Magnetic poly(lactide-co-glycolide) and cellulose particles for MRI-based cell tracking

Michael K. Nkansah, Durga Thakral, Erik M. Shapiro

Research output: Contribution to journalArticle

  • 47 Citations

Abstract

Biodegradable, superparamagnetic microparticles and nanoparticles of poly(lactide-co-glycolide) (PLGA) and cellulose were designed, fabricated, and characterized for magnetic cell labeling. Monodisperse nanocrystals of magnetite were incorporated into microparticles and nanoparticles of PLGA and cellulose with high efficiency using an oil-in-water single emulsion technique. Superparamagnetic cores had high magnetization (72.1 emu/g). The resulting polymeric particles had smooth surface morphology and high magnetite content (43.3 wt % for PLGA and 69.6 wt % for cellulose). While PLGA and cellulose nanoparticles displayed highest r*2 values per millimole of iron (399 sec-1 mM-1 for cellulose and 505 sec -1 mM-1 for PLGA), micron-sized PLGA particles had a much higher r*2 per particle than either. After incubation for a month in citrate buffer (pH 5.5), magnetic PLGA particles lost close to 50% of their initial r*2 molar relaxivity, while magnetic cellulose particles remained intact, preserving over 85% of their initial r*2 molar relaxivity. Lastly, mesenchymal stem cells and human breast adenocarcinoma cells were magnetically labeled using these particles with no detectable cytotoxicity. These particles are ideally suited for noninvasive cell tracking in vivo via MRI and due to their vastly different degradation properties, offer unique potential for dedicated use for either short (PLGA-based particles) or long-term (cellulose-based particles) experiments.

Original languageEnglish (US)
Pages (from-to)1776-1785
Number of pages10
JournalMagnetic Resonance in Medicine
Volume65
Issue number6
DOIs
StatePublished - Jun 2011
Externally publishedYes

Profile

Cell Tracking
Polyglactin 910
Cellulose
Nanoparticles
Ferrosoferric Oxide
Dromaiidae
Emulsions
Mesenchymal Stromal Cells
Citric Acid
Buffers
Oils
Adenocarcinoma
Breast
Iron
Water

Keywords

  • cell tracking
  • iron oxide
  • MPIO
  • MRI
  • SPIO

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Magnetic poly(lactide-co-glycolide) and cellulose particles for MRI-based cell tracking. / Nkansah, Michael K.; Thakral, Durga; Shapiro, Erik M.

In: Magnetic Resonance in Medicine, Vol. 65, No. 6, 06.2011, p. 1776-1785.

Research output: Contribution to journalArticle

Nkansah, Michael K.; Thakral, Durga; Shapiro, Erik M. / Magnetic poly(lactide-co-glycolide) and cellulose particles for MRI-based cell tracking.

In: Magnetic Resonance in Medicine, Vol. 65, No. 6, 06.2011, p. 1776-1785.

Research output: Contribution to journalArticle

@article{d18105d5a3be458bb83a0c4e02308e44,
title = "Magnetic poly(lactide-co-glycolide) and cellulose particles for MRI-based cell tracking",
abstract = "Biodegradable, superparamagnetic microparticles and nanoparticles of poly(lactide-co-glycolide) (PLGA) and cellulose were designed, fabricated, and characterized for magnetic cell labeling. Monodisperse nanocrystals of magnetite were incorporated into microparticles and nanoparticles of PLGA and cellulose with high efficiency using an oil-in-water single emulsion technique. Superparamagnetic cores had high magnetization (72.1 emu/g). The resulting polymeric particles had smooth surface morphology and high magnetite content (43.3 wt % for PLGA and 69.6 wt % for cellulose). While PLGA and cellulose nanoparticles displayed highest r*2 values per millimole of iron (399 sec-1 mM-1 for cellulose and 505 sec -1 mM-1 for PLGA), micron-sized PLGA particles had a much higher r*2 per particle than either. After incubation for a month in citrate buffer (pH 5.5), magnetic PLGA particles lost close to 50% of their initial r*2 molar relaxivity, while magnetic cellulose particles remained intact, preserving over 85% of their initial r*2 molar relaxivity. Lastly, mesenchymal stem cells and human breast adenocarcinoma cells were magnetically labeled using these particles with no detectable cytotoxicity. These particles are ideally suited for noninvasive cell tracking in vivo via MRI and due to their vastly different degradation properties, offer unique potential for dedicated use for either short (PLGA-based particles) or long-term (cellulose-based particles) experiments.",
keywords = "cell tracking, iron oxide, MPIO, MRI, SPIO",
author = "Nkansah, {Michael K.} and Durga Thakral and Shapiro, {Erik M.}",
year = "2011",
month = "6",
doi = "10.1002/mrm.22765",
volume = "65",
pages = "1776--1785",
journal = "Magnetic Resonance in Medicine",
issn = "0740-3194",
publisher = "John Wiley and Sons Inc.",
number = "6",

}

TY - JOUR

T1 - Magnetic poly(lactide-co-glycolide) and cellulose particles for MRI-based cell tracking

AU - Nkansah,Michael K.

AU - Thakral,Durga

AU - Shapiro,Erik M.

PY - 2011/6

Y1 - 2011/6

N2 - Biodegradable, superparamagnetic microparticles and nanoparticles of poly(lactide-co-glycolide) (PLGA) and cellulose were designed, fabricated, and characterized for magnetic cell labeling. Monodisperse nanocrystals of magnetite were incorporated into microparticles and nanoparticles of PLGA and cellulose with high efficiency using an oil-in-water single emulsion technique. Superparamagnetic cores had high magnetization (72.1 emu/g). The resulting polymeric particles had smooth surface morphology and high magnetite content (43.3 wt % for PLGA and 69.6 wt % for cellulose). While PLGA and cellulose nanoparticles displayed highest r*2 values per millimole of iron (399 sec-1 mM-1 for cellulose and 505 sec -1 mM-1 for PLGA), micron-sized PLGA particles had a much higher r*2 per particle than either. After incubation for a month in citrate buffer (pH 5.5), magnetic PLGA particles lost close to 50% of their initial r*2 molar relaxivity, while magnetic cellulose particles remained intact, preserving over 85% of their initial r*2 molar relaxivity. Lastly, mesenchymal stem cells and human breast adenocarcinoma cells were magnetically labeled using these particles with no detectable cytotoxicity. These particles are ideally suited for noninvasive cell tracking in vivo via MRI and due to their vastly different degradation properties, offer unique potential for dedicated use for either short (PLGA-based particles) or long-term (cellulose-based particles) experiments.

AB - Biodegradable, superparamagnetic microparticles and nanoparticles of poly(lactide-co-glycolide) (PLGA) and cellulose were designed, fabricated, and characterized for magnetic cell labeling. Monodisperse nanocrystals of magnetite were incorporated into microparticles and nanoparticles of PLGA and cellulose with high efficiency using an oil-in-water single emulsion technique. Superparamagnetic cores had high magnetization (72.1 emu/g). The resulting polymeric particles had smooth surface morphology and high magnetite content (43.3 wt % for PLGA and 69.6 wt % for cellulose). While PLGA and cellulose nanoparticles displayed highest r*2 values per millimole of iron (399 sec-1 mM-1 for cellulose and 505 sec -1 mM-1 for PLGA), micron-sized PLGA particles had a much higher r*2 per particle than either. After incubation for a month in citrate buffer (pH 5.5), magnetic PLGA particles lost close to 50% of their initial r*2 molar relaxivity, while magnetic cellulose particles remained intact, preserving over 85% of their initial r*2 molar relaxivity. Lastly, mesenchymal stem cells and human breast adenocarcinoma cells were magnetically labeled using these particles with no detectable cytotoxicity. These particles are ideally suited for noninvasive cell tracking in vivo via MRI and due to their vastly different degradation properties, offer unique potential for dedicated use for either short (PLGA-based particles) or long-term (cellulose-based particles) experiments.

KW - cell tracking

KW - iron oxide

KW - MPIO

KW - MRI

KW - SPIO

UR - http://www.scopus.com/inward/record.url?scp=79958257811&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79958257811&partnerID=8YFLogxK

U2 - 10.1002/mrm.22765

DO - 10.1002/mrm.22765

M3 - Article

VL - 65

SP - 1776

EP - 1785

JO - Magnetic Resonance in Medicine

T2 - Magnetic Resonance in Medicine

JF - Magnetic Resonance in Medicine

SN - 0740-3194

IS - 6

ER -