Quantifying pretreatment degradation compounds in solution and accumulated by cells during solids and yeast recycling in the Rapid Bioconversion with Integrated recycling Technology process using AFEX™ corn stover

Cory Sarks, Alan Higbee, Jeff Piotrowski, Saisi Xue, Joshua J. Coon, Trey K. Sato, Mingjie Jin, Venkatesh Balan, Bruce E. Dale

Research output: Contribution to journalArticle

  • 4 Citations

Abstract

Effects of degradation products (low molecular weight compounds produced during pretreatment) on the microbes used in the RaBIT (Rapid Bioconversion with Integrated recycling Technology) process that reduces enzyme usage up to 40% by efficient enzyme recycling were studied. Chemical genomic profiling was performed, showing no yeast response differences in hydrolysates produced during RaBIT enzymatic hydrolysis. Concentrations of degradation products in solution were quantified after different enzymatic hydrolysis cycles and fermentation cycles. Intracellular degradation product concentrations were also measured following fermentation. Degradation product concentrations in hydrolysate did not change between RaBIT enzymatic hydrolysis cycles; the cell population retained its ability to oxidize/reduce (detoxify) aldehydes over five RaBIT fermentation cycles; and degradation products accumulated within or on the cells as RaBIT fermentation cycles increased. Synthetic hydrolysate was used to confirm that pretreatment degradation products are the sole cause of decreased xylose consumption during RaBIT fermentations.

LanguageEnglish (US)
Pages24-33
Number of pages10
JournalBioresource Technology
Volume205
DOIs
StatePublished - Apr 1 2016

Profile

Bioconversion
Yeast
yeast
Recycling
recycling
maize
Fermentation
fermentation
Degradation
degradation
Enzymatic hydrolysis
hydrolysis
Enzymes
enzyme
Xylose
aldehyde
process technology
Aldehydes
degradation product
genomics

Keywords

  • AFEX™
  • Cell recycling
  • Cellulosic ethanol
  • Pretreatment degradation products
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Quantifying pretreatment degradation compounds in solution and accumulated by cells during solids and yeast recycling in the Rapid Bioconversion with Integrated recycling Technology process using AFEX™ corn stover. / Sarks, Cory; Higbee, Alan; Piotrowski, Jeff; Xue, Saisi; Coon, Joshua J.; Sato, Trey K.; Jin, Mingjie; Balan, Venkatesh; Dale, Bruce E.

In: Bioresource Technology, Vol. 205, 01.04.2016, p. 24-33.

Research output: Contribution to journalArticle

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