Maintaining high anaerobic succinic acid productivity by product removal

Christian Andersson, Ekaterina Petrova, Kris Berglund, Ulrika Rova

    Research output: Research - peer-reviewArticle

    • 14 Citations

    Abstract

    During dual-phase fermentations using Escherichia coli engineered for succinic acid production, the productivity and viable cell concentration decrease as the concentration of succinic acid increases. The effects of succinic acid on the fermentation kinetics, yield, and cell viability were investigated by resuspending cells in fresh media after selected fermentation times. The cellular succinic acid productivity could be restored, but cell viability continuously decreased throughout the fermentations by up to 80% and subsequently the volumetric productivity was reduced. Omitting complex nutrients in the resuspension media had no significant effect on cellular succinate productivity and yield, although the viable cell concentration and thus the volumetric productivity was reduced by approximately 20%. By resuspending the cells, the amount of succinate produced during a 100-h fermentation was increased by more than 60%. The results demonstrate that by product removal succinic acid productivity can be maintained at high levels for extended periods of time.

    LanguageEnglish (US)
    Pages711-718
    Number of pages8
    JournalBioprocess and Biosystems Engineering
    Volume33
    Issue number6
    DOIs
    StatePublished - Aug 2010

    Profile

    Byproducts
    Productivity
    Acids
    Succinic Acid
    Fermentation
    Cells
    Cell Survival
    Enzyme kinetics
    Escherichia coli
    Nutrients
    Food

    Keywords

    • Cell resuspension
    • Cell viability
    • Fermentation
    • Productivity
    • Succinic acid

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Medicine(all)

    Cite this

    Maintaining high anaerobic succinic acid productivity by product removal. / Andersson, Christian; Petrova, Ekaterina; Berglund, Kris; Rova, Ulrika.

    In: Bioprocess and Biosystems Engineering, Vol. 33, No. 6, 08.2010, p. 711-718.

    Research output: Research - peer-reviewArticle

    Andersson, Christian ; Petrova, Ekaterina ; Berglund, Kris ; Rova, Ulrika. / Maintaining high anaerobic succinic acid productivity by product removal. In: Bioprocess and Biosystems Engineering. 2010 ; Vol. 33, No. 6. pp. 711-718
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