Novel two-stage fermentation process for bioethanol production using Saccharomyces pastorianus

Yogender Kumar Gowtham, Kristen P. Miller, David B. Hodge, J. Michael Henson, Sarah W. Harcum

    Research output: Research - peer-reviewArticle

    • 3 Citations

    Abstract

    Bioethanol produced from lignocellulosic materials has the potential to be economically feasible, if both glucose and xylose released from cellulose and hemicellulose can be efficiently converted to ethanol. Saccharomyces spp. can efficiently convert glucose to ethanol; however, xylose conversion to ethanol is a major hurdle due to lack of xylose-metabolizing pathways. In this study, a novel two-stage fermentation process was investigated to improve bioethanol productivity. In this process, xylose is converted into biomass via non-Saccharomyces microorganism and coupled to a glucose-utilizing Saccharomyces fermentation. Escherichia coli was determined to efficiently convert xylose to biomass, which was then killed to produce E. coli extract. Since earlier studies with Saccharomyces pastorianus demonstrated that xylose isomerase increased ethanol productivities on pure sugars, the addition of both E. coli extract and xylose isomerase to S. pastorianus fermentations on pure sugars and corn stover hydrolysates were investigated. It was determined that the xylose isomerase addition increased ethanol productivities on pure sugars but was not as effective alone on the corn stover hydrolysates. It was observed that the E. coli extract addition increased ethanol productivities on both corn stover hydrolysates and pure sugars. The ethanol productivities observed on the corn stover hydrolysates with the E. coli extract addition was the same as observed on pure sugars with both E. coli extract and xylose isomerase additions. These results indicate that the two-stage fermentation process has the capability to be a competitive alternative to recombinant Saccharomyces cerevisiae-based fermentations.

    LanguageEnglish (US)
    Pages300-310
    Number of pages11
    JournalBiotechnology Progress
    Volume30
    Issue number2
    DOIs
    StatePublished - 2014

    Profile

    Saccharomyces
    Fermentation
    Ethanol
    Escherichia coli
    xylose isomerase
    Xylose
    Zea mays
    Biomass
    Glucose
    Cellulose
    Saccharomyces cerevisiae
    hemicellulose
    xylose-glucose

    Keywords

    • Escherichia coli
    • Hydrolysates
    • Sustainable energy
    • Xylose

    ASJC Scopus subject areas

    • Biotechnology
    • Medicine(all)

    Cite this

    Novel two-stage fermentation process for bioethanol production using Saccharomyces pastorianus. / Gowtham, Yogender Kumar; Miller, Kristen P.; Hodge, David B.; Henson, J. Michael; Harcum, Sarah W.

    In: Biotechnology Progress, Vol. 30, No. 2, 2014, p. 300-310.

    Research output: Research - peer-reviewArticle

    Gowtham, Yogender Kumar ; Miller, Kristen P. ; Hodge, David B. ; Henson, J. Michael ; Harcum, Sarah W./ Novel two-stage fermentation process for bioethanol production using Saccharomyces pastorianus. In: Biotechnology Progress. 2014 ; Vol. 30, No. 2. pp. 300-310
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