Fungal fermentation on anaerobic digestate for lipid-based biofuel production

Yuan Zhong, Zhiguo Liu, Christine Isaguirre, Yan Liu, Wei Liao

Research output: Contribution to journalArticle

Abstract

Background: Anaerobic digestate is the effluent from anaerobic digestion of organic wastes. It contains a significant amount of nutrients and lignocellulosic materials, even though anaerobic digestion consumed a large portion of organic matters in the wastes. Utilizing the nutrients and lignocellulosic materials in the digestate is critical to significantly improve efficiency of anaerobic digestion technology and generate value-added chemical and fuel products from the organic wastes. Therefore, this study focused on developing an integrated process that uses biogas energy to power fungal fermentation and converts remaining carbon sources, nutrients, and water in the digestate into biofuel precursor-lipid. Results: The process contains two unit operations of anaerobic digestion and digestate utilization. The digestate utilization includes alkali treatment of the mixture feed of solid and liquid digestates, enzymatic hydrolysis for mono-sugar release, overliming detoxification, and fungal fermentation for lipid accumulation. The experimental results conclude that 5 h and 30°C were the preferred conditions for the overliming detoxification regarding lipid accumulation of the following fungal cultivation. The repeated-batch fungal fermentation enhanced lipid accumulation, which led to a final lipid concentration of 3.16 g/L on the digestate with 10% dry matter. The mass and energy balance analysis further indicates that the digestate had enough water for the process uses and the biogas energy was able to balance the needs of individual unit operations. Conclusions: A fresh-water-free and energy-positive process of lipid production from anaerobic digestate was achieved by integrating anaerobic digestion and fungal fermentation. The integration addresses the issues that both biofuel industry and waste management encounter - high water and energy demand of biofuel precursor production and few digestate utilization approaches of organic waste treatment.

LanguageEnglish (US)
Article number253
JournalBiotechnology for Biofuels
Volume9
Issue number1
DOIs
StatePublished - Nov 21 2016

Profile

Biofuels
Anaerobic digestion
biofuel
Fermentation
Lipids
fermentation
lipid
Digestion
Nutrients
Detoxification
Biogas
detoxification
biogas
Food
Water
nutrient
Waste Products
Waste Management
Enzymatic hydrolysis
Waste treatment

Keywords

  • Anaerobic digestion
  • Biodiesel
  • Energy-positive
  • Fresh-water-free
  • Fungal fermentation
  • Lipid
  • Repeated-batch culture

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Renewable Energy, Sustainability and the Environment
  • Energy(all)
  • Management, Monitoring, Policy and Law

Cite this

Fungal fermentation on anaerobic digestate for lipid-based biofuel production. / Zhong, Yuan; Liu, Zhiguo; Isaguirre, Christine; Liu, Yan; Liao, Wei.

In: Biotechnology for Biofuels, Vol. 9, No. 1, 253, 21.11.2016.

Research output: Contribution to journalArticle

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AB - Background: Anaerobic digestate is the effluent from anaerobic digestion of organic wastes. It contains a significant amount of nutrients and lignocellulosic materials, even though anaerobic digestion consumed a large portion of organic matters in the wastes. Utilizing the nutrients and lignocellulosic materials in the digestate is critical to significantly improve efficiency of anaerobic digestion technology and generate value-added chemical and fuel products from the organic wastes. Therefore, this study focused on developing an integrated process that uses biogas energy to power fungal fermentation and converts remaining carbon sources, nutrients, and water in the digestate into biofuel precursor-lipid. Results: The process contains two unit operations of anaerobic digestion and digestate utilization. The digestate utilization includes alkali treatment of the mixture feed of solid and liquid digestates, enzymatic hydrolysis for mono-sugar release, overliming detoxification, and fungal fermentation for lipid accumulation. The experimental results conclude that 5 h and 30°C were the preferred conditions for the overliming detoxification regarding lipid accumulation of the following fungal cultivation. The repeated-batch fungal fermentation enhanced lipid accumulation, which led to a final lipid concentration of 3.16 g/L on the digestate with 10% dry matter. The mass and energy balance analysis further indicates that the digestate had enough water for the process uses and the biogas energy was able to balance the needs of individual unit operations. Conclusions: A fresh-water-free and energy-positive process of lipid production from anaerobic digestate was achieved by integrating anaerobic digestion and fungal fermentation. The integration addresses the issues that both biofuel industry and waste management encounter - high water and energy demand of biofuel precursor production and few digestate utilization approaches of organic waste treatment.

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