Plasmid-based one-pot saturation mutagenesis

Emily E. Wrenbeck, Justin R. Klesmith, James A. Stapleton, Adebola Adeniran, Keith E.J. Tyo, Timothy A. Whitehead

    Research output: Contribution to journalArticle

    • 4 Citations

    Abstract

    Deep mutational scanning is a foundational tool for addressing the functional consequences of large numbers of mutants, but a more efficient and accessible method for construction of user-defined mutagenesis libraries is needed. Here we present nicking mutagenesis, a robust, single-day, one-pot saturation mutagenesis method performed on routinely prepped plasmid dsDNA. The method can be used to produce comprehensive or single- or multi-site saturation mutagenesis libraries.

    Original languageEnglish (US)
    Pages (from-to)928-930
    Number of pages3
    JournalNature Methods
    Volume13
    Issue number11
    DOIs
    StatePublished - Nov 1 2016

    Profile

    Mutagenesis
    Plasmids
    Insulin Infusion Systems
    Breast Cyst
    Scanning

    ASJC Scopus subject areas

    • Biotechnology
    • Molecular Biology
    • Biochemistry
    • Cell Biology

    Cite this

    Wrenbeck, E. E., Klesmith, J. R., Stapleton, J. A., Adeniran, A., Tyo, K. E. J., & Whitehead, T. A. (2016). Plasmid-based one-pot saturation mutagenesis. Nature Methods, 13(11), 928-930. DOI: 10.1038/nmeth.4029

    Plasmid-based one-pot saturation mutagenesis. / Wrenbeck, Emily E.; Klesmith, Justin R.; Stapleton, James A.; Adeniran, Adebola; Tyo, Keith E.J.; Whitehead, Timothy A.

    In: Nature Methods, Vol. 13, No. 11, 01.11.2016, p. 928-930.

    Research output: Contribution to journalArticle

    Wrenbeck, EE, Klesmith, JR, Stapleton, JA, Adeniran, A, Tyo, KEJ & Whitehead, TA 2016, 'Plasmid-based one-pot saturation mutagenesis' Nature Methods, vol 13, no. 11, pp. 928-930. DOI: 10.1038/nmeth.4029
    Wrenbeck EE, Klesmith JR, Stapleton JA, Adeniran A, Tyo KEJ, Whitehead TA. Plasmid-based one-pot saturation mutagenesis. Nature Methods. 2016 Nov 1;13(11):928-930. Available from, DOI: 10.1038/nmeth.4029

    Wrenbeck, Emily E.; Klesmith, Justin R.; Stapleton, James A.; Adeniran, Adebola; Tyo, Keith E.J.; Whitehead, Timothy A. / Plasmid-based one-pot saturation mutagenesis.

    In: Nature Methods, Vol. 13, No. 11, 01.11.2016, p. 928-930.

    Research output: Contribution to journalArticle

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