Novel thermostable endo-xylanase cloned and expressed from bacterium Geobacillus sp. WSUCF1

Aditya Bhalla, Kenneth M. Bischoff, Nirmal Uppugundla, Venkatesh Balan, Rajesh K. Sani

    Research output: Contribution to journalArticle

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    Abstract

    A gene encoding a GH10 endo-xylanase from Geobacillus sp. WSUCF1 was cloned and expressed in Escherichia coli. Recombinant endo-xylanase (37. kDa) exhibited high specific activity of 461.0. U/mg of protein. Endo-xylanase was optimally active on birchwood xylan at 70. °C and pH 6.5. The endo-xylanase was found to be highly thermostable at 50 and 60. °C, retaining 82% and 50% of its original activity, respectively, after 60. h. High xylan conversions (92%) were obtained with oat-spelt xylan hydrolysis. Higher glucan and xylan conversions were obtained on AFEX-treated corn stover with an enzyme cocktail containing WSUCF1 endo-xylanase (71% and 47%) as compared to enzyme cocktail containing commercial fungal endo-xylanase (64% and 41%). High specific activity, active at high pH's, wide substrate specificity, and higher hydrolytic activity on recalcitrant lignocellulose, make this endo-xylanase a suitable candidate for biofuel and bioprocess industries.

    Original languageEnglish (US)
    Pages (from-to)314-318
    Number of pages5
    JournalBioresource Technology
    Volume165
    Issue numberC
    DOIs
    StatePublished - 2014

    Profile

    Geobacillus
    Xylans
    Bacteria
    enzyme
    Enzymes
    biofuel
    hydrolysis
    maize
    substrate
    bacterium
    protein
    gene
    industry
    Avena sativa
    Glucans
    Biofuels
    Substrate Specificity
    Zea mays
    Industry
    Hydrolysis

    Keywords

    • AFEX-treated corn stover
    • Biofuels
    • Endo-xylanase
    • Thermostable

    ASJC Scopus subject areas

    • Bioengineering
    • Environmental Engineering
    • Waste Management and Disposal
    • Medicine(all)

    Cite this

    Novel thermostable endo-xylanase cloned and expressed from bacterium Geobacillus sp. WSUCF1. / Bhalla, Aditya; Bischoff, Kenneth M.; Uppugundla, Nirmal; Balan, Venkatesh; Sani, Rajesh K.

    In: Bioresource Technology, Vol. 165, No. C, 2014, p. 314-318.

    Research output: Contribution to journalArticle

    Bhalla, Aditya; Bischoff, Kenneth M.; Uppugundla, Nirmal; Balan, Venkatesh; Sani, Rajesh K. / Novel thermostable endo-xylanase cloned and expressed from bacterium Geobacillus sp. WSUCF1.

    In: Bioresource Technology, Vol. 165, No. C, 2014, p. 314-318.

    Research output: Contribution to journalArticle

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    abstract = "A gene encoding a GH10 endo-xylanase from Geobacillus sp. WSUCF1 was cloned and expressed in Escherichia coli. Recombinant endo-xylanase (37. kDa) exhibited high specific activity of 461.0. U/mg of protein. Endo-xylanase was optimally active on birchwood xylan at 70. °C and pH 6.5. The endo-xylanase was found to be highly thermostable at 50 and 60. °C, retaining 82% and 50% of its original activity, respectively, after 60. h. High xylan conversions (92%) were obtained with oat-spelt xylan hydrolysis. Higher glucan and xylan conversions were obtained on AFEX-treated corn stover with an enzyme cocktail containing WSUCF1 endo-xylanase (71% and 47%) as compared to enzyme cocktail containing commercial fungal endo-xylanase (64% and 41%). High specific activity, active at high pH's, wide substrate specificity, and higher hydrolytic activity on recalcitrant lignocellulose, make this endo-xylanase a suitable candidate for biofuel and bioprocess industries.",
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    AU - Balan,Venkatesh

    AU - Sani,Rajesh K.

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