Next-generation ammonia pretreatment enhances cellulosic biofuel production

Leonardo Da Costa Sousa, Mingjie Jin, Shishir P S Chundawat, Vijay Bokade, Xiaoyu Tang, Ali Azarpira, Fachuang Lu, Utku Avci, James Humpula, Nirmal Uppugundla, Christa Gunawan, Sivakumar Pattathil, Albert M. Cheh, Ninad Kothari, Rajeev Kumar, John Ralph, Michael G. Hahn, Charles E. Wyman, Seema Singh, Blake A. Simmons & 2 others Bruce E. Dale, Venkatesh Balan

    Research output: Contribution to journalArticle

    • 25 Citations

    Abstract

    A new liquid ammonia pretreatment methodology called Extractive Ammonia (EA) was developed to simultaneously convert native crystalline cellulose Iβ (CI) to a highly digestible cellulose IIII (CIII) allomorph and selectively extract up to ∼45% of the lignin from lignocellulosic biomass with near-quantitative retention of all polysaccharides. EA pretreated corn stover yielded a higher fermentable sugar yield compared to the older Ammonia Fiber Expansion (AFEX) process while using 60% lower enzyme loading. The EA process preserves extracted lignin functionalities, offering the potential to co-produce lignin-derived fuels and chemicals in the biorefinery. The single-stage EA fractionation process achieves high biofuel yields (18.2 kg ethanol per 100 kg untreated corn stover, dry weight basis), comparable to those achieved using ionic liquid pretreatments. The EA process achieves these ethanol yields at industrially-relevant conditions using low enzyme loading (7.5 mg protein per g glucan) and high solids loading (8% glucan, w/v).

    Original languageEnglish (US)
    Pages (from-to)1215-1223
    Number of pages9
    JournalEnergy and Environmental Science
    Volume9
    Issue number4
    DOIs
    StatePublished - Apr 1 2016

    Profile

    ammonia
    Ammonia
    lignin
    Lignin
    biofuel
    cellulose
    ethanol
    maize
    enzyme
    liquid
    Biofuels
    Cellulose
    Ethanol
    Enzymes
    polysaccharide
    sugar
    fractionation
    protein
    methodology
    biomass

    ASJC Scopus subject areas

    • Renewable Energy, Sustainability and the Environment
    • Environmental Chemistry
    • Pollution
    • Nuclear Energy and Engineering

    Cite this

    Da Costa Sousa, L., Jin, M., Chundawat, S. P. S., Bokade, V., Tang, X., Azarpira, A., ... Balan, V. (2016). Next-generation ammonia pretreatment enhances cellulosic biofuel production. Energy and Environmental Science, 9(4), 1215-1223. DOI: 10.1039/c5ee03051j

    Next-generation ammonia pretreatment enhances cellulosic biofuel production. / Da Costa Sousa, Leonardo; Jin, Mingjie; Chundawat, Shishir P S; Bokade, Vijay; Tang, Xiaoyu; Azarpira, Ali; Lu, Fachuang; Avci, Utku; Humpula, James; Uppugundla, Nirmal; Gunawan, Christa; Pattathil, Sivakumar; Cheh, Albert M.; Kothari, Ninad; Kumar, Rajeev; Ralph, John; Hahn, Michael G.; Wyman, Charles E.; Singh, Seema; Simmons, Blake A.; Dale, Bruce E.; Balan, Venkatesh.

    In: Energy and Environmental Science, Vol. 9, No. 4, 01.04.2016, p. 1215-1223.

    Research output: Contribution to journalArticle

    Da Costa Sousa, L, Jin, M, Chundawat, SPS, Bokade, V, Tang, X, Azarpira, A, Lu, F, Avci, U, Humpula, J, Uppugundla, N, Gunawan, C, Pattathil, S, Cheh, AM, Kothari, N, Kumar, R, Ralph, J, Hahn, MG, Wyman, CE, Singh, S, Simmons, BA, Dale, BE & Balan, V 2016, 'Next-generation ammonia pretreatment enhances cellulosic biofuel production' Energy and Environmental Science, vol 9, no. 4, pp. 1215-1223. DOI: 10.1039/c5ee03051j
    Da Costa Sousa L, Jin M, Chundawat SPS, Bokade V, Tang X, Azarpira A et al. Next-generation ammonia pretreatment enhances cellulosic biofuel production. Energy and Environmental Science. 2016 Apr 1;9(4):1215-1223. Available from, DOI: 10.1039/c5ee03051j

    Da Costa Sousa, Leonardo; Jin, Mingjie; Chundawat, Shishir P S; Bokade, Vijay; Tang, Xiaoyu; Azarpira, Ali; Lu, Fachuang; Avci, Utku; Humpula, James; Uppugundla, Nirmal; Gunawan, Christa; Pattathil, Sivakumar; Cheh, Albert M.; Kothari, Ninad; Kumar, Rajeev; Ralph, John; Hahn, Michael G.; Wyman, Charles E.; Singh, Seema; Simmons, Blake A.; Dale, Bruce E.; Balan, Venkatesh / Next-generation ammonia pretreatment enhances cellulosic biofuel production.

    In: Energy and Environmental Science, Vol. 9, No. 4, 01.04.2016, p. 1215-1223.

    Research output: Contribution to journalArticle

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