Quantifying pretreatment degradation compounds in solution and accumulated by cells during solids and yeast recycling in the Rapid Bioconversion with Integrated recycling Technology process using AFEX™ corn stover

Cory Sarks, Alan Higbee, Jeff Piotrowski, Saisi Xue, Joshua J. Coon, Trey K. Sato, Mingjie Jin, Venkatesh Balan, Bruce E. Dale

    Research output: Contribution to journalArticle

    • 3 Citations

    Abstract

    Effects of degradation products (low molecular weight compounds produced during pretreatment) on the microbes used in the RaBIT (Rapid Bioconversion with Integrated recycling Technology) process that reduces enzyme usage up to 40% by efficient enzyme recycling were studied. Chemical genomic profiling was performed, showing no yeast response differences in hydrolysates produced during RaBIT enzymatic hydrolysis. Concentrations of degradation products in solution were quantified after different enzymatic hydrolysis cycles and fermentation cycles. Intracellular degradation product concentrations were also measured following fermentation. Degradation product concentrations in hydrolysate did not change between RaBIT enzymatic hydrolysis cycles; the cell population retained its ability to oxidize/reduce (detoxify) aldehydes over five RaBIT fermentation cycles; and degradation products accumulated within or on the cells as RaBIT fermentation cycles increased. Synthetic hydrolysate was used to confirm that pretreatment degradation products are the sole cause of decreased xylose consumption during RaBIT fermentations.

    Original languageEnglish (US)
    Pages (from-to)24-33
    Number of pages10
    JournalBioresource Technology
    Volume205
    DOIs
    StatePublished - Apr 1 2016

    Profile

    Recycling
    recycling
    Acyclic Acids
    4-Aminobutyrate Transaminase
    Bioconversion
    Degradation
    degradation
    Fermentation
    fermentation
    Enzymatic hydrolysis
    hydrolysis
    HLA Antigens
    Yeast
    Enzymes
    yeast
    enzyme
    Butylene Glycols
    Castration
    Molecular weight
    Xylose

    Keywords

    • AFEX™
    • Cell recycling
    • Cellulosic ethanol
    • Pretreatment degradation products
    • Saccharomyces cerevisiae

    ASJC Scopus subject areas

    • Bioengineering
    • Environmental Engineering
    • Waste Management and Disposal

    Cite this

    Quantifying pretreatment degradation compounds in solution and accumulated by cells during solids and yeast recycling in the Rapid Bioconversion with Integrated recycling Technology process using AFEX™ corn stover. / Sarks, Cory; Higbee, Alan; Piotrowski, Jeff; Xue, Saisi; Coon, Joshua J.; Sato, Trey K.; Jin, Mingjie; Balan, Venkatesh; Dale, Bruce E.

    In: Bioresource Technology, Vol. 205, 01.04.2016, p. 24-33.

    Research output: Contribution to journalArticle

    Sarks, Cory; Higbee, Alan; Piotrowski, Jeff; Xue, Saisi; Coon, Joshua J.; Sato, Trey K.; Jin, Mingjie; Balan, Venkatesh; Dale, Bruce E. / Quantifying pretreatment degradation compounds in solution and accumulated by cells during solids and yeast recycling in the Rapid Bioconversion with Integrated recycling Technology process using AFEX™ corn stover.

    In: Bioresource Technology, Vol. 205, 01.04.2016, p. 24-33.

    Research output: Contribution to journalArticle

    @article{794ef8224bc24940b11fa947f4a3536e,
    title = "Quantifying pretreatment degradation compounds in solution and accumulated by cells during solids and yeast recycling in the Rapid Bioconversion with Integrated recycling Technology process using AFEX™ corn stover",
    abstract = "Effects of degradation products (low molecular weight compounds produced during pretreatment) on the microbes used in the RaBIT (Rapid Bioconversion with Integrated recycling Technology) process that reduces enzyme usage up to 40% by efficient enzyme recycling were studied. Chemical genomic profiling was performed, showing no yeast response differences in hydrolysates produced during RaBIT enzymatic hydrolysis. Concentrations of degradation products in solution were quantified after different enzymatic hydrolysis cycles and fermentation cycles. Intracellular degradation product concentrations were also measured following fermentation. Degradation product concentrations in hydrolysate did not change between RaBIT enzymatic hydrolysis cycles; the cell population retained its ability to oxidize/reduce (detoxify) aldehydes over five RaBIT fermentation cycles; and degradation products accumulated within or on the cells as RaBIT fermentation cycles increased. Synthetic hydrolysate was used to confirm that pretreatment degradation products are the sole cause of decreased xylose consumption during RaBIT fermentations.",
    keywords = "AFEX™, Cell recycling, Cellulosic ethanol, Pretreatment degradation products, Saccharomyces cerevisiae",
    author = "Cory Sarks and Alan Higbee and Jeff Piotrowski and Saisi Xue and Coon, {Joshua J.} and Sato, {Trey K.} and Mingjie Jin and Venkatesh Balan and Dale, {Bruce E.}",
    year = "2016",
    month = "4",
    doi = "10.1016/j.biortech.2016.01.008",
    volume = "205",
    pages = "24--33",
    journal = "Bioresource Technology",
    issn = "0960-8524",
    publisher = "Elsevier Limited",

    }

    TY - JOUR

    T1 - Quantifying pretreatment degradation compounds in solution and accumulated by cells during solids and yeast recycling in the Rapid Bioconversion with Integrated recycling Technology process using AFEX™ corn stover

    AU - Sarks,Cory

    AU - Higbee,Alan

    AU - Piotrowski,Jeff

    AU - Xue,Saisi

    AU - Coon,Joshua J.

    AU - Sato,Trey K.

    AU - Jin,Mingjie

    AU - Balan,Venkatesh

    AU - Dale,Bruce E.

    PY - 2016/4/1

    Y1 - 2016/4/1

    N2 - Effects of degradation products (low molecular weight compounds produced during pretreatment) on the microbes used in the RaBIT (Rapid Bioconversion with Integrated recycling Technology) process that reduces enzyme usage up to 40% by efficient enzyme recycling were studied. Chemical genomic profiling was performed, showing no yeast response differences in hydrolysates produced during RaBIT enzymatic hydrolysis. Concentrations of degradation products in solution were quantified after different enzymatic hydrolysis cycles and fermentation cycles. Intracellular degradation product concentrations were also measured following fermentation. Degradation product concentrations in hydrolysate did not change between RaBIT enzymatic hydrolysis cycles; the cell population retained its ability to oxidize/reduce (detoxify) aldehydes over five RaBIT fermentation cycles; and degradation products accumulated within or on the cells as RaBIT fermentation cycles increased. Synthetic hydrolysate was used to confirm that pretreatment degradation products are the sole cause of decreased xylose consumption during RaBIT fermentations.

    AB - Effects of degradation products (low molecular weight compounds produced during pretreatment) on the microbes used in the RaBIT (Rapid Bioconversion with Integrated recycling Technology) process that reduces enzyme usage up to 40% by efficient enzyme recycling were studied. Chemical genomic profiling was performed, showing no yeast response differences in hydrolysates produced during RaBIT enzymatic hydrolysis. Concentrations of degradation products in solution were quantified after different enzymatic hydrolysis cycles and fermentation cycles. Intracellular degradation product concentrations were also measured following fermentation. Degradation product concentrations in hydrolysate did not change between RaBIT enzymatic hydrolysis cycles; the cell population retained its ability to oxidize/reduce (detoxify) aldehydes over five RaBIT fermentation cycles; and degradation products accumulated within or on the cells as RaBIT fermentation cycles increased. Synthetic hydrolysate was used to confirm that pretreatment degradation products are the sole cause of decreased xylose consumption during RaBIT fermentations.

    KW - AFEX™

    KW - Cell recycling

    KW - Cellulosic ethanol

    KW - Pretreatment degradation products

    KW - Saccharomyces cerevisiae

    UR - http://www.scopus.com/inward/record.url?scp=84955602711&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=84955602711&partnerID=8YFLogxK

    U2 - 10.1016/j.biortech.2016.01.008

    DO - 10.1016/j.biortech.2016.01.008

    M3 - Article

    VL - 205

    SP - 24

    EP - 33

    JO - Bioresource Technology

    T2 - Bioresource Technology

    JF - Bioresource Technology

    SN - 0960-8524

    ER -