IPAF inflammasome is involved in interleukin-1β production from astrocytes, induced by palmitate; implications for Alzheimer's Disease

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    Abstract

    Inflammatory response has been strongly implicated in the pathogenesis of numerous diseases, including Alzheimer's disease (AD). However, little is known about the molecular mechanisms initiating the generation of inflammatory molecules in the central nervous system, such as interleukin-1β (IL-1β). Previously we identified that palmitate can induce primary astrocytes to produce cytokines, causing AD-like changes in primary neurons. Here we investigated and identified that palmitate induced the activation of ice protease-activating factor (IPAF)-apoptosis-associated speck-like protein containing a caspase activation and recruitment domains (CARD) (ASC) inflammasome in astrocytes leading to the maturation of IL-1β, thereby implicating that not only pathogen-related factors can activate the IPAF-ASC inflammasome. Moreover, downregulating IPAF (which was found to be regulated by cAMP response element-binding protein) in astrocytes through silencing to decrease IL-1β secretion from the astrocytes reduced the generation of amyloid-β42 by primary neurons. Furthermore, the expression levels of IPAF and ASC were found significantly elevated in a subgroup of sporadic AD patients, suggesting an involvement of the IPAF-ASC inflammasome in the inflammatory response associated with AD, and thus could be a potential therapeutic target for AD.

    Original languageEnglish (US)
    Pages (from-to)309-321
    Number of pages13
    JournalNeurobiology of Aging
    Volume35
    Issue number2
    DOIs
    StatePublished - Feb 2014

    Profile

    Inflammasomes
    Palmitates
    Ice
    Interleukin-1beta
    Astrocytes
    Alzheimer Disease
    Peptide Hydrolases
    Neurons
    Cyclic AMP Response Element-Binding Protein
    Caspases
    Amyloid
    Down-Regulation
    Central Nervous System
    Apoptosis
    Cytokines
    Proteins

    Keywords

    • Alzheimer's disease
    • Fatty acid
    • IL-1β
    • Inflammasome
    • IPAF

    ASJC Scopus subject areas

    • Clinical Neurology
    • Neuroscience(all)
    • Aging
    • Developmental Biology
    • Geriatrics and Gerontology

    Cite this

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    title = "IPAF inflammasome is involved in interleukin-1β production from astrocytes, induced by palmitate; implications for Alzheimer's Disease",
    abstract = "Inflammatory response has been strongly implicated in the pathogenesis of numerous diseases, including Alzheimer's disease (AD). However, little is known about the molecular mechanisms initiating the generation of inflammatory molecules in the central nervous system, such as interleukin-1β (IL-1β). Previously we identified that palmitate can induce primary astrocytes to produce cytokines, causing AD-like changes in primary neurons. Here we investigated and identified that palmitate induced the activation of ice protease-activating factor (IPAF)-apoptosis-associated speck-like protein containing a caspase activation and recruitment domains (CARD) (ASC) inflammasome in astrocytes leading to the maturation of IL-1β, thereby implicating that not only pathogen-related factors can activate the IPAF-ASC inflammasome. Moreover, downregulating IPAF (which was found to be regulated by cAMP response element-binding protein) in astrocytes through silencing to decrease IL-1β secretion from the astrocytes reduced the generation of amyloid-β42 by primary neurons. Furthermore, the expression levels of IPAF and ASC were found significantly elevated in a subgroup of sporadic AD patients, suggesting an involvement of the IPAF-ASC inflammasome in the inflammatory response associated with AD, and thus could be a potential therapeutic target for AD.",
    keywords = "Alzheimer's disease, Fatty acid, IL-1β, Inflammasome, IPAF",
    author = "Li Liu and Christina Chan",
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    KW - IPAF

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