Maintaining high anaerobic succinic acid productivity by product removal

Christian Andersson, Ekaterina Petrova, Kris Berglund, Ulrika Rova

Research output: Contribution to journalArticle

  • 14 Citations

Abstract

During dual-phase fermentations using Escherichia coli engineered for succinic acid production, the productivity and viable cell concentration decrease as the concentration of succinic acid increases. The effects of succinic acid on the fermentation kinetics, yield, and cell viability were investigated by resuspending cells in fresh media after selected fermentation times. The cellular succinic acid productivity could be restored, but cell viability continuously decreased throughout the fermentations by up to 80% and subsequently the volumetric productivity was reduced. Omitting complex nutrients in the resuspension media had no significant effect on cellular succinate productivity and yield, although the viable cell concentration and thus the volumetric productivity was reduced by approximately 20%. By resuspending the cells, the amount of succinate produced during a 100-h fermentation was increased by more than 60%. The results demonstrate that by product removal succinic acid productivity can be maintained at high levels for extended periods of time.

LanguageEnglish (US)
Pages711-718
Number of pages8
JournalBioprocess and Biosystems Engineering
Volume33
Issue number6
DOIs
StatePublished - Aug 2010

Profile

Succinic Acid
Byproducts
Fermentation
Productivity
Acids
Cells
Cell Survival
Enzyme kinetics
Escherichia coli
Nutrients
Food

Keywords

  • Cell resuspension
  • Cell viability
  • Fermentation
  • Productivity
  • Succinic acid

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Medicine(all)

Cite this

Maintaining high anaerobic succinic acid productivity by product removal. / Andersson, Christian; Petrova, Ekaterina; Berglund, Kris; Rova, Ulrika.

In: Bioprocess and Biosystems Engineering, Vol. 33, No. 6, 08.2010, p. 711-718.

Research output: Contribution to journalArticle

Andersson, Christian ; Petrova, Ekaterina ; Berglund, Kris ; Rova, Ulrika. / Maintaining high anaerobic succinic acid productivity by product removal. In: Bioprocess and Biosystems Engineering. 2010 ; Vol. 33, No. 6. pp. 711-718
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