Magnetic resonance imaging of the migration of neuronal precursors generated in the adult rodent brain

Erik M. Shapiro, Oscar Gonzalez-Perez, Jose Manuel García-Verdugo, Arturo Alvarez-Buylla, Alan P. Koretsky

Research output: Contribution to journalArticle

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Abstract

Neural progenitor cells (NPCs) reside within the subventricular zone (SVZ) in rodents. These NPCs give rise to neural precursors in adults that migrate to the olfactory bulb (OB) along a well-defined pathway, the rostral migratory stream (RMS). Here we demonstrate that these NPCs can be labeled, in vivo, in adult rats with fluorescent, micron-sized iron oxide particles (MPIOs), and that magnetic resonance imaging (MRI) can detect migrating neural precursors carrying MPIOs along the RMS to the OB. Immunohistochemistry and electron microscopy indicated that particles were inside GFAP+ neural progenitor cells in the SVZ, migrating PSA-NCAM+ and Doublecortin+ neural precursors within the RMS and OB, and Neu-N+ mature neurons in the OB. This work demonstrates that in vivo cell labeling of progenitor cells for MRI is possible and enables the serial, non-invasive visualization of endogenous progenitor/precursor cell migration.

Original languageEnglish (US)
Pages (from-to)1150-1157
Number of pages8
JournalNeuroImage
Volume32
Issue number3
DOIs
StatePublished - Sep 2006
Externally publishedYes

Profile

Rodentia
Stem Cells
Magnetic Resonance Imaging
Brain
Olfactory Bulb
Lateral Ventricles
Neural Cell Adhesion Molecules
Oxides
Cell Movement
Electron Microscopy
Iron
Immunohistochemistry
Neurons

Keywords

  • Contrast agents
  • Iron oxide
  • MRI
  • Neurons
  • Stem cells

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Neurology

Cite this

Shapiro, E. M., Gonzalez-Perez, O., Manuel García-Verdugo, J., Alvarez-Buylla, A., & Koretsky, A. P. (2006). Magnetic resonance imaging of the migration of neuronal precursors generated in the adult rodent brain. NeuroImage, 32(3), 1150-1157. DOI: 10.1016/j.neuroimage.2006.04.219

Magnetic resonance imaging of the migration of neuronal precursors generated in the adult rodent brain. / Shapiro, Erik M.; Gonzalez-Perez, Oscar; Manuel García-Verdugo, Jose; Alvarez-Buylla, Arturo; Koretsky, Alan P.

In: NeuroImage, Vol. 32, No. 3, 09.2006, p. 1150-1157.

Research output: Contribution to journalArticle

Shapiro, EM, Gonzalez-Perez, O, Manuel García-Verdugo, J, Alvarez-Buylla, A & Koretsky, AP 2006, 'Magnetic resonance imaging of the migration of neuronal precursors generated in the adult rodent brain' NeuroImage, vol 32, no. 3, pp. 1150-1157. DOI: 10.1016/j.neuroimage.2006.04.219
Shapiro EM, Gonzalez-Perez O, Manuel García-Verdugo J, Alvarez-Buylla A, Koretsky AP. Magnetic resonance imaging of the migration of neuronal precursors generated in the adult rodent brain. NeuroImage. 2006 Sep;32(3):1150-1157. Available from, DOI: 10.1016/j.neuroimage.2006.04.219

Shapiro, Erik M.; Gonzalez-Perez, Oscar; Manuel García-Verdugo, Jose; Alvarez-Buylla, Arturo; Koretsky, Alan P. / Magnetic resonance imaging of the migration of neuronal precursors generated in the adult rodent brain.

In: NeuroImage, Vol. 32, No. 3, 09.2006, p. 1150-1157.

Research output: Contribution to journalArticle

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