Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cells

Kathleen A. Hinds, Jonathan M. Hill, Erik M. Shapiro, Mikko O. Laukkanen, Alfonso C. Silva, Christian A. Combs, Timothy R. Varney, Robert S. Balaban, Alan P. Koretsky, Cynthia E. Dunbar

Research output: Research - peer-reviewArticle

  • 333 Citations

Abstract

Tracking transplanted stem cells using magnetic resonance imaging (MRI) could offer biologic insight into homing and engraftment. Ultrasmall dextran-coated iron oxide particles have previously been developed for uptake into cells to allow MRI tracking. We describe a new application of much larger, micron-scale, iron oxide magnetic particles with enhanced MR susceptibility, which enables detection of single cells at resolutions that can be achieved in vivo. In addition, these larger particles possess a fluorophore for histologic confirmation of cell distribution. We demonstrate highly efficient, nontoxic, endosomal uptake of these particles into hematopoietic CD34+ cells and mesenchymal stem cells documented by confocal and electron microscopy. Labeled cells retain biologic activity with preservation of colony-forming ability and differentiation capacity. MRI studies could detect labeled CD34+ cells and mesenchymal stem cells (MSCs) at single cell resolution. This appears to be a promising tool for serial noninvasive monitoring of in vivo cell homing and localization using MRI.

LanguageEnglish (US)
Pages867-872
Number of pages6
JournalBlood
Volume102
Issue number3
DOIs
StatePublished - Aug 1 2003
Externally publishedYes

Profile

Magnetic resonance
Stem cells
Labeling
Imaging techniques
Stem Cells
Magnetic Resonance Imaging
ferric oxide
Fluorophores
Confocal microscopy
Dextrans
Electron microscopy
Monitoring
Mesenchymal Stromal Cells
Confocal Microscopy
Electron Microscopy

ASJC Scopus subject areas

  • Hematology

Cite this

Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cells. / Hinds, Kathleen A.; Hill, Jonathan M.; Shapiro, Erik M.; Laukkanen, Mikko O.; Silva, Alfonso C.; Combs, Christian A.; Varney, Timothy R.; Balaban, Robert S.; Koretsky, Alan P.; Dunbar, Cynthia E.

In: Blood, Vol. 102, No. 3, 01.08.2003, p. 867-872.

Research output: Research - peer-reviewArticle

Hinds, KA, Hill, JM, Shapiro, EM, Laukkanen, MO, Silva, AC, Combs, CA, Varney, TR, Balaban, RS, Koretsky, AP & Dunbar, CE 2003, 'Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cells' Blood, vol 102, no. 3, pp. 867-872. DOI: 10.1182/blood-2002-12-3669
Hinds, Kathleen A. ; Hill, Jonathan M. ; Shapiro, Erik M. ; Laukkanen, Mikko O. ; Silva, Alfonso C. ; Combs, Christian A. ; Varney, Timothy R. ; Balaban, Robert S. ; Koretsky, Alan P. ; Dunbar, Cynthia E./ Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cells. In: Blood. 2003 ; Vol. 102, No. 3. pp. 867-872
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