Poly(lactic-co-glycolic acid) encapsulated gadolinium oxide nanoparticles for MRI-based cell tracking

Margaret F. Bennewitz, Simone S. Williams, Michael K. Nkansah, Erik M. Shapiro

Research output: Contribution to journalArticle

  • 9 Citations

Abstract

Superparamagnetic iron oxide particles have proven useful for cell tracking applications by monitoring cell transplantation and migration in living organisms. However, one perceived drawback is that these particles cause dark contrast in MRI, sometimes yielding confusion with other biological phenomena, which also yield dark contrast. To that end, researchers have investigated the use of gadolinium oxide (Gd2O3) based contrast agents for MRI-based cell tracking, as Gd2O3 has favorable r 1 molar relaxivity. We synthesized Gd2O3 nanocrystals and encapsulated them within PLGA matrices to form ∼150 nm nanoparticles. r1 was 1.9 mM-1 sec-1 and r 2 was 8.4 mM-1 sec-1. Cell labeling with particles was well tolerated by cells except at very high doses. MRI of labeled cells showed that labeled cells could achieve both R1 and R 2 enhancements due to the internalized particles. R2 enhancements were ∼ twice that of R1 enhancements suggesting the use of very short echo times when using Gd2O3 based contrast agents for MRI-based cell tracking.

Original languageEnglish (US)
Pages (from-to)3778-3783
Number of pages6
JournalJournal of Nanoscience and Nanotechnology
Volume13
Issue number6
DOIs
StatePublished - Jun 2013
Externally publishedYes

Profile

cells
Magnetic resonance imaging
Gadolinium
Nanoparticles
Oxides
Myosins
augmentation
gadolinium
nanoparticles
oxides
Iron oxides
Nanocrystals
Labeling
Acids
Monitoring
Enzyme Reactivators
Nicarbazin
Antimony Potassium Tartrate
Mastication
transplantation

Keywords

  • Cells
  • Gadolinium oxide
  • MRI
  • Particles
  • PLGA

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Chemistry(all)
  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering

Cite this

Poly(lactic-co-glycolic acid) encapsulated gadolinium oxide nanoparticles for MRI-based cell tracking. / Bennewitz, Margaret F.; Williams, Simone S.; Nkansah, Michael K.; Shapiro, Erik M.

In: Journal of Nanoscience and Nanotechnology, Vol. 13, No. 6, 06.2013, p. 3778-3783.

Research output: Contribution to journalArticle

Bennewitz, Margaret F.; Williams, Simone S.; Nkansah, Michael K.; Shapiro, Erik M. / Poly(lactic-co-glycolic acid) encapsulated gadolinium oxide nanoparticles for MRI-based cell tracking.

In: Journal of Nanoscience and Nanotechnology, Vol. 13, No. 6, 06.2013, p. 3778-3783.

Research output: Contribution to journalArticle

@article{e159ae0eebfa4fe58fc7ead60e7cfc21,
title = "Poly(lactic-co-glycolic acid) encapsulated gadolinium oxide nanoparticles for MRI-based cell tracking",
abstract = "Superparamagnetic iron oxide particles have proven useful for cell tracking applications by monitoring cell transplantation and migration in living organisms. However, one perceived drawback is that these particles cause dark contrast in MRI, sometimes yielding confusion with other biological phenomena, which also yield dark contrast. To that end, researchers have investigated the use of gadolinium oxide (Gd2O3) based contrast agents for MRI-based cell tracking, as Gd2O3 has favorable r 1 molar relaxivity. We synthesized Gd2O3 nanocrystals and encapsulated them within PLGA matrices to form ∼150 nm nanoparticles. r1 was 1.9 mM-1 sec-1 and r 2 was 8.4 mM-1 sec-1. Cell labeling with particles was well tolerated by cells except at very high doses. MRI of labeled cells showed that labeled cells could achieve both R1 and R 2 enhancements due to the internalized particles. R2 enhancements were ∼ twice that of R1 enhancements suggesting the use of very short echo times when using Gd2O3 based contrast agents for MRI-based cell tracking.",
keywords = "Cells, Gadolinium oxide, MRI, Particles, PLGA",
author = "Bennewitz, {Margaret F.} and Williams, {Simone S.} and Nkansah, {Michael K.} and Shapiro, {Erik M.}",
year = "2013",
month = "6",
doi = "10.1166/jnn.2013.7435",
volume = "13",
pages = "3778--3783",
journal = "Journal of Nanoscience and Nanotechnology",
issn = "1533-4880",
publisher = "American Scientific Publishers",
number = "6",

}

TY - JOUR

T1 - Poly(lactic-co-glycolic acid) encapsulated gadolinium oxide nanoparticles for MRI-based cell tracking

AU - Bennewitz,Margaret F.

AU - Williams,Simone S.

AU - Nkansah,Michael K.

AU - Shapiro,Erik M.

PY - 2013/6

Y1 - 2013/6

N2 - Superparamagnetic iron oxide particles have proven useful for cell tracking applications by monitoring cell transplantation and migration in living organisms. However, one perceived drawback is that these particles cause dark contrast in MRI, sometimes yielding confusion with other biological phenomena, which also yield dark contrast. To that end, researchers have investigated the use of gadolinium oxide (Gd2O3) based contrast agents for MRI-based cell tracking, as Gd2O3 has favorable r 1 molar relaxivity. We synthesized Gd2O3 nanocrystals and encapsulated them within PLGA matrices to form ∼150 nm nanoparticles. r1 was 1.9 mM-1 sec-1 and r 2 was 8.4 mM-1 sec-1. Cell labeling with particles was well tolerated by cells except at very high doses. MRI of labeled cells showed that labeled cells could achieve both R1 and R 2 enhancements due to the internalized particles. R2 enhancements were ∼ twice that of R1 enhancements suggesting the use of very short echo times when using Gd2O3 based contrast agents for MRI-based cell tracking.

AB - Superparamagnetic iron oxide particles have proven useful for cell tracking applications by monitoring cell transplantation and migration in living organisms. However, one perceived drawback is that these particles cause dark contrast in MRI, sometimes yielding confusion with other biological phenomena, which also yield dark contrast. To that end, researchers have investigated the use of gadolinium oxide (Gd2O3) based contrast agents for MRI-based cell tracking, as Gd2O3 has favorable r 1 molar relaxivity. We synthesized Gd2O3 nanocrystals and encapsulated them within PLGA matrices to form ∼150 nm nanoparticles. r1 was 1.9 mM-1 sec-1 and r 2 was 8.4 mM-1 sec-1. Cell labeling with particles was well tolerated by cells except at very high doses. MRI of labeled cells showed that labeled cells could achieve both R1 and R 2 enhancements due to the internalized particles. R2 enhancements were ∼ twice that of R1 enhancements suggesting the use of very short echo times when using Gd2O3 based contrast agents for MRI-based cell tracking.

KW - Cells

KW - Gadolinium oxide

KW - MRI

KW - Particles

KW - PLGA

UR - http://www.scopus.com/inward/record.url?scp=84878744399&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878744399&partnerID=8YFLogxK

U2 - 10.1166/jnn.2013.7435

DO - 10.1166/jnn.2013.7435

M3 - Article

VL - 13

SP - 3778

EP - 3783

JO - Journal of Nanoscience and Nanotechnology

T2 - Journal of Nanoscience and Nanotechnology

JF - Journal of Nanoscience and Nanotechnology

SN - 1533-4880

IS - 6

ER -