Impact of oxygen on glucose oxidation kinetics in a redox polymer mediated glucose oxidase electrode

Harshal M. Bambhania, Deboleena Chakraborty, Hao Wen, Scott Calabrese Barton

    Research output: Contribution to journalArticle

    Abstract

    The kinetics of glucose oxidation by glucose oxidase (GOx) were evaluated with mediation by two different osmium redox polymers and in the presence and absence of oxygen (O2). A one-dimensional model was designed to account for the presence of dissolved oxygen, which competes with the mediator for adsorption sites on GOx and electrons from glucose oxidation. Kinetic parameters were determined by fitting the experimental current density measurements for varying potential, glucose and O2 concentrations, accounting for competitive inhibition and competitive reaction. It was demonstrated that the presence of O2 does not change the turnover number, kcat, of the enzyme, indicative of a competitive inhibition mechanism. However, improved fitting of the model was observed when considered the competitive reaction of O2. Kinetic parameters specific to the mediator reaction were an order of magnitude higher than other homogeneous mediated systems, leading to high mediation efficiency. Bimolecular rate constants for the GOx-oxygen reaction were found to be an order of magnitude lower than that of the free solution, which can be attributed to the reduced activity of immobilized GOx.

    Original languageEnglish (US)
    Pages (from-to)H232-H240
    JournalJournal of the Electrochemical Society
    Volume164
    Issue number4
    DOIs
    StatePublished - 2017

    Profile

    Glucose oxidase
    glucose
    Glucose
    Oxidation
    Oxygen
    Avian Leukosis
    oxidase
    kinetics
    oxygen
    Enzyme kinetics
    Kinetic parameters
    Polymers
    Anthralin
    Oxidation-Reduction
    Kinetics
    oxidation
    mediation
    polymers
    Enzyme inhibition
    Osmium

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Renewable Energy, Sustainability and the Environment
    • Condensed Matter Physics
    • Surfaces, Coatings and Films
    • Materials Chemistry
    • Electrochemistry

    Cite this

    Impact of oxygen on glucose oxidation kinetics in a redox polymer mediated glucose oxidase electrode. / Bambhania, Harshal M.; Chakraborty, Deboleena; Wen, Hao; Barton, Scott Calabrese.

    In: Journal of the Electrochemical Society, Vol. 164, No. 4, 2017, p. H232-H240.

    Research output: Contribution to journalArticle

    Bambhania, Harshal M.; Chakraborty, Deboleena; Wen, Hao; Barton, Scott Calabrese / Impact of oxygen on glucose oxidation kinetics in a redox polymer mediated glucose oxidase electrode.

    In: Journal of the Electrochemical Society, Vol. 164, No. 4, 2017, p. H232-H240.

    Research output: Contribution to journalArticle

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