Growth promotive conditions for enhanced eritadenine production during submerged cultivation of Lentinus edodes

Josefine Enman, David Hodge, Kris Arvid Berglund, Ulrika Rova

Research output: Contribution to journalArticle

  • 2 Citations

Abstract

Background: Mycelium of the medicinal mushroom shiitake, Lentinus edodes, is a potential source for production of the blood cholesterol reducing compound eritadenine. To increase the mycelial biomass and in turn the production of eritadenine, a potential growth promoting substance in the form of a water extract of distillers dried grains with solubles (DDGS) was added to the culture media. Results: The hot water extract of DDGS was shown to considerably increase the growth of shiitake mycelia in bioreactor cultivations; the mycelial yield was 2-3 times higher than in the control, and the highest final biomass concentration obtained was 3.4 g L -1. Further, by using shake flask cultures as inoculums the bioreactor cultivation time could be reduced by 1 week for some of the experiments. The highest final titer of eritadenine in the present study was 25.1 mg L -1, which was about 2 times higher than in the control, and was also obtained when a water extract of DDGS was added to the culture medium. Conclusion: It was demonstrated that a water extract of DDGS promoted the growth of shiitake mycelia in bioreactor cultivations, along with enhanced eritadenine production.

LanguageEnglish (US)
Pages903-907
Number of pages5
JournalJournal of Chemical Technology and Biotechnology
Volume87
Issue number7
DOIs
StatePublished - Jul 2012

Profile

Shiitake Mushrooms
bioreactor
Mycelium
Bioreactors
Water
Growth
Biomass
Culture Media
mushroom
biomass
Growth Substances
water
Batch Cell Culture Techniques
Cholesterol
blood
Blood
eritadenine
experiment
Experiments

Keywords

  • Eritadenine
  • Growth promotion
  • Lentinus edodes
  • Submerged cultivation

ASJC Scopus subject areas

  • Inorganic Chemistry
  • Waste Management and Disposal
  • Pollution
  • Organic Chemistry
  • Renewable Energy, Sustainability and the Environment
  • Biotechnology
  • Chemical Engineering(all)
  • Fuel Technology

Cite this

Growth promotive conditions for enhanced eritadenine production during submerged cultivation of Lentinus edodes. / Enman, Josefine; Hodge, David; Berglund, Kris Arvid; Rova, Ulrika.

In: Journal of Chemical Technology and Biotechnology, Vol. 87, No. 7, 07.2012, p. 903-907.

Research output: Contribution to journalArticle

@article{bd259171f035402ca38225759a006fb1,
title = "Growth promotive conditions for enhanced eritadenine production during submerged cultivation of Lentinus edodes",
abstract = "Background: Mycelium of the medicinal mushroom shiitake, Lentinus edodes, is a potential source for production of the blood cholesterol reducing compound eritadenine. To increase the mycelial biomass and in turn the production of eritadenine, a potential growth promoting substance in the form of a water extract of distillers dried grains with solubles (DDGS) was added to the culture media. Results: The hot water extract of DDGS was shown to considerably increase the growth of shiitake mycelia in bioreactor cultivations; the mycelial yield was 2-3 times higher than in the control, and the highest final biomass concentration obtained was 3.4 g L -1. Further, by using shake flask cultures as inoculums the bioreactor cultivation time could be reduced by 1 week for some of the experiments. The highest final titer of eritadenine in the present study was 25.1 mg L -1, which was about 2 times higher than in the control, and was also obtained when a water extract of DDGS was added to the culture medium. Conclusion: It was demonstrated that a water extract of DDGS promoted the growth of shiitake mycelia in bioreactor cultivations, along with enhanced eritadenine production.",
keywords = "Eritadenine, Growth promotion, Lentinus edodes, Submerged cultivation",
author = "Josefine Enman and David Hodge and Berglund, {Kris Arvid} and Ulrika Rova",
year = "2012",
month = "7",
doi = "10.1002/jctb.3697",
language = "English (US)",
volume = "87",
pages = "903--907",
journal = "Journal of Chemical Technology and Biotechnology",
issn = "0268-2575",
publisher = "John Wiley and Sons Ltd",
number = "7",

}

TY - JOUR

T1 - Growth promotive conditions for enhanced eritadenine production during submerged cultivation of Lentinus edodes

AU - Enman,Josefine

AU - Hodge,David

AU - Berglund,Kris Arvid

AU - Rova,Ulrika

PY - 2012/7

Y1 - 2012/7

N2 - Background: Mycelium of the medicinal mushroom shiitake, Lentinus edodes, is a potential source for production of the blood cholesterol reducing compound eritadenine. To increase the mycelial biomass and in turn the production of eritadenine, a potential growth promoting substance in the form of a water extract of distillers dried grains with solubles (DDGS) was added to the culture media. Results: The hot water extract of DDGS was shown to considerably increase the growth of shiitake mycelia in bioreactor cultivations; the mycelial yield was 2-3 times higher than in the control, and the highest final biomass concentration obtained was 3.4 g L -1. Further, by using shake flask cultures as inoculums the bioreactor cultivation time could be reduced by 1 week for some of the experiments. The highest final titer of eritadenine in the present study was 25.1 mg L -1, which was about 2 times higher than in the control, and was also obtained when a water extract of DDGS was added to the culture medium. Conclusion: It was demonstrated that a water extract of DDGS promoted the growth of shiitake mycelia in bioreactor cultivations, along with enhanced eritadenine production.

AB - Background: Mycelium of the medicinal mushroom shiitake, Lentinus edodes, is a potential source for production of the blood cholesterol reducing compound eritadenine. To increase the mycelial biomass and in turn the production of eritadenine, a potential growth promoting substance in the form of a water extract of distillers dried grains with solubles (DDGS) was added to the culture media. Results: The hot water extract of DDGS was shown to considerably increase the growth of shiitake mycelia in bioreactor cultivations; the mycelial yield was 2-3 times higher than in the control, and the highest final biomass concentration obtained was 3.4 g L -1. Further, by using shake flask cultures as inoculums the bioreactor cultivation time could be reduced by 1 week for some of the experiments. The highest final titer of eritadenine in the present study was 25.1 mg L -1, which was about 2 times higher than in the control, and was also obtained when a water extract of DDGS was added to the culture medium. Conclusion: It was demonstrated that a water extract of DDGS promoted the growth of shiitake mycelia in bioreactor cultivations, along with enhanced eritadenine production.

KW - Eritadenine

KW - Growth promotion

KW - Lentinus edodes

KW - Submerged cultivation

UR - http://www.scopus.com/inward/record.url?scp=84862224967&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862224967&partnerID=8YFLogxK

U2 - 10.1002/jctb.3697

DO - 10.1002/jctb.3697

M3 - Article

VL - 87

SP - 903

EP - 907

JO - Journal of Chemical Technology and Biotechnology

T2 - Journal of Chemical Technology and Biotechnology

JF - Journal of Chemical Technology and Biotechnology

SN - 0268-2575

IS - 7

ER -