Microbial lipid production from AFEX™ pretreated corn stover

Ya Ping Xue, Mingjie Jin, Andrea Orjuela, Patricia J. Slininger, Bruce S. Dien, Bruce E. Dale, Venkatesh Balan

Research output: Contribution to journalArticle

  • 7 Citations

Abstract

Lipids having high carbon to heteroatom ratios can be upgraded to bio-diesel and jet fuels which are more advanced drop-in fuels compared to ethanol. The present study investigated microbial lipid production from Ammonia Fiber Expansion (AFEX) pretreated and hydrolyzed corn stover (CS) using an oleaginous yeast strain Lipomyces tetrasporus NRRL Y-11562. Process conditions were optimized for carbon to nitrogen ratio of fermentation medium, fermentation temperature and pH, and solid loading of AFEX-CS. The inhibitory effect of AFEX degradation products on lipid fermentation was also investigated. Both separate hydrolysis and fermentation (SHF) and Rapid Bioconversion with Integrated recycle Technology (RaBIT) processes were used for lipid production. From 1 kg AFEX-CS, 36.7 g lipids were produced via SHF at a titer of 8.4 g L-1 with a yield of 0.08 g g-1 consumed sugar. A yeast meal stream (97.9 g) was also generated. L. tetrasporus NRRL Y-11562 grew better in AFEX-CS hydrolysate, but produced fewer lipids compared to synthetic medium. Minimal washing of AFEX-CS improved the lipid yield and titer to 0.10 g g-1 consumed sugar and 10.7 g L-1, respectively. RaBIT on washed AFEX-CS generated a similar amount of lipids compared to SHF with 35% lower enzyme loading. Economic analysis does not favor lignocellulosic lipid production with current lipid yields. This journal is

LanguageEnglish (US)
Pages28725-28734
Number of pages10
JournalRSC Advances
Volume5
Issue number36
DOIs
StatePublished - 2015

Profile

Ammonia
Lipids
Fibers
Fermentation
Hydrolysis
Bioconversion
Sugars
Yeast
Carbon
Jet fuel
Economic analysis
Diesel fuels
Biodiesel
Washing
Ethanol
Nitrogen
Enzymes
Degradation

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Xue, Y. P., Jin, M., Orjuela, A., Slininger, P. J., Dien, B. S., Dale, B. E., & Balan, V. (2015). Microbial lipid production from AFEX™ pretreated corn stover. RSC Advances, 5(36), 28725-28734. DOI: 10.1039/c5ra01134e

Microbial lipid production from AFEX™ pretreated corn stover. / Xue, Ya Ping; Jin, Mingjie; Orjuela, Andrea; Slininger, Patricia J.; Dien, Bruce S.; Dale, Bruce E.; Balan, Venkatesh.

In: RSC Advances, Vol. 5, No. 36, 2015, p. 28725-28734.

Research output: Contribution to journalArticle

Xue, YP, Jin, M, Orjuela, A, Slininger, PJ, Dien, BS, Dale, BE & Balan, V 2015, 'Microbial lipid production from AFEX™ pretreated corn stover' RSC Advances, vol 5, no. 36, pp. 28725-28734. DOI: 10.1039/c5ra01134e
Xue YP, Jin M, Orjuela A, Slininger PJ, Dien BS, Dale BE et al. Microbial lipid production from AFEX™ pretreated corn stover. RSC Advances. 2015;5(36):28725-28734. Available from, DOI: 10.1039/c5ra01134e
Xue, Ya Ping ; Jin, Mingjie ; Orjuela, Andrea ; Slininger, Patricia J. ; Dien, Bruce S. ; Dale, Bruce E. ; Balan, Venkatesh. / Microbial lipid production from AFEX™ pretreated corn stover. In: RSC Advances. 2015 ; Vol. 5, No. 36. pp. 28725-28734
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