Patterned co-culture of primary hepatocytes and fibroblasts using polyelectrolyte multilayer templates

Srivatsan Kidambi, Lufang Sheng, Martin L. Yarmush, Mehmet Toner, Ilsoon Lee, Christina Chan

    Research output: Research - peer-reviewArticle

    • 53 Citations

    Abstract

    This paper describes the formation of patterned cell co-cultures using the layer-by-layer deposition of synthetic ionic polymers and without the aid of adhesive proteins/ligands such as collagen or fibronectin. In this study, we used synthetic polymers, namely poly(diallyldimethylammonium chloride) (PDAC) and sulfonated polystyrene (SPS) as the polycation and polyanion, respectively, to build the multilayer films. We formed SPS patterns on polyelectrolyte multilayer (PEM) surfaces either by microcontact printing PDAC onto SPS surfaces or vice-versa. To create patterned co-cultures on PEMs, we capitalize on the preferential attachment and spreading of primary hepatocytes on SPS as opposed to PDAC surfaces. In contrast, fibroblasts readily attached to both PDAC and SPS surfaces, and as a result, we were able to obtain patterned co-cultures of fibroblast and primary hepatocytes on synthetic PEM surfaces. We characterized the morphology and hepatic-specific functions of the patterned cell co-cultures with microscopy and biochemical assays. Our results suggest an alternative approach to fabricating controlled co-cultures with specified cell-cell and cell-surface interactions; this approach provides flexibility in designing cell-specific surfaces for tissue engineering applications. (Figure Presented).

    LanguageEnglish (US)
    Pages344-353
    Number of pages10
    JournalMacromolecular Bioscience
    Volume7
    Issue number3
    DOIs
    StatePublished - Mar 8 2007

    Profile

    Polystyrenes
    Coculture Techniques
    Hepatocytes
    Fibroblasts
    Polyelectrolytes
    Cell culture
    Multilayers
    poly-N,N-dimethyl-N,N-diallylammonium chloride
    Polymers
    Cell Culture Techniques
    Printing
    Tissue Engineering
    Fibronectins
    Cell Communication
    Adhesives
    Microscopy
    Collagen
    Ligands
    Liver
    Proteins

    Keywords

    • Adhesion
    • Co-culture
    • Micro-contact printing
    • Polyelectrolyte
    • Polyelectrolyte multilayers
    • Primary hepatocytes
    • Tissue engineering

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Biochemistry
    • Biomaterials
    • Polymers and Plastics
    • Materials Chemistry
    • Bioengineering

    Cite this

    Patterned co-culture of primary hepatocytes and fibroblasts using polyelectrolyte multilayer templates. / Kidambi, Srivatsan; Sheng, Lufang; Yarmush, Martin L.; Toner, Mehmet; Lee, Ilsoon; Chan, Christina.

    In: Macromolecular Bioscience, Vol. 7, No. 3, 08.03.2007, p. 344-353.

    Research output: Research - peer-reviewArticle

    Kidambi, Srivatsan ; Sheng, Lufang ; Yarmush, Martin L. ; Toner, Mehmet ; Lee, Ilsoon ; Chan, Christina. / Patterned co-culture of primary hepatocytes and fibroblasts using polyelectrolyte multilayer templates. In: Macromolecular Bioscience. 2007 ; Vol. 7, No. 3. pp. 344-353
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