Patterns of aptamers on polyelectrolyte multilayers

Srivatsan Kidambi, Ilsoon Lee, Christina Chan

Research output: ResearchConference contribution

Abstract

The ionic layer-by-layer (LBL) assembly technique, introduced by Decher in 1991, is a versatile and inexpensive method of constructing polymeric thin films called "Polyelectrolyte Multilayers (PEMs)", with nanometer-scale control of ionized species. PEMs are excellent candidates for tissue engineering applications due to their biocompatibility and bioinertness, and the ability to incorporate biological molecules, such as proteins. This work describes the successful immobilization and patterning of aptamers on PEM films using electrostatic interaction and covalent crosslinking of nucleic acids on top of thin films. Patterns of thrombin aptamers were formed on PEM surfaces by microcontact printing the nucleic acids onto the PEM surfaces. This technique may be a useful tool for fabricating aptamer arrays to detect and quantitate proteins. Presence and stability of the aptamers on the PEM films were investigated via ellipsometry, FTIR, optical microscope and QCM. Future work will focus on assembling the aptamers to create targeted delivery systems.

LanguageEnglish (US)
Title of host publicationAIChE Annual Meeting, Conference Proceedings
Pages4296
Number of pages1
StatePublished - 2005
Externally publishedYes
Event05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, United States
Duration: Oct 30 2005Nov 4 2005

Other

Other05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
CountryUnited States
CityCincinnati, OH
Period10/30/0511/4/05

Profile

Polyelectrolytes
Multilayers
Multilayer films
Nucleic acids
Proteins
Thin films
Ellipsometry
Coulomb interactions
Biocompatibility
Tissue engineering
Polymer films
Crosslinking
Printing
Microscopes
Molecules

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kidambi, S., Lee, I., & Chan, C. (2005). Patterns of aptamers on polyelectrolyte multilayers. In AIChE Annual Meeting, Conference Proceedings (pp. 4296)

Patterns of aptamers on polyelectrolyte multilayers. / Kidambi, Srivatsan; Lee, Ilsoon; Chan, Christina.

AIChE Annual Meeting, Conference Proceedings. 2005. p. 4296.

Research output: ResearchConference contribution

Kidambi, S, Lee, I & Chan, C 2005, Patterns of aptamers on polyelectrolyte multilayers. in AIChE Annual Meeting, Conference Proceedings. pp. 4296, 05AIChE: 2005 AIChE Annual Meeting and Fall Showcase, Cincinnati, OH, United States, 10/30/05.
Kidambi S, Lee I, Chan C. Patterns of aptamers on polyelectrolyte multilayers. In AIChE Annual Meeting, Conference Proceedings. 2005. p. 4296.
Kidambi, Srivatsan ; Lee, Ilsoon ; Chan, Christina. / Patterns of aptamers on polyelectrolyte multilayers. AIChE Annual Meeting, Conference Proceedings. 2005. pp. 4296
@inbook{41d3a3f3f8164e88b0846580222eb146,
title = "Patterns of aptamers on polyelectrolyte multilayers",
abstract = "The ionic layer-by-layer (LBL) assembly technique, introduced by Decher in 1991, is a versatile and inexpensive method of constructing polymeric thin films called {"}Polyelectrolyte Multilayers (PEMs){"}, with nanometer-scale control of ionized species. PEMs are excellent candidates for tissue engineering applications due to their biocompatibility and bioinertness, and the ability to incorporate biological molecules, such as proteins. This work describes the successful immobilization and patterning of aptamers on PEM films using electrostatic interaction and covalent crosslinking of nucleic acids on top of thin films. Patterns of thrombin aptamers were formed on PEM surfaces by microcontact printing the nucleic acids onto the PEM surfaces. This technique may be a useful tool for fabricating aptamer arrays to detect and quantitate proteins. Presence and stability of the aptamers on the PEM films were investigated via ellipsometry, FTIR, optical microscope and QCM. Future work will focus on assembling the aptamers to create targeted delivery systems.",
author = "Srivatsan Kidambi and Ilsoon Lee and Christina Chan",
year = "2005",
pages = "4296",
booktitle = "AIChE Annual Meeting, Conference Proceedings",

}

TY - CHAP

T1 - Patterns of aptamers on polyelectrolyte multilayers

AU - Kidambi,Srivatsan

AU - Lee,Ilsoon

AU - Chan,Christina

PY - 2005

Y1 - 2005

N2 - The ionic layer-by-layer (LBL) assembly technique, introduced by Decher in 1991, is a versatile and inexpensive method of constructing polymeric thin films called "Polyelectrolyte Multilayers (PEMs)", with nanometer-scale control of ionized species. PEMs are excellent candidates for tissue engineering applications due to their biocompatibility and bioinertness, and the ability to incorporate biological molecules, such as proteins. This work describes the successful immobilization and patterning of aptamers on PEM films using electrostatic interaction and covalent crosslinking of nucleic acids on top of thin films. Patterns of thrombin aptamers were formed on PEM surfaces by microcontact printing the nucleic acids onto the PEM surfaces. This technique may be a useful tool for fabricating aptamer arrays to detect and quantitate proteins. Presence and stability of the aptamers on the PEM films were investigated via ellipsometry, FTIR, optical microscope and QCM. Future work will focus on assembling the aptamers to create targeted delivery systems.

AB - The ionic layer-by-layer (LBL) assembly technique, introduced by Decher in 1991, is a versatile and inexpensive method of constructing polymeric thin films called "Polyelectrolyte Multilayers (PEMs)", with nanometer-scale control of ionized species. PEMs are excellent candidates for tissue engineering applications due to their biocompatibility and bioinertness, and the ability to incorporate biological molecules, such as proteins. This work describes the successful immobilization and patterning of aptamers on PEM films using electrostatic interaction and covalent crosslinking of nucleic acids on top of thin films. Patterns of thrombin aptamers were formed on PEM surfaces by microcontact printing the nucleic acids onto the PEM surfaces. This technique may be a useful tool for fabricating aptamer arrays to detect and quantitate proteins. Presence and stability of the aptamers on the PEM films were investigated via ellipsometry, FTIR, optical microscope and QCM. Future work will focus on assembling the aptamers to create targeted delivery systems.

UR - http://www.scopus.com/inward/record.url?scp=33645651176&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645651176&partnerID=8YFLogxK

M3 - Conference contribution

SP - 4296

BT - AIChE Annual Meeting, Conference Proceedings

ER -