Magnetic cell labeling of primary and stem cell-derived pig hepatocytes for MRI-based cell tracking of hepatocyte transplantation

Dwayne R. Roach, Wesley M. Garrett, Glenn Welch, Thomas J. Caperna, Neil C. Talbot, Erik M. Shapiro

Research output: Contribution to journalArticle

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Abstract

Pig hepatocytes are an important investigational tool for optimizing hepatocyte transplantation schemes in both allogeneic and xenogeneic transplant scenarios. MRI can be used to serially monitor the transplanted cells, but only if the hepatocytes can be labeled with a magnetic particle. In this work, we describe culture conditions for magnetic cell labeling of cells from two different pig hepatocyte cell sources; primary pig hepatocytes (ppHEP) and stem cell-derived hepatocytes (PICM-19FF). The magnetic particle is a micron-sized iron oxide particle (MPIO) that has been extensively studied for magnetic cell labeling for MRI-based cell tracking. ppHEP could endocytose MPIO with labeling percentages as high as 70%, achieving iron content as high as ∼55 pg/cell, with > 75% viability. PICM-19FF had labeling >97%, achieving iron content ∼38 pg/cell, with viability >99%. Extensive morphological and functional assays indicated that magnetic cell labeling was benign to the cells. The results encourage the use of MRI-based cell tracking for the development and clinical use of hepatocyte transplantation methodologies. Further, these results generally highlight the importance of functional cell assays in the evaluation of contrast agent biocompatibility.

LanguageEnglish (US)
Article numbere0123282
JournalPLoS One
Volume10
Issue number4
DOIs
StatePublished - Apr 9 2015

Profile

Cell Tracking
Stem cells
Magnetic resonance imaging
Labeling
hepatocytes
stem cells
Hepatocytes
Swine
Stem Cells
Transplantation
swine
cells
Assays
Iron
Transplantation (surgical)
Transplants
Biocompatibility
viability
iron
Contrast Media

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Magnetic cell labeling of primary and stem cell-derived pig hepatocytes for MRI-based cell tracking of hepatocyte transplantation. / Roach, Dwayne R.; Garrett, Wesley M.; Welch, Glenn; Caperna, Thomas J.; Talbot, Neil C.; Shapiro, Erik M.

In: PLoS One, Vol. 10, No. 4, e0123282, 09.04.2015.

Research output: Contribution to journalArticle

Roach, Dwayne R. ; Garrett, Wesley M. ; Welch, Glenn ; Caperna, Thomas J. ; Talbot, Neil C. ; Shapiro, Erik M./ Magnetic cell labeling of primary and stem cell-derived pig hepatocytes for MRI-based cell tracking of hepatocyte transplantation. In: PLoS One. 2015 ; Vol. 10, No. 4.
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