Impacts of delignification and hot water pretreatment on the water induced cell wall swelling behavior of grasses and its relation to cellulolytic enzyme hydrolysis and binding

Daniel L. Williams, David B. Hodge

Research output: Contribution to journalArticle

  • 23 Citations

Abstract

The relationships between biomass composition, water retention value (WRV), settling volume and enzymatic glucose yield and enzyme binding is investigated in this work by employing grasses pretreated with combinations of alkaline hydrogen peroxide (AHP) delignification and liquid hot water pretreatment that result in significant alterations of cell wall properties and subsequent enzymatic hydrolysis yields. Specifically, these cell wall treatments are performed on corn stover and switchgrass to generate material with a range of lignin (6-35 %) and xylan (2-28 %) contents as well as a range of other properties such as carboxylic acid content, water binding affinity and swellability. It was determined that WRV and settling volume are predictors of glucose yield (R2 = 0.900 and 0.895 respectively) over the range of materials and treatment conditions used. It was also observed that mild AHP delignification can result in threefold increases in the WRV. Dynamic vapor sorption isotherms demonstrated that AHP-delignified corn stover exhibited an increased affinity for water sorption from the vapor phase relative to untreated corn stover. These results indicate that these water properties may be useful proxies for biomass susceptibility to enzymatic deconstruction.

LanguageEnglish (US)
Pages221-235
Number of pages15
JournalCellulose
Volume21
Issue number1
DOIs
StatePublished - Feb 2014

Profile

Delignification
Swelling
Hydrolysis
Enzymes
Cells
Water
Hydrogen Peroxide
Hydrogen peroxide
Sorption
Biomass
Glucose
Xylans
Vapors
Enzymatic hydrolysis
Lignin
Steam
Carboxylic Acids
Water content
Isotherms
Carboxylic acids

Keywords

  • Cell wall swelling
  • Enzymatic hydrolysis
  • Pretreatment
  • Water retention value

ASJC Scopus subject areas

  • Polymers and Plastics

Cite this

Impacts of delignification and hot water pretreatment on the water induced cell wall swelling behavior of grasses and its relation to cellulolytic enzyme hydrolysis and binding. / Williams, Daniel L.; Hodge, David B.

In: Cellulose, Vol. 21, No. 1, 02.2014, p. 221-235.

Research output: Contribution to journalArticle

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