Insulin concentration during preconditioning mediates the regulation of urea synthesis during exposure to amino acid-supplemented plasma

Zheng Li, Martin L. Yarmush, Christina Chan

    Research output: Contribution to journalArticle

    • 7 Citations

    Abstract

    Understanding the effects of preconditioning on cellular metabolism is important in providing insight into how cells and tissues may behave when confronted with alterations in their environment. Previously, elevated accumulation of lipids and a correspondingly reduced rate of urea synthesis were found in primary heptocytes preconditioned in culture medium containing high levels of insulin and then exposed to plasma. Subsequent studies found that preconditioning primary hepatocytes in medium containing low levels of insulin before exposure to plasma supplemented with amino acids was able to confer resistance to the lipid-accumulating effect of plasma exposure and restored urea synthesis. In the current study, we investigated the effects of insulin preconditioning and amino acid supplementation on the gene expression profile of the urea cycle and fatty acid metabolism enzymes. We found that insulin preconditioning mediates the effects of amino acids in the plasma exposure period on urea synthesis. Urea synthesis is regulated by insulin and amino acids through both short-term and long-term control. Possible mechanisms of long-term control through transcriptional regulation of urea synthesis by insulin, amino acids, and enzymes and transcription factors associated with lipid metabolism are discussed.

    Original languageEnglish (US)
    Pages (from-to)1737-1746
    Number of pages10
    JournalTissue Engineering
    Volume10
    Issue number11-12
    StatePublished - Nov 2004

    Profile

    Insulin
    Urea
    Amino Acids
    Amino acids
    Plasmas
    Lipids
    Enzymes
    Metabolism
    Lipid Metabolism
    Transcriptome
    Culture Media
    Hepatocytes
    Transcription Factors
    Fatty Acids
    Transcription factors
    Fatty acids
    Gene expression
    Tissue

    ASJC Scopus subject areas

    • Biophysics
    • Cell Biology
    • Biotechnology

    Cite this

    Insulin concentration during preconditioning mediates the regulation of urea synthesis during exposure to amino acid-supplemented plasma. / Li, Zheng; Yarmush, Martin L.; Chan, Christina.

    In: Tissue Engineering, Vol. 10, No. 11-12, 11.2004, p. 1737-1746.

    Research output: Contribution to journalArticle

    Li, Zheng; Yarmush, Martin L.; Chan, Christina / Insulin concentration during preconditioning mediates the regulation of urea synthesis during exposure to amino acid-supplemented plasma.

    In: Tissue Engineering, Vol. 10, No. 11-12, 11.2004, p. 1737-1746.

    Research output: Contribution to journalArticle

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