Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cells

Kathleen A. Hinds, Jonathan M. Hill, Erik M. Shapiro, Mikko O. Laukkanen, Alfonso C. Silva, Christian A. Combs, Timothy R. Varney, Robert S. Balaban, Alan P. Koretsky, Cynthia E. Dunbar

Research output: Contribution to journalArticle

  • 332 Citations

Abstract

Tracking transplanted stem cells using magnetic resonance imaging (MRI) could offer biologic insight into homing and engraftment. Ultrasmall dextran-coated iron oxide particles have previously been developed for uptake into cells to allow MRI tracking. We describe a new application of much larger, micron-scale, iron oxide magnetic particles with enhanced MR susceptibility, which enables detection of single cells at resolutions that can be achieved in vivo. In addition, these larger particles possess a fluorophore for histologic confirmation of cell distribution. We demonstrate highly efficient, nontoxic, endosomal uptake of these particles into hematopoietic CD34+ cells and mesenchymal stem cells documented by confocal and electron microscopy. Labeled cells retain biologic activity with preservation of colony-forming ability and differentiation capacity. MRI studies could detect labeled CD34+ cells and mesenchymal stem cells (MSCs) at single cell resolution. This appears to be a promising tool for serial noninvasive monitoring of in vivo cell homing and localization using MRI.

Original languageEnglish (US)
Pages (from-to)867-872
Number of pages6
JournalBlood
Volume102
Issue number3
DOIs
StatePublished - Aug 1 2003
Externally publishedYes

Profile

Adenofibroma
Guanine Nucleotides
Stem Cells
Magnetic Resonance Imaging
Status Epilepticus
Mesenchymal Stromal Cells
Oxides
Iron
Antimony Potassium Tartrate
Ear Ossicles
Fluorophores
Mastication
Electron microscopy
Dextrans
Confocal Microscopy
Electron Microscopy

ASJC Scopus subject areas

  • Hematology

Cite this

Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cells. / Hinds, Kathleen A.; Hill, Jonathan M.; Shapiro, Erik M.; Laukkanen, Mikko O.; Silva, Alfonso C.; Combs, Christian A.; Varney, Timothy R.; Balaban, Robert S.; Koretsky, Alan P.; Dunbar, Cynthia E.

In: Blood, Vol. 102, No. 3, 01.08.2003, p. 867-872.

Research output: Contribution to journalArticle

Hinds, KA, Hill, JM, Shapiro, EM, Laukkanen, MO, Silva, AC, Combs, CA, Varney, TR, Balaban, RS, Koretsky, AP & Dunbar, CE 2003, 'Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cells' Blood, vol 102, no. 3, pp. 867-872. DOI: 10.1182/blood-2002-12-3669

Hinds, Kathleen A.; Hill, Jonathan M.; Shapiro, Erik M.; Laukkanen, Mikko O.; Silva, Alfonso C.; Combs, Christian A.; Varney, Timothy R.; Balaban, Robert S.; Koretsky, Alan P.; Dunbar, Cynthia E. / Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cells.

In: Blood, Vol. 102, No. 3, 01.08.2003, p. 867-872.

Research output: Contribution to journalArticle

@article{66a1dc1e21fa4dbb852177fec5fd3e27,
title = "Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cells",
abstract = "Tracking transplanted stem cells using magnetic resonance imaging (MRI) could offer biologic insight into homing and engraftment. Ultrasmall dextran-coated iron oxide particles have previously been developed for uptake into cells to allow MRI tracking. We describe a new application of much larger, micron-scale, iron oxide magnetic particles with enhanced MR susceptibility, which enables detection of single cells at resolutions that can be achieved in vivo. In addition, these larger particles possess a fluorophore for histologic confirmation of cell distribution. We demonstrate highly efficient, nontoxic, endosomal uptake of these particles into hematopoietic CD34+ cells and mesenchymal stem cells documented by confocal and electron microscopy. Labeled cells retain biologic activity with preservation of colony-forming ability and differentiation capacity. MRI studies could detect labeled CD34+ cells and mesenchymal stem cells (MSCs) at single cell resolution. This appears to be a promising tool for serial noninvasive monitoring of in vivo cell homing and localization using MRI.",
author = "Hinds, {Kathleen A.} and Hill, {Jonathan M.} and Shapiro, {Erik M.} and Laukkanen, {Mikko O.} and Silva, {Alfonso C.} and Combs, {Christian A.} and Varney, {Timothy R.} and Balaban, {Robert S.} and Koretsky, {Alan P.} and Dunbar, {Cynthia E.}",
year = "2003",
month = "8",
doi = "10.1182/blood-2002-12-3669",
volume = "102",
pages = "867--872",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "3",

}

TY - JOUR

T1 - Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cells

AU - Hinds,Kathleen A.

AU - Hill,Jonathan M.

AU - Shapiro,Erik M.

AU - Laukkanen,Mikko O.

AU - Silva,Alfonso C.

AU - Combs,Christian A.

AU - Varney,Timothy R.

AU - Balaban,Robert S.

AU - Koretsky,Alan P.

AU - Dunbar,Cynthia E.

PY - 2003/8/1

Y1 - 2003/8/1

N2 - Tracking transplanted stem cells using magnetic resonance imaging (MRI) could offer biologic insight into homing and engraftment. Ultrasmall dextran-coated iron oxide particles have previously been developed for uptake into cells to allow MRI tracking. We describe a new application of much larger, micron-scale, iron oxide magnetic particles with enhanced MR susceptibility, which enables detection of single cells at resolutions that can be achieved in vivo. In addition, these larger particles possess a fluorophore for histologic confirmation of cell distribution. We demonstrate highly efficient, nontoxic, endosomal uptake of these particles into hematopoietic CD34+ cells and mesenchymal stem cells documented by confocal and electron microscopy. Labeled cells retain biologic activity with preservation of colony-forming ability and differentiation capacity. MRI studies could detect labeled CD34+ cells and mesenchymal stem cells (MSCs) at single cell resolution. This appears to be a promising tool for serial noninvasive monitoring of in vivo cell homing and localization using MRI.

AB - Tracking transplanted stem cells using magnetic resonance imaging (MRI) could offer biologic insight into homing and engraftment. Ultrasmall dextran-coated iron oxide particles have previously been developed for uptake into cells to allow MRI tracking. We describe a new application of much larger, micron-scale, iron oxide magnetic particles with enhanced MR susceptibility, which enables detection of single cells at resolutions that can be achieved in vivo. In addition, these larger particles possess a fluorophore for histologic confirmation of cell distribution. We demonstrate highly efficient, nontoxic, endosomal uptake of these particles into hematopoietic CD34+ cells and mesenchymal stem cells documented by confocal and electron microscopy. Labeled cells retain biologic activity with preservation of colony-forming ability and differentiation capacity. MRI studies could detect labeled CD34+ cells and mesenchymal stem cells (MSCs) at single cell resolution. This appears to be a promising tool for serial noninvasive monitoring of in vivo cell homing and localization using MRI.

UR - http://www.scopus.com/inward/record.url?scp=0043245963&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0043245963&partnerID=8YFLogxK

U2 - 10.1182/blood-2002-12-3669

DO - 10.1182/blood-2002-12-3669

M3 - Article

VL - 102

SP - 867

EP - 872

JO - Blood

T2 - Blood

JF - Blood

SN - 0006-4971

IS - 3

ER -