Mechanical nanosensor based on fret within a thermosome: Damage-reporting polymeric materials

Nico Bruns, Katarzyna Pustelny, Lisa M. Bergeron, Timothy A. Whitehead, Douglas S. Clark

Research output: Contribution to journalArticle

  • 52 Citations

Abstract

Under stress: Changes in stress of the polymer matrix in a protein-polymer hybrid material result in changes of conformation of the protein complex, thus resulting in a damage-reporting material (see picture). The reporter is an engineered chaperonin that covalently entraps a pair of fluorescent proteins. Deformation of the chaperonin leads to a change in fluorophore distance and a change in the fluorescene resonance energy transfer (FRET) signal.

Original languageEnglish (US)
Pages (from-to)5666-5669
Number of pages4
JournalAngewandte Chemie - International Edition
Volume48
Issue number31
DOIs
StatePublished - Jul 20 2009
Externally publishedYes

Profile

Thermosomes
Chaperonins
Polymers
Proteins
Addison Disease
Protein Conformation
Energy Transfer
Birth Injuries
Carbamyl Phosphate
Nanosensors
Diltiazem
Fluorophores
Traffic Accidents
Polymer matrix
Heme
Carotid Arteries
Hybrid materials
Energy transfer
Conformations

Keywords

  • Fret
  • Hybrid materials
  • Polymers
  • Proteins
  • Stress detection

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Medicine(all)

Cite this

Mechanical nanosensor based on fret within a thermosome : Damage-reporting polymeric materials. / Bruns, Nico; Pustelny, Katarzyna; Bergeron, Lisa M.; Whitehead, Timothy A.; Clark, Douglas S.

In: Angewandte Chemie - International Edition, Vol. 48, No. 31, 20.07.2009, p. 5666-5669.

Research output: Contribution to journalArticle

Bruns, Nico; Pustelny, Katarzyna; Bergeron, Lisa M.; Whitehead, Timothy A.; Clark, Douglas S. / Mechanical nanosensor based on fret within a thermosome : Damage-reporting polymeric materials.

In: Angewandte Chemie - International Edition, Vol. 48, No. 31, 20.07.2009, p. 5666-5669.

Research output: Contribution to journalArticle

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