Nanostructured biosensor for measuring neuropathy target esterase activity

Neeraj Kohli, Devesh Srivastava, Jun Sun, Rudy J. Richardson, Ilsoon Lee, Robert M. Worden

Research output: Contribution to journalArticle

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Abstract

Neuropathy target esterase (NTE) is a membrane protein found in human neurons and other cells, including lymphocytes. Binding of certain organophosphorus (OP) compounds to NTE is believed to cause OP-induced delayed neuropathy (OPIDN), a type of paralysis for which there is no effective treatment. Mutations in NTE have also been linked with serious neurological diseases, such as motor neuron disease. This paper describes development of the first nanostructured biosensor interface containing a catalytically active fragment of NTE known as NEST. The biosensor was fabricated using the layer-by-layer assembly approach, by immobilizing a layer of NEST on top of multilayers consisting of a polyelectrolyte (poly-L-lysine) and an enzyme (tyrosinase). The biosensor has a response time on the order of seconds and gives a concentration-dependent decrease in sensor output in response to a known NEST (and NTE) inhibitor. Potential applications of the biosensor include screening OP compounds for NTE inhibition and investigating the enzymology of wild-type and mutant forms of NTE. Although the development of a NEST biosensor was the primary purpose of this study, we found that the approach developed for NEST could also be extended to measure the activity of other esterases involved in neural processes, such as acetyl-cholinesterase (AChE) and butyrylcholinesterase (BChE). On the basis of measured sensitivities, phenyl valerate was the preferred substrate for NEST and BChE, whereas phenyl acetate was better for AChE.

LanguageEnglish (US)
Pages5196-5203
Number of pages8
JournalAnalytical Chemistry
Volume79
Issue number14
DOIs
StatePublished - Jul 15 2007

Profile

Biosensors
Organophosphorus Compounds
Butyrylcholinesterase
Cholinesterases
Neurons
Lymphocytes
Monophenol Monooxygenase
Esterases
neurotoxic esterase
Polyelectrolytes
Lysine
Screening
Multilayers
Membrane Proteins
Sensors
Substrates
Enzymes

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Nanostructured biosensor for measuring neuropathy target esterase activity. / Kohli, Neeraj; Srivastava, Devesh; Sun, Jun; Richardson, Rudy J.; Lee, Ilsoon; Worden, Robert M.

In: Analytical Chemistry, Vol. 79, No. 14, 15.07.2007, p. 5196-5203.

Research output: Contribution to journalArticle

Kohli, N, Srivastava, D, Sun, J, Richardson, RJ, Lee, I & Worden, RM 2007, 'Nanostructured biosensor for measuring neuropathy target esterase activity' Analytical Chemistry, vol. 79, no. 14, pp. 5196-5203. DOI: 10.1021/ac0701684
Kohli N, Srivastava D, Sun J, Richardson RJ, Lee I, Worden RM. Nanostructured biosensor for measuring neuropathy target esterase activity. Analytical Chemistry. 2007 Jul 15;79(14):5196-5203. Available from, DOI: 10.1021/ac0701684
Kohli, Neeraj ; Srivastava, Devesh ; Sun, Jun ; Richardson, Rudy J. ; Lee, Ilsoon ; Worden, Robert M./ Nanostructured biosensor for measuring neuropathy target esterase activity. In: Analytical Chemistry. 2007 ; Vol. 79, No. 14. pp. 5196-5203
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