Abstract
Cellulase represents a major cost for biochemical conversion of lignocellulosic biomass to biofuels. In the present study, in-house production of cellulases from Ammonia Fiber Expansion pretreated corn stover (AFEX-CS) using Trichoderma reesei Rut C-30 was investigated. Seed culture was conducted in a simple medium containing corn steep liquor (CSL) and AFEX-CS hydrolysate. The effects of fermentation medium components (CSL, AFEX-CS, Tween 80 and CaCO3) on cellulase production were investigated and a simple fermentation medium was developed containing 20 g L-1 AFEX-CS and 4.0 g L-1 CaCO3. A cellulase activity of 1.5 FPU per mL was produced in this medium. Addition of 0.5% (v/v) CSL in the fermentation medium further improved cellulase activity (1.9 FPU per mL). Enzymatic hydrolysis of AFEX-CS using in-house produced enzymes generated high sugar yields with high enzyme loading. Proteomics analyses indicated that EGI and CBHII were deficient in the in-house produced enzyme cocktail when compared to the optimal cocktail for hydrolyzing AFEX-CS.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 25960-25969 |
| Number of pages | 10 |
| Journal | RSC Advances |
| Volume | 3 |
| Issue number | 48 |
| DOIs | |
| State | Published - Dec 28 2013 |
Profile
ASJC Scopus subject areas
- Chemical Engineering(all)
- Chemistry(all)
Cite this
In-house cellulase production from AFEX™ pretreated corn stover using Trichoderma reesei RUT C-30. / Culbertson, Alan; Jin, Mingjie; Da Costa Sousa, Leonardo; Dale, Bruce E.; Balan, Venkatesh.
In: RSC Advances, Vol. 3, No. 48, 28.12.2013, p. 25960-25969.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In-house cellulase production from AFEX™ pretreated corn stover using Trichoderma reesei RUT C-30
AU - Culbertson,Alan
AU - Jin,Mingjie
AU - Da Costa Sousa,Leonardo
AU - Dale,Bruce E.
AU - Balan,Venkatesh
PY - 2013/12/28
Y1 - 2013/12/28
N2 - Cellulase represents a major cost for biochemical conversion of lignocellulosic biomass to biofuels. In the present study, in-house production of cellulases from Ammonia Fiber Expansion pretreated corn stover (AFEX-CS) using Trichoderma reesei Rut C-30 was investigated. Seed culture was conducted in a simple medium containing corn steep liquor (CSL) and AFEX-CS hydrolysate. The effects of fermentation medium components (CSL, AFEX-CS, Tween 80 and CaCO3) on cellulase production were investigated and a simple fermentation medium was developed containing 20 g L-1 AFEX-CS and 4.0 g L-1 CaCO3. A cellulase activity of 1.5 FPU per mL was produced in this medium. Addition of 0.5% (v/v) CSL in the fermentation medium further improved cellulase activity (1.9 FPU per mL). Enzymatic hydrolysis of AFEX-CS using in-house produced enzymes generated high sugar yields with high enzyme loading. Proteomics analyses indicated that EGI and CBHII were deficient in the in-house produced enzyme cocktail when compared to the optimal cocktail for hydrolyzing AFEX-CS.
AB - Cellulase represents a major cost for biochemical conversion of lignocellulosic biomass to biofuels. In the present study, in-house production of cellulases from Ammonia Fiber Expansion pretreated corn stover (AFEX-CS) using Trichoderma reesei Rut C-30 was investigated. Seed culture was conducted in a simple medium containing corn steep liquor (CSL) and AFEX-CS hydrolysate. The effects of fermentation medium components (CSL, AFEX-CS, Tween 80 and CaCO3) on cellulase production were investigated and a simple fermentation medium was developed containing 20 g L-1 AFEX-CS and 4.0 g L-1 CaCO3. A cellulase activity of 1.5 FPU per mL was produced in this medium. Addition of 0.5% (v/v) CSL in the fermentation medium further improved cellulase activity (1.9 FPU per mL). Enzymatic hydrolysis of AFEX-CS using in-house produced enzymes generated high sugar yields with high enzyme loading. Proteomics analyses indicated that EGI and CBHII were deficient in the in-house produced enzyme cocktail when compared to the optimal cocktail for hydrolyzing AFEX-CS.
UR - http://www.scopus.com/inward/record.url?scp=84887913109&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84887913109&partnerID=8YFLogxK
U2 - 10.1039/c3ra44847a
DO - 10.1039/c3ra44847a
M3 - Article
VL - 3
SP - 25960
EP - 25969
JO - RSC Advances
T2 - RSC Advances
JF - RSC Advances
SN - 2046-2069
IS - 48
ER -