Insulin concentration during preconditioning mediates the regulation of urea synthesis during exposure to amino acid-supplemented plasma

Zheng Li, Martin L. Yarmush, Christina Chan

Research output: Contribution to journalArticle

  • 7 Citations

Abstract

Understanding the effects of preconditioning on cellular metabolism is important in providing insight into how cells and tissues may behave when confronted with alterations in their environment. Previously, elevated accumulation of lipids and a correspondingly reduced rate of urea synthesis were found in primary heptocytes preconditioned in culture medium containing high levels of insulin and then exposed to plasma. Subsequent studies found that preconditioning primary hepatocytes in medium containing low levels of insulin before exposure to plasma supplemented with amino acids was able to confer resistance to the lipid-accumulating effect of plasma exposure and restored urea synthesis. In the current study, we investigated the effects of insulin preconditioning and amino acid supplementation on the gene expression profile of the urea cycle and fatty acid metabolism enzymes. We found that insulin preconditioning mediates the effects of amino acids in the plasma exposure period on urea synthesis. Urea synthesis is regulated by insulin and amino acids through both short-term and long-term control. Possible mechanisms of long-term control through transcriptional regulation of urea synthesis by insulin, amino acids, and enzymes and transcription factors associated with lipid metabolism are discussed.

LanguageEnglish (US)
Pages1737-1746
Number of pages10
JournalTissue Engineering
Volume10
Issue number11-12
StatePublished - Nov 2004

Profile

Insulin
Urea
Amino acids
Plasmas
Amino Acids
Metabolism
Lipids
Enzymes
Transcription factors
Transcriptome
Lipid Metabolism
Fatty acids
Gene expression
Culture Media
Hepatocytes
Transcription Factors
Fatty Acids
Tissue

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Biotechnology

Cite this

Insulin concentration during preconditioning mediates the regulation of urea synthesis during exposure to amino acid-supplemented plasma. / Li, Zheng; Yarmush, Martin L.; Chan, Christina.

In: Tissue Engineering, Vol. 10, No. 11-12, 11.2004, p. 1737-1746.

Research output: Contribution to journalArticle

@article{31e47258c8bf4394b89feaab728e829c,
title = "Insulin concentration during preconditioning mediates the regulation of urea synthesis during exposure to amino acid-supplemented plasma",
abstract = "Understanding the effects of preconditioning on cellular metabolism is important in providing insight into how cells and tissues may behave when confronted with alterations in their environment. Previously, elevated accumulation of lipids and a correspondingly reduced rate of urea synthesis were found in primary heptocytes preconditioned in culture medium containing high levels of insulin and then exposed to plasma. Subsequent studies found that preconditioning primary hepatocytes in medium containing low levels of insulin before exposure to plasma supplemented with amino acids was able to confer resistance to the lipid-accumulating effect of plasma exposure and restored urea synthesis. In the current study, we investigated the effects of insulin preconditioning and amino acid supplementation on the gene expression profile of the urea cycle and fatty acid metabolism enzymes. We found that insulin preconditioning mediates the effects of amino acids in the plasma exposure period on urea synthesis. Urea synthesis is regulated by insulin and amino acids through both short-term and long-term control. Possible mechanisms of long-term control through transcriptional regulation of urea synthesis by insulin, amino acids, and enzymes and transcription factors associated with lipid metabolism are discussed.",
author = "Zheng Li and Yarmush, {Martin L.} and Christina Chan",
year = "2004",
month = "11",
language = "English (US)",
volume = "10",
pages = "1737--1746",
journal = "Tissue Engineering",
issn = "1076-3279",
publisher = "Mary Ann Liebert Inc.",
number = "11-12",

}

TY - JOUR

T1 - Insulin concentration during preconditioning mediates the regulation of urea synthesis during exposure to amino acid-supplemented plasma

AU - Li,Zheng

AU - Yarmush,Martin L.

AU - Chan,Christina

PY - 2004/11

Y1 - 2004/11

N2 - Understanding the effects of preconditioning on cellular metabolism is important in providing insight into how cells and tissues may behave when confronted with alterations in their environment. Previously, elevated accumulation of lipids and a correspondingly reduced rate of urea synthesis were found in primary heptocytes preconditioned in culture medium containing high levels of insulin and then exposed to plasma. Subsequent studies found that preconditioning primary hepatocytes in medium containing low levels of insulin before exposure to plasma supplemented with amino acids was able to confer resistance to the lipid-accumulating effect of plasma exposure and restored urea synthesis. In the current study, we investigated the effects of insulin preconditioning and amino acid supplementation on the gene expression profile of the urea cycle and fatty acid metabolism enzymes. We found that insulin preconditioning mediates the effects of amino acids in the plasma exposure period on urea synthesis. Urea synthesis is regulated by insulin and amino acids through both short-term and long-term control. Possible mechanisms of long-term control through transcriptional regulation of urea synthesis by insulin, amino acids, and enzymes and transcription factors associated with lipid metabolism are discussed.

AB - Understanding the effects of preconditioning on cellular metabolism is important in providing insight into how cells and tissues may behave when confronted with alterations in their environment. Previously, elevated accumulation of lipids and a correspondingly reduced rate of urea synthesis were found in primary heptocytes preconditioned in culture medium containing high levels of insulin and then exposed to plasma. Subsequent studies found that preconditioning primary hepatocytes in medium containing low levels of insulin before exposure to plasma supplemented with amino acids was able to confer resistance to the lipid-accumulating effect of plasma exposure and restored urea synthesis. In the current study, we investigated the effects of insulin preconditioning and amino acid supplementation on the gene expression profile of the urea cycle and fatty acid metabolism enzymes. We found that insulin preconditioning mediates the effects of amino acids in the plasma exposure period on urea synthesis. Urea synthesis is regulated by insulin and amino acids through both short-term and long-term control. Possible mechanisms of long-term control through transcriptional regulation of urea synthesis by insulin, amino acids, and enzymes and transcription factors associated with lipid metabolism are discussed.

UR - http://www.scopus.com/inward/record.url?scp=11144242555&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11144242555&partnerID=8YFLogxK

M3 - Article

VL - 10

SP - 1737

EP - 1746

JO - Tissue Engineering

T2 - Tissue Engineering

JF - Tissue Engineering

SN - 1076-3279

IS - 11-12

ER -