Novel two-stage fermentation process for bioethanol production using Saccharomyces pastorianus

Yogender Kumar Gowtham, Kristen P. Miller, David B. Hodge, J. Michael Henson, Sarah W. Harcum

Research output: Contribution to journalArticle

  • 3 Citations

Abstract

Bioethanol produced from lignocellulosic materials has the potential to be economically feasible, if both glucose and xylose released from cellulose and hemicellulose can be efficiently converted to ethanol. Saccharomyces spp. can efficiently convert glucose to ethanol; however, xylose conversion to ethanol is a major hurdle due to lack of xylose-metabolizing pathways. In this study, a novel two-stage fermentation process was investigated to improve bioethanol productivity. In this process, xylose is converted into biomass via non-Saccharomyces microorganism and coupled to a glucose-utilizing Saccharomyces fermentation. Escherichia coli was determined to efficiently convert xylose to biomass, which was then killed to produce E. coli extract. Since earlier studies with Saccharomyces pastorianus demonstrated that xylose isomerase increased ethanol productivities on pure sugars, the addition of both E. coli extract and xylose isomerase to S. pastorianus fermentations on pure sugars and corn stover hydrolysates were investigated. It was determined that the xylose isomerase addition increased ethanol productivities on pure sugars but was not as effective alone on the corn stover hydrolysates. It was observed that the E. coli extract addition increased ethanol productivities on both corn stover hydrolysates and pure sugars. The ethanol productivities observed on the corn stover hydrolysates with the E. coli extract addition was the same as observed on pure sugars with both E. coli extract and xylose isomerase additions. These results indicate that the two-stage fermentation process has the capability to be a competitive alternative to recombinant Saccharomyces cerevisiae-based fermentations.

LanguageEnglish (US)
Pages300-310
Number of pages11
JournalBiotechnology Progress
Volume30
Issue number2
DOIs
StatePublished - 2014

Profile

Saccharomyces
xylose isomerase
Fermentation
Ethanol
Xylose
Escherichia coli
Zea mays
Biomass
Glucose
Cellulose
Saccharomyces cerevisiae

Keywords

  • Escherichia coli
  • Hydrolysates
  • Sustainable energy
  • Xylose

ASJC Scopus subject areas

  • Biotechnology
  • Medicine(all)

Cite this

Gowtham, Y. K., Miller, K. P., Hodge, D. B., Henson, J. M., & Harcum, S. W. (2014). Novel two-stage fermentation process for bioethanol production using Saccharomyces pastorianus. Biotechnology Progress, 30(2), 300-310. DOI: 10.1002/btpr.1850

Novel two-stage fermentation process for bioethanol production using Saccharomyces pastorianus. / Gowtham, Yogender Kumar; Miller, Kristen P.; Hodge, David B.; Henson, J. Michael; Harcum, Sarah W.

In: Biotechnology Progress, Vol. 30, No. 2, 2014, p. 300-310.

Research output: Contribution to journalArticle

Gowtham, YK, Miller, KP, Hodge, DB, Henson, JM & Harcum, SW 2014, 'Novel two-stage fermentation process for bioethanol production using Saccharomyces pastorianus' Biotechnology Progress, vol 30, no. 2, pp. 300-310. DOI: 10.1002/btpr.1850
Gowtham YK, Miller KP, Hodge DB, Henson JM, Harcum SW. Novel two-stage fermentation process for bioethanol production using Saccharomyces pastorianus. Biotechnology Progress. 2014;30(2):300-310. Available from, DOI: 10.1002/btpr.1850
Gowtham, Yogender Kumar ; Miller, Kristen P. ; Hodge, David B. ; Henson, J. Michael ; Harcum, Sarah W./ Novel two-stage fermentation process for bioethanol production using Saccharomyces pastorianus. In: Biotechnology Progress. 2014 ; Vol. 30, No. 2. pp. 300-310
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