Novel thermostable endo-xylanase cloned and expressed from bacterium Geobacillus sp. WSUCF1

Aditya Bhalla, Kenneth M. Bischoff, Nirmal Uppugundla, Venkatesh Balan, Rajesh K. Sani

Research output: Contribution to journalArticle

  • 22 Citations

Abstract

A gene encoding a GH10 endo-xylanase from Geobacillus sp. WSUCF1 was cloned and expressed in Escherichia coli. Recombinant endo-xylanase (37. kDa) exhibited high specific activity of 461.0. U/mg of protein. Endo-xylanase was optimally active on birchwood xylan at 70. °C and pH 6.5. The endo-xylanase was found to be highly thermostable at 50 and 60. °C, retaining 82% and 50% of its original activity, respectively, after 60. h. High xylan conversions (92%) were obtained with oat-spelt xylan hydrolysis. Higher glucan and xylan conversions were obtained on AFEX-treated corn stover with an enzyme cocktail containing WSUCF1 endo-xylanase (71% and 47%) as compared to enzyme cocktail containing commercial fungal endo-xylanase (64% and 41%). High specific activity, active at high pH's, wide substrate specificity, and higher hydrolytic activity on recalcitrant lignocellulose, make this endo-xylanase a suitable candidate for biofuel and bioprocess industries.

LanguageEnglish (US)
Pages314-318
Number of pages5
JournalBioresource Technology
Volume165
Issue numberC
DOIs
StatePublished - 2014

Profile

Geobacillus
Xylans
Bacteria
enzyme
bacterium
biofuel
hydrolysis
Enzymes
maize
substrate
Gene encoding
protein
Glucans
Biofuels
gene
industry
Substrate Specificity
Escherichia coli
Zea mays
Hydrolysis

Keywords

  • AFEX-treated corn stover
  • Biofuels
  • Endo-xylanase
  • Thermostable

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal
  • Medicine(all)

Cite this

Bhalla, A., Bischoff, K. M., Uppugundla, N., Balan, V., & Sani, R. K. (2014). Novel thermostable endo-xylanase cloned and expressed from bacterium Geobacillus sp. WSUCF1. Bioresource Technology, 165(C), 314-318. DOI: 10.1016/j.biortech.2014.03.112

Novel thermostable endo-xylanase cloned and expressed from bacterium Geobacillus sp. WSUCF1. / Bhalla, Aditya; Bischoff, Kenneth M.; Uppugundla, Nirmal; Balan, Venkatesh; Sani, Rajesh K.

In: Bioresource Technology, Vol. 165, No. C, 2014, p. 314-318.

Research output: Contribution to journalArticle

Bhalla, A, Bischoff, KM, Uppugundla, N, Balan, V & Sani, RK 2014, 'Novel thermostable endo-xylanase cloned and expressed from bacterium Geobacillus sp. WSUCF1' Bioresource Technology, vol. 165, no. C, pp. 314-318. DOI: 10.1016/j.biortech.2014.03.112
Bhalla A, Bischoff KM, Uppugundla N, Balan V, Sani RK. Novel thermostable endo-xylanase cloned and expressed from bacterium Geobacillus sp. WSUCF1. Bioresource Technology. 2014;165(C):314-318. Available from, DOI: 10.1016/j.biortech.2014.03.112
Bhalla, Aditya ; Bischoff, Kenneth M. ; Uppugundla, Nirmal ; Balan, Venkatesh ; Sani, Rajesh K./ Novel thermostable endo-xylanase cloned and expressed from bacterium Geobacillus sp. WSUCF1. In: Bioresource Technology. 2014 ; Vol. 165, No. C. pp. 314-318
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