Probing the nature of AFEX-pretreated corn stover derived decomposition products that inhibit cellulase activity

James F. Humpula, Nirmal Uppugundla, Ramin Vismeh, Leonardo Sousa, Shishir P S Chundawat, A. Daniel Jones, Venkatesh Balan, Bruce E. Dale, Albert M. Cheh

Research output: Contribution to journalArticle

  • 6 Citations

Abstract

Sequential fractionation of AFEX-pretreated corn stover extracts was carried out using ultra-centrifugation, ultra-filtration, and solid phase extraction to isolate various classes of pretreatment products to evaluate their inhibitory effect on cellulases. Ultra-centrifugation removed dark brown precipitates that caused no appreciable enzyme inhibition. Ultra-filtration of ultra-centrifuged AFEX-pretreated corn stover extractives using a 10. kDa molecular weight cutoff (MWCO) membrane removed additional high molecular weight components that accounted for 24-28% of the total observed enzyme inhibition while a 3. kDa MWCO membrane removed 60-65%, suggesting significant inhibition is caused by oligomeric materials. Solid phase extraction (SPE) of AFEX-pretreated corn stover extractives after ultra-centrifugation removed 34-43% of the inhibition; ultra-filtration with a 5. kDa membrane removed 44-56% of the inhibition and when this ultra-filtrate was subjected to SPE a total of 69-70% of the inhibition were removed. Mass spectrometry found several phenolic compounds among the hydrophobic inhibition removed by SPE adsorption.

LanguageEnglish (US)
Pages38-45
Number of pages8
JournalBioresource Technology
Volume152
DOIs
StatePublished - 2014

Profile

Cellulase
Ultrafiltration
maize
decomposition
Decomposition
Enzyme inhibition
Molecular weight
Membranes
ultrafiltration
Cellulases
membrane
Fractionation
Mass spectrometry
Precipitates
enzyme
Adsorption
phenolic compound
product
fractionation
mass spectrometry

Keywords

  • AFEX
  • Cellulase
  • Fractionation
  • Inhibition
  • Ultra-filtration

ASJC Scopus subject areas

  • Waste Management and Disposal
  • Bioengineering
  • Environmental Engineering

Cite this

Probing the nature of AFEX-pretreated corn stover derived decomposition products that inhibit cellulase activity. / Humpula, James F.; Uppugundla, Nirmal; Vismeh, Ramin; Sousa, Leonardo; Chundawat, Shishir P S; Jones, A. Daniel; Balan, Venkatesh; Dale, Bruce E.; Cheh, Albert M.

In: Bioresource Technology, Vol. 152, 2014, p. 38-45.

Research output: Contribution to journalArticle

Humpula, James F. ; Uppugundla, Nirmal ; Vismeh, Ramin ; Sousa, Leonardo ; Chundawat, Shishir P S ; Jones, A. Daniel ; Balan, Venkatesh ; Dale, Bruce E. ; Cheh, Albert M./ Probing the nature of AFEX-pretreated corn stover derived decomposition products that inhibit cellulase activity. In: Bioresource Technology. 2014 ; Vol. 152. pp. 38-45
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