Growth promotive conditions for enhanced eritadenine production during submerged cultivation of Lentinus edodes

Josefine Enman, David Hodge, Kris Arvid Berglund, Ulrika Rova

    Research output: Research - peer-reviewArticle

    • 2 Citations

    Abstract

    Background: Mycelium of the medicinal mushroom shiitake, Lentinus edodes, is a potential source for production of the blood cholesterol reducing compound eritadenine. To increase the mycelial biomass and in turn the production of eritadenine, a potential growth promoting substance in the form of a water extract of distillers dried grains with solubles (DDGS) was added to the culture media. Results: The hot water extract of DDGS was shown to considerably increase the growth of shiitake mycelia in bioreactor cultivations; the mycelial yield was 2-3 times higher than in the control, and the highest final biomass concentration obtained was 3.4 g L -1. Further, by using shake flask cultures as inoculums the bioreactor cultivation time could be reduced by 1 week for some of the experiments. The highest final titer of eritadenine in the present study was 25.1 mg L -1, which was about 2 times higher than in the control, and was also obtained when a water extract of DDGS was added to the culture medium. Conclusion: It was demonstrated that a water extract of DDGS promoted the growth of shiitake mycelia in bioreactor cultivations, along with enhanced eritadenine production.

    LanguageEnglish (US)
    Pages903-907
    Number of pages5
    JournalJournal of Chemical Technology and Biotechnology
    Volume87
    Issue number7
    DOIs
    StatePublished - Jul 2012

    Profile

    Shiitake Mushrooms
    Water
    Growth
    eritadenine
    bioreactor
    water
    Mycelium
    Bioreactors
    biomass
    Biomass
    Culture Media
    mushroom
    blood
    experiment
    hot water
    Growth Substances
    Batch Cell Culture Techniques
    Cholesterol
    Blood
    Experiments

    Keywords

    • Eritadenine
    • Growth promotion
    • Lentinus edodes
    • Submerged cultivation

    ASJC Scopus subject areas

    • Inorganic Chemistry
    • Waste Management and Disposal
    • Pollution
    • Organic Chemistry
    • Renewable Energy, Sustainability and the Environment
    • Biotechnology
    • Chemical Engineering(all)
    • Fuel Technology

    Cite this

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    abstract = "Background: Mycelium of the medicinal mushroom shiitake, Lentinus edodes, is a potential source for production of the blood cholesterol reducing compound eritadenine. To increase the mycelial biomass and in turn the production of eritadenine, a potential growth promoting substance in the form of a water extract of distillers dried grains with solubles (DDGS) was added to the culture media. Results: The hot water extract of DDGS was shown to considerably increase the growth of shiitake mycelia in bioreactor cultivations; the mycelial yield was 2-3 times higher than in the control, and the highest final biomass concentration obtained was 3.4 g L -1. Further, by using shake flask cultures as inoculums the bioreactor cultivation time could be reduced by 1 week for some of the experiments. The highest final titer of eritadenine in the present study was 25.1 mg L -1, which was about 2 times higher than in the control, and was also obtained when a water extract of DDGS was added to the culture medium. Conclusion: It was demonstrated that a water extract of DDGS promoted the growth of shiitake mycelia in bioreactor cultivations, along with enhanced eritadenine production.",
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    author = "Josefine Enman and David Hodge and Berglund, {Kris Arvid} and Ulrika Rova",
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    T1 - Growth promotive conditions for enhanced eritadenine production during submerged cultivation of Lentinus edodes

    AU - Enman,Josefine

    AU - Hodge,David

    AU - Berglund,Kris Arvid

    AU - Rova,Ulrika

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    N2 - Background: Mycelium of the medicinal mushroom shiitake, Lentinus edodes, is a potential source for production of the blood cholesterol reducing compound eritadenine. To increase the mycelial biomass and in turn the production of eritadenine, a potential growth promoting substance in the form of a water extract of distillers dried grains with solubles (DDGS) was added to the culture media. Results: The hot water extract of DDGS was shown to considerably increase the growth of shiitake mycelia in bioreactor cultivations; the mycelial yield was 2-3 times higher than in the control, and the highest final biomass concentration obtained was 3.4 g L -1. Further, by using shake flask cultures as inoculums the bioreactor cultivation time could be reduced by 1 week for some of the experiments. The highest final titer of eritadenine in the present study was 25.1 mg L -1, which was about 2 times higher than in the control, and was also obtained when a water extract of DDGS was added to the culture medium. Conclusion: It was demonstrated that a water extract of DDGS promoted the growth of shiitake mycelia in bioreactor cultivations, along with enhanced eritadenine production.

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