Next-generation ammonia pretreatment enhances cellulosic biofuel production

Leonardo Da Costa Sousa, Mingjie Jin, Shishir P S Chundawat, Vijay Bokade, Xiaoyu Tang, Ali Azarpira, Fachuang Lu, Utku Avci, James Humpula, Nirmal Uppugundla, Christa Gunawan, Sivakumar Pattathil, Albert M. Cheh, Ninad Kothari, Rajeev Kumar, John Ralph, Michael G. Hahn, Charles E. Wyman, Seema Singh, Blake A. Simmons & 2 others Bruce E. Dale, Venkatesh Balan

Research output: Contribution to journalArticle

  • 44 Citations

Abstract

A new liquid ammonia pretreatment methodology called Extractive Ammonia (EA) was developed to simultaneously convert native crystalline cellulose Iβ (CI) to a highly digestible cellulose IIII (CIII) allomorph and selectively extract up to ∼45% of the lignin from lignocellulosic biomass with near-quantitative retention of all polysaccharides. EA pretreated corn stover yielded a higher fermentable sugar yield compared to the older Ammonia Fiber Expansion (AFEX) process while using 60% lower enzyme loading. The EA process preserves extracted lignin functionalities, offering the potential to co-produce lignin-derived fuels and chemicals in the biorefinery. The single-stage EA fractionation process achieves high biofuel yields (18.2 kg ethanol per 100 kg untreated corn stover, dry weight basis), comparable to those achieved using ionic liquid pretreatments. The EA process achieves these ethanol yields at industrially-relevant conditions using low enzyme loading (7.5 mg protein per g glucan) and high solids loading (8% glucan, w/v).

LanguageEnglish (US)
Pages1215-1223
Number of pages9
JournalEnergy and Environmental Science
Volume9
Issue number4
DOIs
StatePublished - Apr 1 2016

Profile

Biofuels
Ammonia
biofuel
ammonia
Lignin
lignin
Glucans
Cellulose
cellulose
ethanol
Ethanol
Enzymes
maize
enzyme
Ionic Liquids
Polysaccharides
Fractionation
polysaccharide
Ionic liquids
Sugars

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Environmental Chemistry
  • Pollution
  • Nuclear Energy and Engineering

Cite this

Da Costa Sousa, L., Jin, M., Chundawat, S. P. S., Bokade, V., Tang, X., Azarpira, A., ... Balan, V. (2016). Next-generation ammonia pretreatment enhances cellulosic biofuel production. Energy and Environmental Science, 9(4), 1215-1223. DOI: 10.1039/c5ee03051j

Next-generation ammonia pretreatment enhances cellulosic biofuel production. / Da Costa Sousa, Leonardo; Jin, Mingjie; Chundawat, Shishir P S; Bokade, Vijay; Tang, Xiaoyu; Azarpira, Ali; Lu, Fachuang; Avci, Utku; Humpula, James; Uppugundla, Nirmal; Gunawan, Christa; Pattathil, Sivakumar; Cheh, Albert M.; Kothari, Ninad; Kumar, Rajeev; Ralph, John; Hahn, Michael G.; Wyman, Charles E.; Singh, Seema; Simmons, Blake A.; Dale, Bruce E.; Balan, Venkatesh.

In: Energy and Environmental Science, Vol. 9, No. 4, 01.04.2016, p. 1215-1223.

Research output: Contribution to journalArticle

Da Costa Sousa, L, Jin, M, Chundawat, SPS, Bokade, V, Tang, X, Azarpira, A, Lu, F, Avci, U, Humpula, J, Uppugundla, N, Gunawan, C, Pattathil, S, Cheh, AM, Kothari, N, Kumar, R, Ralph, J, Hahn, MG, Wyman, CE, Singh, S, Simmons, BA, Dale, BE & Balan, V 2016, 'Next-generation ammonia pretreatment enhances cellulosic biofuel production' Energy and Environmental Science, vol 9, no. 4, pp. 1215-1223. DOI: 10.1039/c5ee03051j
Da Costa Sousa L, Jin M, Chundawat SPS, Bokade V, Tang X, Azarpira A et al. Next-generation ammonia pretreatment enhances cellulosic biofuel production. Energy and Environmental Science. 2016 Apr 1;9(4):1215-1223. Available from, DOI: 10.1039/c5ee03051j
Da Costa Sousa, Leonardo ; Jin, Mingjie ; Chundawat, Shishir P S ; Bokade, Vijay ; Tang, Xiaoyu ; Azarpira, Ali ; Lu, Fachuang ; Avci, Utku ; Humpula, James ; Uppugundla, Nirmal ; Gunawan, Christa ; Pattathil, Sivakumar ; Cheh, Albert M. ; Kothari, Ninad ; Kumar, Rajeev ; Ralph, John ; Hahn, Michael G. ; Wyman, Charles E. ; Singh, Seema ; Simmons, Blake A. ; Dale, Bruce E. ; Balan, Venkatesh. / Next-generation ammonia pretreatment enhances cellulosic biofuel production. In: Energy and Environmental Science. 2016 ; Vol. 9, No. 4. pp. 1215-1223
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