Identification of a novel benzimidazole that inhibits bacterial biofilm formation in a broad-spectrum manner

Karthik Sambanthamoorthy, Ankush A. Gokhale, Weiwei Lao, Vijay Parashar, Matthew B. Neiditch, Martin F. Semmelhack, Ilsoon Lee, Christopher M. Waters

    Research output: Research - peer-reviewArticle

    • 40 Citations

    Abstract

    Bacterial biofilm formation causes significant industrial economic loss and high morbidity and mortality in medical settings. Biofilms are defined as multicellular communities of bacteria encased in a matrix of protective extracellular polymers. Because biofilms have a high tolerance for treatment with antimicrobials, protect bacteria from immune defense, and resist clearance with standard sanitation protocols, it is critical to develop new approaches to prevent biofilm formation. Here, a novel benzimidazole molecule, named antibiofilm compound 1 (ABC-1), identified in a small-molecule screen, was found to prevent bacterial biofilm formation in multiple Gram-negative and Gram-positive bacterial pathogens, including Pseudomonas aeruginosa and Staphylococcus aureus, on a variety of different surface types. Importantly, ABC-1 itself does not inhibit the growth of bacteria, and it is effective at nanomolar concentrations. Also, coating a polystyrene surface with ABC-1 reduces biofilm formation. These data suggest ABC-1 is a new chemical scaffold for the development of antibiofilm compounds.

    LanguageEnglish (US)
    Pages4369-4378
    Number of pages10
    JournalAntimicrobial Agents and Chemotherapy
    Volume55
    Issue number9
    DOIs
    StatePublished - Sep 2011

    Profile

    Biofilms
    benzimidazole
    Bacteria
    Sanitation
    Polystyrenes
    Pseudomonas aeruginosa
    Extracellular Matrix
    Staphylococcus aureus
    Polymers
    Economics
    Morbidity
    Mortality
    Growth

    ASJC Scopus subject areas

    • Pharmacology (medical)
    • Pharmacology
    • Infectious Diseases

    Cite this

    Sambanthamoorthy, K., Gokhale, A. A., Lao, W., Parashar, V., Neiditch, M. B., Semmelhack, M. F., ... Waters, C. M. (2011). Identification of a novel benzimidazole that inhibits bacterial biofilm formation in a broad-spectrum manner. Antimicrobial Agents and Chemotherapy, 55(9), 4369-4378. DOI: 10.1128/AAC.00583-11

    Identification of a novel benzimidazole that inhibits bacterial biofilm formation in a broad-spectrum manner. / Sambanthamoorthy, Karthik; Gokhale, Ankush A.; Lao, Weiwei; Parashar, Vijay; Neiditch, Matthew B.; Semmelhack, Martin F.; Lee, Ilsoon; Waters, Christopher M.

    In: Antimicrobial Agents and Chemotherapy, Vol. 55, No. 9, 09.2011, p. 4369-4378.

    Research output: Research - peer-reviewArticle

    Sambanthamoorthy, K, Gokhale, AA, Lao, W, Parashar, V, Neiditch, MB, Semmelhack, MF, Lee, I & Waters, CM 2011, 'Identification of a novel benzimidazole that inhibits bacterial biofilm formation in a broad-spectrum manner' Antimicrobial Agents and Chemotherapy, vol 55, no. 9, pp. 4369-4378. DOI: 10.1128/AAC.00583-11
    Sambanthamoorthy K, Gokhale AA, Lao W, Parashar V, Neiditch MB, Semmelhack MF et al. Identification of a novel benzimidazole that inhibits bacterial biofilm formation in a broad-spectrum manner. Antimicrobial Agents and Chemotherapy. 2011 Sep;55(9):4369-4378. Available from, DOI: 10.1128/AAC.00583-11
    Sambanthamoorthy, Karthik ; Gokhale, Ankush A. ; Lao, Weiwei ; Parashar, Vijay ; Neiditch, Matthew B. ; Semmelhack, Martin F. ; Lee, Ilsoon ; Waters, Christopher M./ Identification of a novel benzimidazole that inhibits bacterial biofilm formation in a broad-spectrum manner. In: Antimicrobial Agents and Chemotherapy. 2011 ; Vol. 55, No. 9. pp. 4369-4378
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