Probing the nature of AFEX-pretreated corn stover derived decomposition products that inhibit cellulase activity

James F. Humpula, Nirmal Uppugundla, Ramin Vismeh, Leonardo Sousa, Shishir P S Chundawat, A. Daniel Jones, Venkatesh Balan, Bruce E. Dale, Albert M. Cheh

    Research output: Research - peer-reviewArticle

    • 5 Citations

    Abstract

    Sequential fractionation of AFEX-pretreated corn stover extracts was carried out using ultra-centrifugation, ultra-filtration, and solid phase extraction to isolate various classes of pretreatment products to evaluate their inhibitory effect on cellulases. Ultra-centrifugation removed dark brown precipitates that caused no appreciable enzyme inhibition. Ultra-filtration of ultra-centrifuged AFEX-pretreated corn stover extractives using a 10. kDa molecular weight cutoff (MWCO) membrane removed additional high molecular weight components that accounted for 24-28% of the total observed enzyme inhibition while a 3. kDa MWCO membrane removed 60-65%, suggesting significant inhibition is caused by oligomeric materials. Solid phase extraction (SPE) of AFEX-pretreated corn stover extractives after ultra-centrifugation removed 34-43% of the inhibition; ultra-filtration with a 5. kDa membrane removed 44-56% of the inhibition and when this ultra-filtrate was subjected to SPE a total of 69-70% of the inhibition were removed. Mass spectrometry found several phenolic compounds among the hydrophobic inhibition removed by SPE adsorption.

    LanguageEnglish (US)
    Pages38-45
    Number of pages8
    JournalBioresource Technology
    Volume152
    DOIs
    StatePublished - 2014

    Profile

    Cellulase
    Decomposition
    maize
    decomposition
    product
    Ultrafiltration
    Molecular weight
    Membranes
    Ultracentrifugation
    Enzyme inhibition
    ultrafiltration
    membrane
    centrifugation
    enzyme
    Cellulases
    Fractionation
    Mass spectrometry
    Precipitates
    Adsorption
    phenolic compound

    Keywords

    • AFEX
    • Cellulase
    • Fractionation
    • Inhibition
    • Ultra-filtration

    ASJC Scopus subject areas

    • Waste Management and Disposal
    • Bioengineering
    • Environmental Engineering

    Cite this

    Humpula, J. F., Uppugundla, N., Vismeh, R., Sousa, L., Chundawat, S. P. S., Jones, A. D., ... Cheh, A. M. (2014). Probing the nature of AFEX-pretreated corn stover derived decomposition products that inhibit cellulase activity. Bioresource Technology, 152, 38-45. DOI: 10.1016/j.biortech.2013.10.082

    Probing the nature of AFEX-pretreated corn stover derived decomposition products that inhibit cellulase activity. / Humpula, James F.; Uppugundla, Nirmal; Vismeh, Ramin; Sousa, Leonardo; Chundawat, Shishir P S; Jones, A. Daniel; Balan, Venkatesh; Dale, Bruce E.; Cheh, Albert M.

    In: Bioresource Technology, Vol. 152, 2014, p. 38-45.

    Research output: Research - peer-reviewArticle

    Humpula, James F. ; Uppugundla, Nirmal ; Vismeh, Ramin ; Sousa, Leonardo ; Chundawat, Shishir P S ; Jones, A. Daniel ; Balan, Venkatesh ; Dale, Bruce E. ; Cheh, Albert M./ Probing the nature of AFEX-pretreated corn stover derived decomposition products that inhibit cellulase activity. In: Bioresource Technology. 2014 ; Vol. 152. pp. 38-45
    @article{64635b13d0104ccfabad079ecaa36cc0,
    title = "Probing the nature of AFEX-pretreated corn stover derived decomposition products that inhibit cellulase activity",
    abstract = "Sequential fractionation of AFEX-pretreated corn stover extracts was carried out using ultra-centrifugation, ultra-filtration, and solid phase extraction to isolate various classes of pretreatment products to evaluate their inhibitory effect on cellulases. Ultra-centrifugation removed dark brown precipitates that caused no appreciable enzyme inhibition. Ultra-filtration of ultra-centrifuged AFEX-pretreated corn stover extractives using a 10. kDa molecular weight cutoff (MWCO) membrane removed additional high molecular weight components that accounted for 24-28% of the total observed enzyme inhibition while a 3. kDa MWCO membrane removed 60-65%, suggesting significant inhibition is caused by oligomeric materials. Solid phase extraction (SPE) of AFEX-pretreated corn stover extractives after ultra-centrifugation removed 34-43% of the inhibition; ultra-filtration with a 5. kDa membrane removed 44-56% of the inhibition and when this ultra-filtrate was subjected to SPE a total of 69-70% of the inhibition were removed. Mass spectrometry found several phenolic compounds among the hydrophobic inhibition removed by SPE adsorption.",
    keywords = "AFEX, Cellulase, Fractionation, Inhibition, Ultra-filtration",
    author = "Humpula, {James F.} and Nirmal Uppugundla and Ramin Vismeh and Leonardo Sousa and Chundawat, {Shishir P S} and Jones, {A. Daniel} and Venkatesh Balan and Dale, {Bruce E.} and Cheh, {Albert M.}",
    year = "2014",
    doi = "10.1016/j.biortech.2013.10.082",
    volume = "152",
    pages = "38--45",
    journal = "Bioresource Technology",
    issn = "0960-8524",
    publisher = "Elsevier Limited",

    }

    TY - JOUR

    T1 - Probing the nature of AFEX-pretreated corn stover derived decomposition products that inhibit cellulase activity

    AU - Humpula,James F.

    AU - Uppugundla,Nirmal

    AU - Vismeh,Ramin

    AU - Sousa,Leonardo

    AU - Chundawat,Shishir P S

    AU - Jones,A. Daniel

    AU - Balan,Venkatesh

    AU - Dale,Bruce E.

    AU - Cheh,Albert M.

    PY - 2014

    Y1 - 2014

    N2 - Sequential fractionation of AFEX-pretreated corn stover extracts was carried out using ultra-centrifugation, ultra-filtration, and solid phase extraction to isolate various classes of pretreatment products to evaluate their inhibitory effect on cellulases. Ultra-centrifugation removed dark brown precipitates that caused no appreciable enzyme inhibition. Ultra-filtration of ultra-centrifuged AFEX-pretreated corn stover extractives using a 10. kDa molecular weight cutoff (MWCO) membrane removed additional high molecular weight components that accounted for 24-28% of the total observed enzyme inhibition while a 3. kDa MWCO membrane removed 60-65%, suggesting significant inhibition is caused by oligomeric materials. Solid phase extraction (SPE) of AFEX-pretreated corn stover extractives after ultra-centrifugation removed 34-43% of the inhibition; ultra-filtration with a 5. kDa membrane removed 44-56% of the inhibition and when this ultra-filtrate was subjected to SPE a total of 69-70% of the inhibition were removed. Mass spectrometry found several phenolic compounds among the hydrophobic inhibition removed by SPE adsorption.

    AB - Sequential fractionation of AFEX-pretreated corn stover extracts was carried out using ultra-centrifugation, ultra-filtration, and solid phase extraction to isolate various classes of pretreatment products to evaluate their inhibitory effect on cellulases. Ultra-centrifugation removed dark brown precipitates that caused no appreciable enzyme inhibition. Ultra-filtration of ultra-centrifuged AFEX-pretreated corn stover extractives using a 10. kDa molecular weight cutoff (MWCO) membrane removed additional high molecular weight components that accounted for 24-28% of the total observed enzyme inhibition while a 3. kDa MWCO membrane removed 60-65%, suggesting significant inhibition is caused by oligomeric materials. Solid phase extraction (SPE) of AFEX-pretreated corn stover extractives after ultra-centrifugation removed 34-43% of the inhibition; ultra-filtration with a 5. kDa membrane removed 44-56% of the inhibition and when this ultra-filtrate was subjected to SPE a total of 69-70% of the inhibition were removed. Mass spectrometry found several phenolic compounds among the hydrophobic inhibition removed by SPE adsorption.

    KW - AFEX

    KW - Cellulase

    KW - Fractionation

    KW - Inhibition

    KW - Ultra-filtration

    UR - http://www.scopus.com/inward/record.url?scp=84888111960&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84888111960&partnerID=8YFLogxK

    U2 - 10.1016/j.biortech.2013.10.082

    DO - 10.1016/j.biortech.2013.10.082

    M3 - Article

    VL - 152

    SP - 38

    EP - 45

    JO - Bioresource Technology

    T2 - Bioresource Technology

    JF - Bioresource Technology

    SN - 0960-8524

    ER -