Mechanical nanosensor based on fret within a thermosome: Damage-reporting polymeric materials

Nico Bruns, Katarzyna Pustelny, Lisa M. Bergeron, Timothy A. Whitehead, Douglas S. Clark

Research output: Research - peer-reviewArticle

  • 54 Citations

Abstract

Under stress: Changes in stress of the polymer matrix in a protein-polymer hybrid material result in changes of conformation of the protein complex, thus resulting in a damage-reporting material (see picture). The reporter is an engineered chaperonin that covalently entraps a pair of fluorescent proteins. Deformation of the chaperonin leads to a change in fluorophore distance and a change in the fluorescene resonance energy transfer (FRET) signal.

LanguageEnglish (US)
Pages5666-5669
Number of pages4
JournalAngewandte Chemie - International Edition
Volume48
Issue number31
DOIs
StatePublished - Jul 20 2009
Externally publishedYes

Profile

Thermosomes
Nanosensors
Polymers
Proteins
Chaperonins
Protein Conformation
Energy Transfer
Fluorophores
Hybrid materials
Polymer matrix
Energy transfer
Conformations

Keywords

  • Fret
  • Hybrid materials
  • Polymers
  • Proteins
  • Stress detection

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Medicine(all)

Cite this

Mechanical nanosensor based on fret within a thermosome : Damage-reporting polymeric materials. / Bruns, Nico; Pustelny, Katarzyna; Bergeron, Lisa M.; Whitehead, Timothy A.; Clark, Douglas S.

In: Angewandte Chemie - International Edition, Vol. 48, No. 31, 20.07.2009, p. 5666-5669.

Research output: Research - peer-reviewArticle

Bruns, Nico ; Pustelny, Katarzyna ; Bergeron, Lisa M. ; Whitehead, Timothy A. ; Clark, Douglas S./ Mechanical nanosensor based on fret within a thermosome : Damage-reporting polymeric materials. In: Angewandte Chemie - International Edition. 2009 ; Vol. 48, No. 31. pp. 5666-5669
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