Production of Seed-Like Storage Lipids and Increase in Oil Bodies in Corn (Maize; Zea mays L.) Vegetative Biomass

Hussien Alameldin, Ali Izadi-Darbandi, Scott A. Smith, Venkatesh Balan, A. Daniel Jones, Mariam Sticklen

Research output: Research - peer-reviewArticle

Abstract

Triacylglycerides (TAGs) are high energy density lipids with a $25 billion commodity at international level. Plant TAGs are produced via a series of chemical reactions catalyzed by certain enzymes including diacylglycerol acyltransferases (DGATs) and phospholipid diacylglycerol acyltransferases (PDATs). In Arabidopsis, certain transcription factors including Wrinkled 1 (Wri1) and Leafy Cotyledons2 (LEC2) supply the necessary substrates for synthesis of fatty acids and/or for packaging of the oil bodies. TAGs will be stored as oil body structurs which certain proteins such as oleosin can protect them from Enzymatic degradation. In this Study, we overexpressed three major genes involved in the TAG biosynthesis and accumulation; 1) dgat1 as a key enzyme in TAGs biosynthesis, 2) wri1 which is the major transcription factor involved in supplies of fatty acids for TAG biosynthesis, and 3) oleosin (Ole) gene which encodes for protein that protects TAGs from degradation. All three genes were integrated in maize genome under a constitutive promoter to allow the production of oil bodies and seed storage oil-like TAG in maize vegetative biomass. Our results indicated an increase in the total leaf fatty acid contents by 71% (from 1.35 to 2.31 mg/g dry weight) in the metabolically engineered line. GC-MS analysis detected a total of 21 fatty acids in the leaf oil extract samples, representing ∼99.99% of the total fatty acids. Overall, the percentage of the leaf total saturated and monounsaturated fatty acids (MUFA) were increased while the percentage of polyunsaturated fatty acids (PUFA) were decreased in metabolically engineered plant leaves. This is the first report of increasing TAG accumulation in maize vegetative biomass (stover) via metabolic engineering, which will open new dimension for creating new opportunities to complement and expedite the cellulosic biofuels applications.

LanguageEnglish (US)
Pages526-534
Number of pages9
JournalIndustrial Crops and Products
Volume108
DOIs
StatePublished - Dec 1 2017

Profile

lipid bodies
seed crop production
Zea mays
fatty acids
corn
biomass
lipids
biosynthesis
leaves
oleosin
diacylglycerol acyltransferase
transcription factors
degradation
enzymes
genes
proteins
metabolic engineering
stover
leaf oils
seed storage

Keywords

  • Biofuel
  • Corn
  • Dgat1
  • Ole
  • TAG
  • Wri1

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Production of Seed-Like Storage Lipids and Increase in Oil Bodies in Corn (Maize; Zea mays L.) Vegetative Biomass. / Alameldin, Hussien; Izadi-Darbandi, Ali; Smith, Scott A.; Balan, Venkatesh; Jones, A. Daniel; Sticklen, Mariam.

In: Industrial Crops and Products, Vol. 108, 01.12.2017, p. 526-534.

Research output: Research - peer-reviewArticle

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