Magnetic cell labeling of primary and stem cell-derived pig hepatocytes for MRI-based cell tracking of hepatocyte transplantation

Dwayne R. Roach, Wesley M. Garrett, Glenn Welch, Thomas J. Caperna, Neil C. Talbot, Erik M. Shapiro

    Research output: Contribution to journalArticle

    • 4 Citations

    Abstract

    Pig hepatocytes are an important investigational tool for optimizing hepatocyte transplantation schemes in both allogeneic and xenogeneic transplant scenarios. MRI can be used to serially monitor the transplanted cells, but only if the hepatocytes can be labeled with a magnetic particle. In this work, we describe culture conditions for magnetic cell labeling of cells from two different pig hepatocyte cell sources; primary pig hepatocytes (ppHEP) and stem cell-derived hepatocytes (PICM-19FF). The magnetic particle is a micron-sized iron oxide particle (MPIO) that has been extensively studied for magnetic cell labeling for MRI-based cell tracking. ppHEP could endocytose MPIO with labeling percentages as high as 70%, achieving iron content as high as ∼55 pg/cell, with > 75% viability. PICM-19FF had labeling >97%, achieving iron content ∼38 pg/cell, with viability >99%. Extensive morphological and functional assays indicated that magnetic cell labeling was benign to the cells. The results encourage the use of MRI-based cell tracking for the development and clinical use of hepatocyte transplantation methodologies. Further, these results generally highlight the importance of functional cell assays in the evaluation of contrast agent biocompatibility.

    Original languageEnglish (US)
    Article numbere0123282
    JournalPLoS One
    Volume10
    Issue number4
    DOIs
    StatePublished - Apr 9 2015

    Profile

    Cell Tracking
    Hepatocytes
    Swine
    Stem Cells
    Transplantation
    cells
    hepatocytes
    Mastication
    Magnetic resonance imaging
    swine
    Iron
    Beryllium
    Status Epilepticus
    stem cells
    viability
    iron
    assays
    Endocytosis
    Oxides
    Contrast Media

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Magnetic cell labeling of primary and stem cell-derived pig hepatocytes for MRI-based cell tracking of hepatocyte transplantation. / Roach, Dwayne R.; Garrett, Wesley M.; Welch, Glenn; Caperna, Thomas J.; Talbot, Neil C.; Shapiro, Erik M.

    In: PLoS One, Vol. 10, No. 4, e0123282, 09.04.2015.

    Research output: Contribution to journalArticle

    Roach, Dwayne R.; Garrett, Wesley M.; Welch, Glenn; Caperna, Thomas J.; Talbot, Neil C.; Shapiro, Erik M. / Magnetic cell labeling of primary and stem cell-derived pig hepatocytes for MRI-based cell tracking of hepatocyte transplantation.

    In: PLoS One, Vol. 10, No. 4, e0123282, 09.04.2015.

    Research output: Contribution to journalArticle

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