Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissue-engineered vascular grafts in vivo

G. N. Nelson, J. D. Roh, T. L. Mirensky, Y. Wang, T. Yi, G. Tellides, J. S. Pober, P. Shkarin, E. M. Shapiro, W. M. Saltzman, X. Papademetris, T. M. Fahmy, C. K. Breuer

Research output: Contribution to journalArticle

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Abstract

This pilot study examines noninvasive MR monitoring of tissue-engineered vascular grafts (TEVGs) in vivo using cells labeled with iron oxide nanoparticles. Human aortic smooth muscle cells (hASMCs) were labeled with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles. The labeled hASMCs, along with human aortic endothelial cells, were incorporated into eight TEVGs and were then surgically implanted as aortic interposition grafts in a C.B-17 SCID/bg mouse host. USPIO-labeled hASMCs persisted in the grafts throughout a 3 wk observation period and allowed noninvasive MR imaging of the human TEVGs for real-time, serial monitoring of hASMC retention. This study demonstrates the feasibility of applying noninvasive imaging techniques for evaluation of in vivo TEVG performance.

LanguageEnglish (US)
Pages3888-3895
Number of pages8
JournalFASEB Journal
Volume22
Issue number11
DOIs
StatePublished - Nov 2008
Externally publishedYes

Profile

Blood Vessel Prosthesis
Grafts
Labeling
Tissue
Smooth Muscle Myocytes
Muscle
Monitoring
Nanoparticles
Transplants
Imaging techniques
SCID Mice
Endothelial cells
Feasibility Studies
ferumoxtran-10
Endothelial Cells
Cells
Observation

Keywords

  • Animal model
  • Arterial grafts
  • MRI
  • SCID/bg

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology

Cite this

Nelson, G. N., Roh, J. D., Mirensky, T. L., Wang, Y., Yi, T., Tellides, G., ... Breuer, C. K. (2008). Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissue-engineered vascular grafts in vivo. FASEB Journal, 22(11), 3888-3895. DOI: 10.1096/fj.08-107367

Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissue-engineered vascular grafts in vivo. / Nelson, G. N.; Roh, J. D.; Mirensky, T. L.; Wang, Y.; Yi, T.; Tellides, G.; Pober, J. S.; Shkarin, P.; Shapiro, E. M.; Saltzman, W. M.; Papademetris, X.; Fahmy, T. M.; Breuer, C. K.

In: FASEB Journal, Vol. 22, No. 11, 11.2008, p. 3888-3895.

Research output: Contribution to journalArticle

Nelson, GN, Roh, JD, Mirensky, TL, Wang, Y, Yi, T, Tellides, G, Pober, JS, Shkarin, P, Shapiro, EM, Saltzman, WM, Papademetris, X, Fahmy, TM & Breuer, CK 2008, 'Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissue-engineered vascular grafts in vivo' FASEB Journal, vol. 22, no. 11, pp. 3888-3895. DOI: 10.1096/fj.08-107367
Nelson GN, Roh JD, Mirensky TL, Wang Y, Yi T, Tellides G et al. Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissue-engineered vascular grafts in vivo. FASEB Journal. 2008 Nov;22(11):3888-3895. Available from, DOI: 10.1096/fj.08-107367
Nelson, G. N. ; Roh, J. D. ; Mirensky, T. L. ; Wang, Y. ; Yi, T. ; Tellides, G. ; Pober, J. S. ; Shkarin, P. ; Shapiro, E. M. ; Saltzman, W. M. ; Papademetris, X. ; Fahmy, T. M. ; Breuer, C. K./ Initial evaluation of the use of USPIO cell labeling and noninvasive MR monitoring of human tissue-engineered vascular grafts in vivo. In: FASEB Journal. 2008 ; Vol. 22, No. 11. pp. 3888-3895
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