Impacts of delignification and hot water pretreatment on the water induced cell wall swelling behavior of grasses and its relation to cellulolytic enzyme hydrolysis and binding

Daniel L. Williams, David B. Hodge

    Research output: Contribution to journalArticle

    • 21 Citations

    Abstract

    The relationships between biomass composition, water retention value (WRV), settling volume and enzymatic glucose yield and enzyme binding is investigated in this work by employing grasses pretreated with combinations of alkaline hydrogen peroxide (AHP) delignification and liquid hot water pretreatment that result in significant alterations of cell wall properties and subsequent enzymatic hydrolysis yields. Specifically, these cell wall treatments are performed on corn stover and switchgrass to generate material with a range of lignin (6-35 %) and xylan (2-28 %) contents as well as a range of other properties such as carboxylic acid content, water binding affinity and swellability. It was determined that WRV and settling volume are predictors of glucose yield (R2 = 0.900 and 0.895 respectively) over the range of materials and treatment conditions used. It was also observed that mild AHP delignification can result in threefold increases in the WRV. Dynamic vapor sorption isotherms demonstrated that AHP-delignified corn stover exhibited an increased affinity for water sorption from the vapor phase relative to untreated corn stover. These results indicate that these water properties may be useful proxies for biomass susceptibility to enzymatic deconstruction.

    Original languageEnglish (US)
    Pages (from-to)221-235
    Number of pages15
    JournalCellulose
    Volume21
    Issue number1
    DOIs
    StatePublished - Feb 2014

    Profile

    Water
    Delignification
    Castration
    Hydrogen peroxide
    Cells
    Panthera
    Traffic Accidents
    Acetates
    Glucose
    Sorption
    Biomass
    Enzymes
    Vapors
    African horse sickness virus
    Almanacs
    Birth Certificates
    Spontaneous Fractures
    Enzymatic hydrolysis
    Water content
    Swelling

    Keywords

    • Cell wall swelling
    • Enzymatic hydrolysis
    • Pretreatment
    • Water retention value

    ASJC Scopus subject areas

    • Polymers and Plastics

    Cite this

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    AB - The relationships between biomass composition, water retention value (WRV), settling volume and enzymatic glucose yield and enzyme binding is investigated in this work by employing grasses pretreated with combinations of alkaline hydrogen peroxide (AHP) delignification and liquid hot water pretreatment that result in significant alterations of cell wall properties and subsequent enzymatic hydrolysis yields. Specifically, these cell wall treatments are performed on corn stover and switchgrass to generate material with a range of lignin (6-35 %) and xylan (2-28 %) contents as well as a range of other properties such as carboxylic acid content, water binding affinity and swellability. It was determined that WRV and settling volume are predictors of glucose yield (R2 = 0.900 and 0.895 respectively) over the range of materials and treatment conditions used. It was also observed that mild AHP delignification can result in threefold increases in the WRV. Dynamic vapor sorption isotherms demonstrated that AHP-delignified corn stover exhibited an increased affinity for water sorption from the vapor phase relative to untreated corn stover. These results indicate that these water properties may be useful proxies for biomass susceptibility to enzymatic deconstruction.

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    KW - Enzymatic hydrolysis

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    KW - Water retention value

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