Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris

Samantha Austin, Wayne S. Kontur, Arne Ulbrich, J. Zachary Oshlag, Weiping Zhang, Alan Higbee, Yaoping Zhang, Joshua J. Coon, David B. Hodge, Timothy J. Donohue, Daniel R. Noguera

    Research output: Contribution to journalArticle

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    Abstract

    Lignocellulosic biomass hydrolysates hold great potential as a feedstock for microbial biofuel production, due to their high concentration of fermentable sugars. Present at lower concentrations are a suite of aromatic compounds that can inhibit fermentation by biofuel-producing microbes. We have developed a microbial-mediated strategy for removing these aromatic compounds, using the purple nonsulfur bacterium Rhodopseudomonas palustris. When grown photoheterotrophically in an anaerobic environment, R. palustris removes most of the aromatics from ammonia fiber expansion (AFEX) treated corn stover hydrolysate (ACSH), while leaving the sugars mostly intact. We show that R. palustris can metabolize a host of aromatic substrates in ACSH that have either been previously described as unable to support growth, such as methoxylated aromatics, and those that have not yet been tested, such as aromatic amides. Removing the aromatics from ACSH with R. palustris, allowed growth of a second microbe that could not grow in the untreated ACSH. By using defined mutants, we show that most of these aromatic compounds are metabolized by the benzoyl-CoA pathway. We also show that loss of enzymes in the benzoyl-CoA pathway prevents total degradation of the aromatics in the hydrolysate, and instead allows for biological transformation of this suite of aromatics into selected aromatic compounds potentially recoverable as an additional bioproduct.

    Original languageEnglish (US)
    Pages (from-to)8914-8922
    Number of pages9
    JournalEnvironmental Science and Technology
    Volume49
    Issue number14
    DOIs
    StatePublished - Jul 21 2015

    Profile

    Behavioral Medicine
    maize
    Erythrasma
    biofuel
    sugar
    Methacholine Compounds
    Abnormal Erythrocytes
    Saimiriine herpesvirus 2
    Panthera
    4-Aminobutyrate Transaminase
    Proteobacteria
    Metabolism
    Fermentation
    Feedstocks
    Substrates
    fermentation
    ammonia
    metabolism
    enzyme
    substrate

    ASJC Scopus subject areas

    • Chemistry(all)
    • Environmental Chemistry

    Cite this

    Austin, S., Kontur, W. S., Ulbrich, A., Oshlag, J. Z., Zhang, W., Higbee, A., ... Noguera, D. R. (2015). Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris. Environmental Science and Technology, 49(14), 8914-8922. DOI: 10.1021/acs.est.5b02062

    Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris. / Austin, Samantha; Kontur, Wayne S.; Ulbrich, Arne; Oshlag, J. Zachary; Zhang, Weiping; Higbee, Alan; Zhang, Yaoping; Coon, Joshua J.; Hodge, David B.; Donohue, Timothy J.; Noguera, Daniel R.

    In: Environmental Science and Technology, Vol. 49, No. 14, 21.07.2015, p. 8914-8922.

    Research output: Contribution to journalArticle

    Austin, S, Kontur, WS, Ulbrich, A, Oshlag, JZ, Zhang, W, Higbee, A, Zhang, Y, Coon, JJ, Hodge, DB, Donohue, TJ & Noguera, DR 2015, 'Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris' Environmental Science and Technology, vol 49, no. 14, pp. 8914-8922. DOI: 10.1021/acs.est.5b02062
    Austin S, Kontur WS, Ulbrich A, Oshlag JZ, Zhang W, Higbee A et al. Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris. Environmental Science and Technology. 2015 Jul 21;49(14):8914-8922. Available from, DOI: 10.1021/acs.est.5b02062

    Austin, Samantha; Kontur, Wayne S.; Ulbrich, Arne; Oshlag, J. Zachary; Zhang, Weiping; Higbee, Alan; Zhang, Yaoping; Coon, Joshua J.; Hodge, David B.; Donohue, Timothy J.; Noguera, Daniel R. / Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris.

    In: Environmental Science and Technology, Vol. 49, No. 14, 21.07.2015, p. 8914-8922.

    Research output: Contribution to journalArticle

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    abstract = "Lignocellulosic biomass hydrolysates hold great potential as a feedstock for microbial biofuel production, due to their high concentration of fermentable sugars. Present at lower concentrations are a suite of aromatic compounds that can inhibit fermentation by biofuel-producing microbes. We have developed a microbial-mediated strategy for removing these aromatic compounds, using the purple nonsulfur bacterium Rhodopseudomonas palustris. When grown photoheterotrophically in an anaerobic environment, R. palustris removes most of the aromatics from ammonia fiber expansion (AFEX) treated corn stover hydrolysate (ACSH), while leaving the sugars mostly intact. We show that R. palustris can metabolize a host of aromatic substrates in ACSH that have either been previously described as unable to support growth, such as methoxylated aromatics, and those that have not yet been tested, such as aromatic amides. Removing the aromatics from ACSH with R. palustris, allowed growth of a second microbe that could not grow in the untreated ACSH. By using defined mutants, we show that most of these aromatic compounds are metabolized by the benzoyl-CoA pathway. We also show that loss of enzymes in the benzoyl-CoA pathway prevents total degradation of the aromatics in the hydrolysate, and instead allows for biological transformation of this suite of aromatics into selected aromatic compounds potentially recoverable as an additional bioproduct.",
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