Metabolic engineering to increase the corn seed storage lipid quantity and change its compositional quality

Hussien Alameldin, Ali Izadi-Darbandi, Scott A. Smith, Venkatesh Balan, A. Daniel Jones, Gul Ebru Orhun, Mariam Sticklen

Research output: Contribution to journalArticle

Abstract

Given limited global food supplies and the fact that the global population is expected to double by 2050, there is an urgent need for the development of high-calorie foods, including culinary oils. The seeds of oil crops contain high-energy density oil composed of triacylglycerides (TAGs) at up to 80% by dry mass. However, maize (Zea mays L.) seeds are relatively poor in calorie and nutritional values. Therefore, in this report, we address this constraint via metabolic engineering to improve maize seed lipids including TAG and seed TAG nutritional values by overexpression of three major genes, including: (i) the Arabidopsis thaliana (L.) Heynh. diacylglycerol acyltransferase 1 (AtDGAT1), a gene that catalyzes the TAG biosynthesis final step prior to packaging of TAGs into oil bodies; (ii) the transcription factor WRINKLED 1 (WRI1), which promotes the regulation of the expression of genes involved in fatty acid biosynthesis; and (i) the A. thaliana oleosin (AtOleosin) gene, a gene coding for a protein that protects TAGs from degradation. The overexpression of the above three genes, and probably certain unintentional in vitro culture genetic variabilities, resulted in 117% increase of seed TAGs and 25% increase in total oil contents when compared with the wild-type control corn seeds. In addition, the above genetic modifications led to major shifts in the fatty acid profiles in favor of human health.

Original languageEnglish (US)
Pages (from-to)1854-1864
Number of pages11
JournalCrop Science
Volume57
Issue number4
DOIs
StatePublished - 2017

Profile

seeds
corn
genes
metabolic engineering
Arabidopsis thaliana
nutritive value
biosynthesis
oils
lipids
oleosin
diacylglycerol acyltransferase
oilseed crops
lipid bodies
seed storage
energy density
gene expression regulation
major genes
genetic engineering
in vitro culture
packaging

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Metabolic engineering to increase the corn seed storage lipid quantity and change its compositional quality. / Alameldin, Hussien; Izadi-Darbandi, Ali; Smith, Scott A.; Balan, Venkatesh; Jones, A. Daniel; Ebru Orhun, Gul; Sticklen, Mariam.

In: Crop Science, Vol. 57, No. 4, 2017, p. 1854-1864.

Research output: Contribution to journalArticle

Alameldin, Hussien; Izadi-Darbandi, Ali; Smith, Scott A.; Balan, Venkatesh; Jones, A. Daniel; Ebru Orhun, Gul; Sticklen, Mariam / Metabolic engineering to increase the corn seed storage lipid quantity and change its compositional quality.

In: Crop Science, Vol. 57, No. 4, 2017, p. 1854-1864.

Research output: Contribution to journalArticle

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