In vitro binding of single-stranded RNA by human Dicer

Hemant K. Kini, S. Patrick Walton

Research output: Contribution to journalArticle

  • 15 Citations

Abstract

While Dicer alone has been shown to form stable complexes with double-stranded RNAs and short interfering RNAs, its interactions with single-stranded RNAs (ssRNAs) have not been characterized. Here, we show that recombinant human Dicer alone can bind 21-nt ssRNAs in vitro, independent of their sequence and structure. We also demonstrate that Dicer binds ssRNAs having a 5′-phosphate with greater affinity versus those with a 5′-hydroxyl. In addition, 3′-biotinylated ssRNAs are bound by Dicer with lower affinity than 3′-hydroxyl ssRNAs. The stability of ssRNA-Dicer complexes was found to depend on divalent cations. Together, our results suggest a role for the PAZ domain of Dicer in binding ssRNAs and may indicate roles for Dicer in cellular function beyond those currently known.

LanguageEnglish (US)
Pages5611-5616
Number of pages6
JournalFEBS Letters
Volume581
Issue number29
DOIs
StatePublished - Dec 11 2007

Profile

RNA
Hydroxyl Radical
Double-Stranded RNA
Divalent Cations
Small Interfering RNA
In Vitro Techniques
Phosphates

Keywords

  • Dicer
  • Divalent cation
  • PAZ domain
  • Single-stranded RNA
  • siRNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

In vitro binding of single-stranded RNA by human Dicer. / Kini, Hemant K.; Walton, S. Patrick.

In: FEBS Letters, Vol. 581, No. 29, 11.12.2007, p. 5611-5616.

Research output: Contribution to journalArticle

Kini, Hemant K. ; Walton, S. Patrick. / In vitro binding of single-stranded RNA by human Dicer. In: FEBS Letters. 2007 ; Vol. 581, No. 29. pp. 5611-5616
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