Microbial lipid production from AFEX™ pretreated corn stover

Ya Ping Xue, Mingjie Jin, Andrea Orjuela, Patricia J. Slininger, Bruce S. Dien, Bruce E. Dale, Venkatesh Balan

    Research output: Contribution to journalArticle

    • 5 Citations

    Abstract

    Lipids having high carbon to heteroatom ratios can be upgraded to bio-diesel and jet fuels which are more advanced drop-in fuels compared to ethanol. The present study investigated microbial lipid production from Ammonia Fiber Expansion (AFEX) pretreated and hydrolyzed corn stover (CS) using an oleaginous yeast strain Lipomyces tetrasporus NRRL Y-11562. Process conditions were optimized for carbon to nitrogen ratio of fermentation medium, fermentation temperature and pH, and solid loading of AFEX-CS. The inhibitory effect of AFEX degradation products on lipid fermentation was also investigated. Both separate hydrolysis and fermentation (SHF) and Rapid Bioconversion with Integrated recycle Technology (RaBIT) processes were used for lipid production. From 1 kg AFEX-CS, 36.7 g lipids were produced via SHF at a titer of 8.4 g L-1 with a yield of 0.08 g g-1 consumed sugar. A yeast meal stream (97.9 g) was also generated. L. tetrasporus NRRL Y-11562 grew better in AFEX-CS hydrolysate, but produced fewer lipids compared to synthetic medium. Minimal washing of AFEX-CS improved the lipid yield and titer to 0.10 g g-1 consumed sugar and 10.7 g L-1, respectively. RaBIT on washed AFEX-CS generated a similar amount of lipids compared to SHF with 35% lower enzyme loading. Economic analysis does not favor lignocellulosic lipid production with current lipid yields. This journal is

    Original languageEnglish (US)
    Pages (from-to)28725-28734
    Number of pages10
    JournalRSC Advances
    Volume5
    Issue number36
    DOIs
    StatePublished - 2015

    Profile

    Lipids
    Ammonia
    Fibers
    Abnormal Erythrocytes
    Saimiriine herpesvirus 2
    Fermentation
    Hydrolysis
    Bioconversion
    Sugars
    Yeast
    Carbon
    Acyclic Acids
    HLA Antigens
    Jet fuel
    Economic analysis
    Diesel fuels
    Biodiesel
    Washing
    Ethanol
    Enzymes

    ASJC Scopus subject areas

    • Chemical Engineering(all)
    • Chemistry(all)

    Cite this

    Xue, Y. P., Jin, M., Orjuela, A., Slininger, P. J., Dien, B. S., Dale, B. E., & Balan, V. (2015). Microbial lipid production from AFEX™ pretreated corn stover. RSC Advances, 5(36), 28725-28734. DOI: 10.1039/c5ra01134e

    Microbial lipid production from AFEX™ pretreated corn stover. / Xue, Ya Ping; Jin, Mingjie; Orjuela, Andrea; Slininger, Patricia J.; Dien, Bruce S.; Dale, Bruce E.; Balan, Venkatesh.

    In: RSC Advances, Vol. 5, No. 36, 2015, p. 28725-28734.

    Research output: Contribution to journalArticle

    Xue, YP, Jin, M, Orjuela, A, Slininger, PJ, Dien, BS, Dale, BE & Balan, V 2015, 'Microbial lipid production from AFEX™ pretreated corn stover' RSC Advances, vol 5, no. 36, pp. 28725-28734. DOI: 10.1039/c5ra01134e
    Xue YP, Jin M, Orjuela A, Slininger PJ, Dien BS, Dale BE et al. Microbial lipid production from AFEX™ pretreated corn stover. RSC Advances. 2015;5(36):28725-28734. Available from, DOI: 10.1039/c5ra01134e

    Xue, Ya Ping; Jin, Mingjie; Orjuela, Andrea; Slininger, Patricia J.; Dien, Bruce S.; Dale, Bruce E.; Balan, Venkatesh / Microbial lipid production from AFEX™ pretreated corn stover.

    In: RSC Advances, Vol. 5, No. 36, 2015, p. 28725-28734.

    Research output: Contribution to journalArticle

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    abstract = "Lipids having high carbon to heteroatom ratios can be upgraded to bio-diesel and jet fuels which are more advanced drop-in fuels compared to ethanol. The present study investigated microbial lipid production from Ammonia Fiber Expansion (AFEX) pretreated and hydrolyzed corn stover (CS) using an oleaginous yeast strain Lipomyces tetrasporus NRRL Y-11562. Process conditions were optimized for carbon to nitrogen ratio of fermentation medium, fermentation temperature and pH, and solid loading of AFEX-CS. The inhibitory effect of AFEX degradation products on lipid fermentation was also investigated. Both separate hydrolysis and fermentation (SHF) and Rapid Bioconversion with Integrated recycle Technology (RaBIT) processes were used for lipid production. From 1 kg AFEX-CS, 36.7 g lipids were produced via SHF at a titer of 8.4 g L-1 with a yield of 0.08 g g-1 consumed sugar. A yeast meal stream (97.9 g) was also generated. L. tetrasporus NRRL Y-11562 grew better in AFEX-CS hydrolysate, but produced fewer lipids compared to synthetic medium. Minimal washing of AFEX-CS improved the lipid yield and titer to 0.10 g g-1 consumed sugar and 10.7 g L-1, respectively. RaBIT on washed AFEX-CS generated a similar amount of lipids compared to SHF with 35% lower enzyme loading. Economic analysis does not favor lignocellulosic lipid production with current lipid yields. This journal is",
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