and fluorescence recovery after pattern photobleaching (FRAPP) were used to characterize the resulting bioelectronic interfaces. These interfaces were then assembled into patterned microarrays using microcontact printing to pattern the surface prior to gold deposition.

Original languageEnglish (US)
Title of host publicationAIChE Annual Meeting, Conference Proceedings
Pages14237
Number of pages1
StatePublished - 2005
Externally publishedYes
Event05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, United States

Other

Other05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
CountryUnited States
CityCincinnati, OH
Period10/30/0511/4/05

Profile

Enzymes
Gold
Membranes
Lipid bilayers
Proteins
Electrodes
Biological fuel cells
Photobleaching
Liposomes
Microarrays
Electrochemical impedance spectroscopy
Biosensors
Cyclic voltammetry
Printing
Phosphates
Alcohols
Fusion reactions
Fluorescence
Fabrication
Recovery

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Hassler, B. L., Kohli, N., Parthasarathy, L., Ofoli, R., Lee, I., & Worden, R. M. (2005). Functional bioelectronic interfaces on electrolessly deposited gold for bioelectronic applications. In AIChE Annual Meeting, Conference Proceedings (pp. 14237)

Functional bioelectronic interfaces on electrolessly deposited gold for bioelectronic applications. / Hassler, Brian L.; Kohli, Neeraj; Parthasarathy, Lavanya; Ofoli, Robert; Lee, Ilsoon; Worden, R. Mark.

AIChE Annual Meeting, Conference Proceedings. 2005. p. 14237.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hassler, BL, Kohli, N, Parthasarathy, L, Ofoli, R, Lee, I & Worden, RM 2005, Functional bioelectronic interfaces on electrolessly deposited gold for bioelectronic applications. in AIChE Annual Meeting, Conference Proceedings. pp. 14237, 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Cincinnati, OH, United States, 30-4 November.
Hassler BL, Kohli N, Parthasarathy L, Ofoli R, Lee I, Worden RM. Functional bioelectronic interfaces on electrolessly deposited gold for bioelectronic applications. In AIChE Annual Meeting, Conference Proceedings. 2005. p. 14237.

Hassler, Brian L.; Kohli, Neeraj; Parthasarathy, Lavanya; Ofoli, Robert; Lee, Ilsoon; Worden, R. Mark / Functional bioelectronic interfaces on electrolessly deposited gold for bioelectronic applications.

AIChE Annual Meeting, Conference Proceedings. 2005. p. 14237.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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