In-house cellulase production from AFEX™ pretreated corn stover using Trichoderma reesei RUT C-30

Alan Culbertson, Mingjie Jin, Leonardo Da Costa Sousa, Bruce E. Dale, Venkatesh Balan

    Research output: Contribution to journalArticle

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    Abstract

    Cellulase represents a major cost for biochemical conversion of lignocellulosic biomass to biofuels. In the present study, in-house production of cellulases from Ammonia Fiber Expansion pretreated corn stover (AFEX-CS) using Trichoderma reesei Rut C-30 was investigated. Seed culture was conducted in a simple medium containing corn steep liquor (CSL) and AFEX-CS hydrolysate. The effects of fermentation medium components (CSL, AFEX-CS, Tween 80 and CaCO3) on cellulase production were investigated and a simple fermentation medium was developed containing 20 g L-1 AFEX-CS and 4.0 g L-1 CaCO3. A cellulase activity of 1.5 FPU per mL was produced in this medium. Addition of 0.5% (v/v) CSL in the fermentation medium further improved cellulase activity (1.9 FPU per mL). Enzymatic hydrolysis of AFEX-CS using in-house produced enzymes generated high sugar yields with high enzyme loading. Proteomics analyses indicated that EGI and CBHII were deficient in the in-house produced enzyme cocktail when compared to the optimal cocktail for hydrolyzing AFEX-CS.

    Original languageEnglish (US)
    Pages (from-to)25960-25969
    Number of pages10
    JournalRSC Advances
    Volume3
    Issue number48
    DOIs
    StatePublished - Dec 28 2013

    Profile

    Ammonia
    Fibers
    Abnormal Erythrocytes
    Saimiriine herpesvirus 2
    Fermentation
    Enzymes
    Enzymatic hydrolysis
    Biofuels
    Sugars
    Seed
    Biomass
    Costs
    Panthera
    Proteomics

    ASJC Scopus subject areas

    • Chemical Engineering(all)
    • Chemistry(all)

    Cite this

    In-house cellulase production from AFEX™ pretreated corn stover using Trichoderma reesei RUT C-30. / Culbertson, Alan; Jin, Mingjie; Da Costa Sousa, Leonardo; Dale, Bruce E.; Balan, Venkatesh.

    In: RSC Advances, Vol. 3, No. 48, 28.12.2013, p. 25960-25969.

    Research output: Contribution to journalArticle

    Culbertson, Alan; Jin, Mingjie; Da Costa Sousa, Leonardo; Dale, Bruce E.; Balan, Venkatesh / In-house cellulase production from AFEX™ pretreated corn stover using Trichoderma reesei RUT C-30.

    In: RSC Advances, Vol. 3, No. 48, 28.12.2013, p. 25960-25969.

    Research output: Contribution to journalArticle

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    abstract = "Cellulase represents a major cost for biochemical conversion of lignocellulosic biomass to biofuels. In the present study, in-house production of cellulases from Ammonia Fiber Expansion pretreated corn stover (AFEX-CS) using Trichoderma reesei Rut C-30 was investigated. Seed culture was conducted in a simple medium containing corn steep liquor (CSL) and AFEX-CS hydrolysate. The effects of fermentation medium components (CSL, AFEX-CS, Tween 80 and CaCO3) on cellulase production were investigated and a simple fermentation medium was developed containing 20 g L-1 AFEX-CS and 4.0 g L-1 CaCO3. A cellulase activity of 1.5 FPU per mL was produced in this medium. Addition of 0.5% (v/v) CSL in the fermentation medium further improved cellulase activity (1.9 FPU per mL). Enzymatic hydrolysis of AFEX-CS using in-house produced enzymes generated high sugar yields with high enzyme loading. Proteomics analyses indicated that EGI and CBHII were deficient in the in-house produced enzyme cocktail when compared to the optimal cocktail for hydrolyzing AFEX-CS.",
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