Quantitative analysis of bioactive NAD+ regenerated by NADH electro-oxidation

Hanzi Li, Kathryn E. Worley, Scott Calabrese Barton

    Research output: Contribution to journalArticle

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    Abstract

    The bioactivity of NAD+ electrogenerated at a high-surface-area composite anode was verified spectroscopically. The anode was composed of poly(methylene green) electropolymerized on carbon nanotubes (PMG-CNT) which was in turn immobilized on carbon paper. A mathematical model calibrated by measurements of NADH oxidation at PMG-CNT-modified glassy carbon electrodes was applied to predict transient NADH consumption. The model showed good agreement with the experimental data, and 80% conversion of NADH was observed after 1 h of electrochemical oxidation. Using a spectroscopic enzyme cycling assay, the yield of enzymatically active NAD+ was verified at 93% and 87% for applied potentials of 500 and 150 mV vs Ag|AgCl, respectively. This suggests that roughly 10% of oxidized NADH may be lost due to dimerization or some other side reaction after accounting for self-decay. These results prove that bioactive NAD+ can be efficiently produced using electrochemical techniques, enabling application in bioconversion, biosensor, and bioenergy processes.

    Original languageEnglish (US)
    Pages (from-to)2572-2576
    Number of pages5
    JournalACS Catalysis
    Volume2
    Issue number12
    DOIs
    StatePublished - Dec 7 2012

    Profile

    Anodes
    Bioconversion
    Dimerization
    Electrooxidation
    Electrochemical oxidation
    Glassy carbon
    Bioactivity
    Biosensors
    Assays
    Carbon nanotubes
    Enzymes
    Mathematical models
    Oxidation
    Electrodes
    Carbon
    Composite materials
    Chemical analysis
    Autopsy
    Edema Disease of Swine
    Avian Leukosis

    Keywords

    • bioactive electro-generated NAD+
    • enzyme cycling assay
    • NADH electrocatalysis
    • quantitative yield

    ASJC Scopus subject areas

    • Catalysis

    Cite this

    Quantitative analysis of bioactive NAD+ regenerated by NADH electro-oxidation. / Li, Hanzi; Worley, Kathryn E.; Calabrese Barton, Scott.

    In: ACS Catalysis, Vol. 2, No. 12, 07.12.2012, p. 2572-2576.

    Research output: Contribution to journalArticle

    Li, Hanzi; Worley, Kathryn E.; Calabrese Barton, Scott / Quantitative analysis of bioactive NAD+ regenerated by NADH electro-oxidation.

    In: ACS Catalysis, Vol. 2, No. 12, 07.12.2012, p. 2572-2576.

    Research output: Contribution to journalArticle

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