Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris

Samantha Austin, Wayne S. Kontur, Arne Ulbrich, J. Zachary Oshlag, Weiping Zhang, Alan Higbee, Yaoping Zhang, Joshua J. Coon, David B. Hodge, Timothy J. Donohue, Daniel R. Noguera

Research output: Contribution to journalArticle

  • 6 Citations

Abstract

Lignocellulosic biomass hydrolysates hold great potential as a feedstock for microbial biofuel production, due to their high concentration of fermentable sugars. Present at lower concentrations are a suite of aromatic compounds that can inhibit fermentation by biofuel-producing microbes. We have developed a microbial-mediated strategy for removing these aromatic compounds, using the purple nonsulfur bacterium Rhodopseudomonas palustris. When grown photoheterotrophically in an anaerobic environment, R. palustris removes most of the aromatics from ammonia fiber expansion (AFEX) treated corn stover hydrolysate (ACSH), while leaving the sugars mostly intact. We show that R. palustris can metabolize a host of aromatic substrates in ACSH that have either been previously described as unable to support growth, such as methoxylated aromatics, and those that have not yet been tested, such as aromatic amides. Removing the aromatics from ACSH with R. palustris, allowed growth of a second microbe that could not grow in the untreated ACSH. By using defined mutants, we show that most of these aromatic compounds are metabolized by the benzoyl-CoA pathway. We also show that loss of enzymes in the benzoyl-CoA pathway prevents total degradation of the aromatics in the hydrolysate, and instead allows for biological transformation of this suite of aromatics into selected aromatic compounds potentially recoverable as an additional bioproduct.

LanguageEnglish (US)
Pages8914-8922
Number of pages9
JournalEnvironmental Science and Technology
Volume49
Issue number14
DOIs
StatePublished - Jul 21 2015

Profile

Aromatic compounds
Metabolism
metabolism
maize
Biofuels
biofuel
Sugars
sugar
Ammonia
Amides
Fermentation
Feedstocks
fermentation
Biomass
ammonia
enzyme
substrate
Degradation
degradation
bacterium

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Austin, S., Kontur, W. S., Ulbrich, A., Oshlag, J. Z., Zhang, W., Higbee, A., ... Noguera, D. R. (2015). Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris. Environmental Science and Technology, 49(14), 8914-8922. DOI: 10.1021/acs.est.5b02062

Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris. / Austin, Samantha; Kontur, Wayne S.; Ulbrich, Arne; Oshlag, J. Zachary; Zhang, Weiping; Higbee, Alan; Zhang, Yaoping; Coon, Joshua J.; Hodge, David B.; Donohue, Timothy J.; Noguera, Daniel R.

In: Environmental Science and Technology, Vol. 49, No. 14, 21.07.2015, p. 8914-8922.

Research output: Contribution to journalArticle

Austin, S, Kontur, WS, Ulbrich, A, Oshlag, JZ, Zhang, W, Higbee, A, Zhang, Y, Coon, JJ, Hodge, DB, Donohue, TJ & Noguera, DR 2015, 'Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris' Environmental Science and Technology, vol 49, no. 14, pp. 8914-8922. DOI: 10.1021/acs.est.5b02062
Austin S, Kontur WS, Ulbrich A, Oshlag JZ, Zhang W, Higbee A et al. Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris. Environmental Science and Technology. 2015 Jul 21;49(14):8914-8922. Available from, DOI: 10.1021/acs.est.5b02062
Austin, Samantha ; Kontur, Wayne S. ; Ulbrich, Arne ; Oshlag, J. Zachary ; Zhang, Weiping ; Higbee, Alan ; Zhang, Yaoping ; Coon, Joshua J. ; Hodge, David B. ; Donohue, Timothy J. ; Noguera, Daniel R./ Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris. In: Environmental Science and Technology. 2015 ; Vol. 49, No. 14. pp. 8914-8922
@article{95df0f85be674dab9236299806896b5a,
title = "Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris",
abstract = "Lignocellulosic biomass hydrolysates hold great potential as a feedstock for microbial biofuel production, due to their high concentration of fermentable sugars. Present at lower concentrations are a suite of aromatic compounds that can inhibit fermentation by biofuel-producing microbes. We have developed a microbial-mediated strategy for removing these aromatic compounds, using the purple nonsulfur bacterium Rhodopseudomonas palustris. When grown photoheterotrophically in an anaerobic environment, R. palustris removes most of the aromatics from ammonia fiber expansion (AFEX) treated corn stover hydrolysate (ACSH), while leaving the sugars mostly intact. We show that R. palustris can metabolize a host of aromatic substrates in ACSH that have either been previously described as unable to support growth, such as methoxylated aromatics, and those that have not yet been tested, such as aromatic amides. Removing the aromatics from ACSH with R. palustris, allowed growth of a second microbe that could not grow in the untreated ACSH. By using defined mutants, we show that most of these aromatic compounds are metabolized by the benzoyl-CoA pathway. We also show that loss of enzymes in the benzoyl-CoA pathway prevents total degradation of the aromatics in the hydrolysate, and instead allows for biological transformation of this suite of aromatics into selected aromatic compounds potentially recoverable as an additional bioproduct.",
author = "Samantha Austin and Kontur, {Wayne S.} and Arne Ulbrich and Oshlag, {J. Zachary} and Weiping Zhang and Alan Higbee and Yaoping Zhang and Coon, {Joshua J.} and Hodge, {David B.} and Donohue, {Timothy J.} and Noguera, {Daniel R.}",
year = "2015",
month = "7",
day = "21",
doi = "10.1021/acs.est.5b02062",
language = "English (US)",
volume = "49",
pages = "8914--8922",
journal = "Environmental Science & Technology",
issn = "0013-936X",
publisher = "American Chemical Society",
number = "14",

}

TY - JOUR

T1 - Metabolism of Multiple Aromatic Compounds in Corn Stover Hydrolysate by Rhodopseudomonas palustris

AU - Austin,Samantha

AU - Kontur,Wayne S.

AU - Ulbrich,Arne

AU - Oshlag,J. Zachary

AU - Zhang,Weiping

AU - Higbee,Alan

AU - Zhang,Yaoping

AU - Coon,Joshua J.

AU - Hodge,David B.

AU - Donohue,Timothy J.

AU - Noguera,Daniel R.

PY - 2015/7/21

Y1 - 2015/7/21

N2 - Lignocellulosic biomass hydrolysates hold great potential as a feedstock for microbial biofuel production, due to their high concentration of fermentable sugars. Present at lower concentrations are a suite of aromatic compounds that can inhibit fermentation by biofuel-producing microbes. We have developed a microbial-mediated strategy for removing these aromatic compounds, using the purple nonsulfur bacterium Rhodopseudomonas palustris. When grown photoheterotrophically in an anaerobic environment, R. palustris removes most of the aromatics from ammonia fiber expansion (AFEX) treated corn stover hydrolysate (ACSH), while leaving the sugars mostly intact. We show that R. palustris can metabolize a host of aromatic substrates in ACSH that have either been previously described as unable to support growth, such as methoxylated aromatics, and those that have not yet been tested, such as aromatic amides. Removing the aromatics from ACSH with R. palustris, allowed growth of a second microbe that could not grow in the untreated ACSH. By using defined mutants, we show that most of these aromatic compounds are metabolized by the benzoyl-CoA pathway. We also show that loss of enzymes in the benzoyl-CoA pathway prevents total degradation of the aromatics in the hydrolysate, and instead allows for biological transformation of this suite of aromatics into selected aromatic compounds potentially recoverable as an additional bioproduct.

AB - Lignocellulosic biomass hydrolysates hold great potential as a feedstock for microbial biofuel production, due to their high concentration of fermentable sugars. Present at lower concentrations are a suite of aromatic compounds that can inhibit fermentation by biofuel-producing microbes. We have developed a microbial-mediated strategy for removing these aromatic compounds, using the purple nonsulfur bacterium Rhodopseudomonas palustris. When grown photoheterotrophically in an anaerobic environment, R. palustris removes most of the aromatics from ammonia fiber expansion (AFEX) treated corn stover hydrolysate (ACSH), while leaving the sugars mostly intact. We show that R. palustris can metabolize a host of aromatic substrates in ACSH that have either been previously described as unable to support growth, such as methoxylated aromatics, and those that have not yet been tested, such as aromatic amides. Removing the aromatics from ACSH with R. palustris, allowed growth of a second microbe that could not grow in the untreated ACSH. By using defined mutants, we show that most of these aromatic compounds are metabolized by the benzoyl-CoA pathway. We also show that loss of enzymes in the benzoyl-CoA pathway prevents total degradation of the aromatics in the hydrolysate, and instead allows for biological transformation of this suite of aromatics into selected aromatic compounds potentially recoverable as an additional bioproduct.

UR - http://www.scopus.com/inward/record.url?scp=84937458566&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937458566&partnerID=8YFLogxK

U2 - 10.1021/acs.est.5b02062

DO - 10.1021/acs.est.5b02062

M3 - Article

VL - 49

SP - 8914

EP - 8922

JO - Environmental Science & Technology

T2 - Environmental Science & Technology

JF - Environmental Science & Technology

SN - 0013-936X

IS - 14

ER -