Growth promotive conditions for enhanced eritadenine production during submerged cultivation of Lentinus edodes

Josefine Enman, David Hodge, Kris Arvid Berglund, Ulrika Rova

    Research output: Contribution to journalArticle

    • 2 Citations

    Abstract

    Background: Mycelium of the medicinal mushroom shiitake, Lentinus edodes, is a potential source for production of the blood cholesterol reducing compound eritadenine. To increase the mycelial biomass and in turn the production of eritadenine, a potential growth promoting substance in the form of a water extract of distillers dried grains with solubles (DDGS) was added to the culture media. Results: The hot water extract of DDGS was shown to considerably increase the growth of shiitake mycelia in bioreactor cultivations; the mycelial yield was 2-3 times higher than in the control, and the highest final biomass concentration obtained was 3.4 g L -1. Further, by using shake flask cultures as inoculums the bioreactor cultivation time could be reduced by 1 week for some of the experiments. The highest final titer of eritadenine in the present study was 25.1 mg L -1, which was about 2 times higher than in the control, and was also obtained when a water extract of DDGS was added to the culture medium. Conclusion: It was demonstrated that a water extract of DDGS promoted the growth of shiitake mycelia in bioreactor cultivations, along with enhanced eritadenine production.

    Original languageEnglish (US)
    Pages (from-to)903-907
    Number of pages5
    JournalJournal of Chemical Technology and Biotechnology
    Volume87
    Issue number7
    DOIs
    StatePublished - Jul 2012

    Profile

    Shiitake Mushrooms
    Cereals
    Water
    water
    Erythrasma
    Glucagon-Like Peptides
    Mycelium
    Bioreactors
    bioreactor
    Cephalothin
    Biomass
    Culture Media
    biomass
    Panthera
    Blood Flow Velocity
    Growth Substances
    Cholesterol
    Blood
    Experiments
    mushroom

    Keywords

    • Eritadenine
    • Growth promotion
    • Lentinus edodes
    • Submerged cultivation

    ASJC Scopus subject areas

    • Inorganic Chemistry
    • Waste Management and Disposal
    • Pollution
    • Organic Chemistry
    • Renewable Energy, Sustainability and the Environment
    • Biotechnology
    • Chemical Engineering(all)
    • Fuel Technology

    Cite this

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    title = "Growth promotive conditions for enhanced eritadenine production during submerged cultivation of Lentinus edodes",
    abstract = "Background: Mycelium of the medicinal mushroom shiitake, Lentinus edodes, is a potential source for production of the blood cholesterol reducing compound eritadenine. To increase the mycelial biomass and in turn the production of eritadenine, a potential growth promoting substance in the form of a water extract of distillers dried grains with solubles (DDGS) was added to the culture media. Results: The hot water extract of DDGS was shown to considerably increase the growth of shiitake mycelia in bioreactor cultivations; the mycelial yield was 2-3 times higher than in the control, and the highest final biomass concentration obtained was 3.4 g L -1. Further, by using shake flask cultures as inoculums the bioreactor cultivation time could be reduced by 1 week for some of the experiments. The highest final titer of eritadenine in the present study was 25.1 mg L -1, which was about 2 times higher than in the control, and was also obtained when a water extract of DDGS was added to the culture medium. Conclusion: It was demonstrated that a water extract of DDGS promoted the growth of shiitake mycelia in bioreactor cultivations, along with enhanced eritadenine production.",
    keywords = "Eritadenine, Growth promotion, Lentinus edodes, Submerged cultivation",
    author = "Josefine Enman and David Hodge and Berglund, {Kris Arvid} and Ulrika Rova",
    year = "2012",
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    doi = "10.1002/jctb.3697",
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    pages = "903--907",
    journal = "Journal of Chemical Technology and Biotechnology",
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    T1 - Growth promotive conditions for enhanced eritadenine production during submerged cultivation of Lentinus edodes

    AU - Enman,Josefine

    AU - Hodge,David

    AU - Berglund,Kris Arvid

    AU - Rova,Ulrika

    PY - 2012/7

    Y1 - 2012/7

    N2 - Background: Mycelium of the medicinal mushroom shiitake, Lentinus edodes, is a potential source for production of the blood cholesterol reducing compound eritadenine. To increase the mycelial biomass and in turn the production of eritadenine, a potential growth promoting substance in the form of a water extract of distillers dried grains with solubles (DDGS) was added to the culture media. Results: The hot water extract of DDGS was shown to considerably increase the growth of shiitake mycelia in bioreactor cultivations; the mycelial yield was 2-3 times higher than in the control, and the highest final biomass concentration obtained was 3.4 g L -1. Further, by using shake flask cultures as inoculums the bioreactor cultivation time could be reduced by 1 week for some of the experiments. The highest final titer of eritadenine in the present study was 25.1 mg L -1, which was about 2 times higher than in the control, and was also obtained when a water extract of DDGS was added to the culture medium. Conclusion: It was demonstrated that a water extract of DDGS promoted the growth of shiitake mycelia in bioreactor cultivations, along with enhanced eritadenine production.

    AB - Background: Mycelium of the medicinal mushroom shiitake, Lentinus edodes, is a potential source for production of the blood cholesterol reducing compound eritadenine. To increase the mycelial biomass and in turn the production of eritadenine, a potential growth promoting substance in the form of a water extract of distillers dried grains with solubles (DDGS) was added to the culture media. Results: The hot water extract of DDGS was shown to considerably increase the growth of shiitake mycelia in bioreactor cultivations; the mycelial yield was 2-3 times higher than in the control, and the highest final biomass concentration obtained was 3.4 g L -1. Further, by using shake flask cultures as inoculums the bioreactor cultivation time could be reduced by 1 week for some of the experiments. The highest final titer of eritadenine in the present study was 25.1 mg L -1, which was about 2 times higher than in the control, and was also obtained when a water extract of DDGS was added to the culture medium. Conclusion: It was demonstrated that a water extract of DDGS promoted the growth of shiitake mycelia in bioreactor cultivations, along with enhanced eritadenine production.

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    KW - Growth promotion

    KW - Lentinus edodes

    KW - Submerged cultivation

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