Fungal fermentation on anaerobic digestate for lipid-based biofuel production

Yuan Zhong, Zhiguo Liu, Christine Isaguirre, Yan Liu, Wei Liao

    Research output: Research - peer-reviewArticle

    Abstract

    Background: Anaerobic digestate is the effluent from anaerobic digestion of organic wastes. It contains a significant amount of nutrients and lignocellulosic materials, even though anaerobic digestion consumed a large portion of organic matters in the wastes. Utilizing the nutrients and lignocellulosic materials in the digestate is critical to significantly improve efficiency of anaerobic digestion technology and generate value-added chemical and fuel products from the organic wastes. Therefore, this study focused on developing an integrated process that uses biogas energy to power fungal fermentation and converts remaining carbon sources, nutrients, and water in the digestate into biofuel precursor-lipid. Results: The process contains two unit operations of anaerobic digestion and digestate utilization. The digestate utilization includes alkali treatment of the mixture feed of solid and liquid digestates, enzymatic hydrolysis for mono-sugar release, overliming detoxification, and fungal fermentation for lipid accumulation. The experimental results conclude that 5 h and 30°C were the preferred conditions for the overliming detoxification regarding lipid accumulation of the following fungal cultivation. The repeated-batch fungal fermentation enhanced lipid accumulation, which led to a final lipid concentration of 3.16 g/L on the digestate with 10% dry matter. The mass and energy balance analysis further indicates that the digestate had enough water for the process uses and the biogas energy was able to balance the needs of individual unit operations. Conclusions: A fresh-water-free and energy-positive process of lipid production from anaerobic digestate was achieved by integrating anaerobic digestion and fungal fermentation. The integration addresses the issues that both biofuel industry and waste management encounter - high water and energy demand of biofuel precursor production and few digestate utilization approaches of organic waste treatment.

    LanguageEnglish (US)
    Article number253
    JournalBiotechnology for Biofuels
    Volume9
    Issue number1
    DOIs
    StatePublished - Nov 21 2016

    Profile

    biofuel
    fermentation
    lipid
    Biofuels
    Fermentation
    Lipids
    anaerobic digestion
    Anaerobic digestion
    Digestion
    Water
    nutrient
    water
    organic waste
    Nutrients
    Food
    detoxification
    biogas
    energy
    material
    Detoxification

    Keywords

    • Anaerobic digestion
    • Biodiesel
    • Energy-positive
    • Fresh-water-free
    • Fungal fermentation
    • Lipid
    • Repeated-batch culture

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology
    • Renewable Energy, Sustainability and the Environment
    • Energy(all)
    • Management, Monitoring, Policy and Law

    Cite this

    Fungal fermentation on anaerobic digestate for lipid-based biofuel production. / Zhong, Yuan; Liu, Zhiguo; Isaguirre, Christine; Liu, Yan; Liao, Wei.

    In: Biotechnology for Biofuels, Vol. 9, No. 1, 253, 21.11.2016.

    Research output: Research - peer-reviewArticle

    @article{ffe470afc96747beb57da91ed2224a20,
    title = "Fungal fermentation on anaerobic digestate for lipid-based biofuel production",
    abstract = "Background: Anaerobic digestate is the effluent from anaerobic digestion of organic wastes. It contains a significant amount of nutrients and lignocellulosic materials, even though anaerobic digestion consumed a large portion of organic matters in the wastes. Utilizing the nutrients and lignocellulosic materials in the digestate is critical to significantly improve efficiency of anaerobic digestion technology and generate value-added chemical and fuel products from the organic wastes. Therefore, this study focused on developing an integrated process that uses biogas energy to power fungal fermentation and converts remaining carbon sources, nutrients, and water in the digestate into biofuel precursor-lipid. Results: The process contains two unit operations of anaerobic digestion and digestate utilization. The digestate utilization includes alkali treatment of the mixture feed of solid and liquid digestates, enzymatic hydrolysis for mono-sugar release, overliming detoxification, and fungal fermentation for lipid accumulation. The experimental results conclude that 5 h and 30°C were the preferred conditions for the overliming detoxification regarding lipid accumulation of the following fungal cultivation. The repeated-batch fungal fermentation enhanced lipid accumulation, which led to a final lipid concentration of 3.16 g/L on the digestate with 10% dry matter. The mass and energy balance analysis further indicates that the digestate had enough water for the process uses and the biogas energy was able to balance the needs of individual unit operations. Conclusions: A fresh-water-free and energy-positive process of lipid production from anaerobic digestate was achieved by integrating anaerobic digestion and fungal fermentation. The integration addresses the issues that both biofuel industry and waste management encounter - high water and energy demand of biofuel precursor production and few digestate utilization approaches of organic waste treatment.",
    keywords = "Anaerobic digestion, Biodiesel, Energy-positive, Fresh-water-free, Fungal fermentation, Lipid, Repeated-batch culture",
    author = "Yuan Zhong and Zhiguo Liu and Christine Isaguirre and Yan Liu and Wei Liao",
    year = "2016",
    month = "11",
    doi = "10.1186/s13068-016-0654-3",
    volume = "9",
    journal = "Biotechnology for Biofuels",
    issn = "1754-6834",
    publisher = "BioMed Central",
    number = "1",

    }

    TY - JOUR

    T1 - Fungal fermentation on anaerobic digestate for lipid-based biofuel production

    AU - Zhong,Yuan

    AU - Liu,Zhiguo

    AU - Isaguirre,Christine

    AU - Liu,Yan

    AU - Liao,Wei

    PY - 2016/11/21

    Y1 - 2016/11/21

    N2 - Background: Anaerobic digestate is the effluent from anaerobic digestion of organic wastes. It contains a significant amount of nutrients and lignocellulosic materials, even though anaerobic digestion consumed a large portion of organic matters in the wastes. Utilizing the nutrients and lignocellulosic materials in the digestate is critical to significantly improve efficiency of anaerobic digestion technology and generate value-added chemical and fuel products from the organic wastes. Therefore, this study focused on developing an integrated process that uses biogas energy to power fungal fermentation and converts remaining carbon sources, nutrients, and water in the digestate into biofuel precursor-lipid. Results: The process contains two unit operations of anaerobic digestion and digestate utilization. The digestate utilization includes alkali treatment of the mixture feed of solid and liquid digestates, enzymatic hydrolysis for mono-sugar release, overliming detoxification, and fungal fermentation for lipid accumulation. The experimental results conclude that 5 h and 30°C were the preferred conditions for the overliming detoxification regarding lipid accumulation of the following fungal cultivation. The repeated-batch fungal fermentation enhanced lipid accumulation, which led to a final lipid concentration of 3.16 g/L on the digestate with 10% dry matter. The mass and energy balance analysis further indicates that the digestate had enough water for the process uses and the biogas energy was able to balance the needs of individual unit operations. Conclusions: A fresh-water-free and energy-positive process of lipid production from anaerobic digestate was achieved by integrating anaerobic digestion and fungal fermentation. The integration addresses the issues that both biofuel industry and waste management encounter - high water and energy demand of biofuel precursor production and few digestate utilization approaches of organic waste treatment.

    AB - Background: Anaerobic digestate is the effluent from anaerobic digestion of organic wastes. It contains a significant amount of nutrients and lignocellulosic materials, even though anaerobic digestion consumed a large portion of organic matters in the wastes. Utilizing the nutrients and lignocellulosic materials in the digestate is critical to significantly improve efficiency of anaerobic digestion technology and generate value-added chemical and fuel products from the organic wastes. Therefore, this study focused on developing an integrated process that uses biogas energy to power fungal fermentation and converts remaining carbon sources, nutrients, and water in the digestate into biofuel precursor-lipid. Results: The process contains two unit operations of anaerobic digestion and digestate utilization. The digestate utilization includes alkali treatment of the mixture feed of solid and liquid digestates, enzymatic hydrolysis for mono-sugar release, overliming detoxification, and fungal fermentation for lipid accumulation. The experimental results conclude that 5 h and 30°C were the preferred conditions for the overliming detoxification regarding lipid accumulation of the following fungal cultivation. The repeated-batch fungal fermentation enhanced lipid accumulation, which led to a final lipid concentration of 3.16 g/L on the digestate with 10% dry matter. The mass and energy balance analysis further indicates that the digestate had enough water for the process uses and the biogas energy was able to balance the needs of individual unit operations. Conclusions: A fresh-water-free and energy-positive process of lipid production from anaerobic digestate was achieved by integrating anaerobic digestion and fungal fermentation. The integration addresses the issues that both biofuel industry and waste management encounter - high water and energy demand of biofuel precursor production and few digestate utilization approaches of organic waste treatment.

    KW - Anaerobic digestion

    KW - Biodiesel

    KW - Energy-positive

    KW - Fresh-water-free

    KW - Fungal fermentation

    KW - Lipid

    KW - Repeated-batch culture

    UR - http://www.scopus.com/inward/record.url?scp=84995791075&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84995791075&partnerID=8YFLogxK

    U2 - 10.1186/s13068-016-0654-3

    DO - 10.1186/s13068-016-0654-3

    M3 - Article

    VL - 9

    JO - Biotechnology for Biofuels

    T2 - Biotechnology for Biofuels

    JF - Biotechnology for Biofuels

    SN - 1754-6834

    IS - 1

    M1 - 253

    ER -