Quantitative analysis of bioactive NAD+ regenerated by NADH electro-oxidation

Hanzi Li, Kathryn E. Worley, Scott Calabrese Barton

Research output: Contribution to journalArticle

  • 13 Citations

Abstract

The bioactivity of NAD+ electrogenerated at a high-surface-area composite anode was verified spectroscopically. The anode was composed of poly(methylene green) electropolymerized on carbon nanotubes (PMG-CNT) which was in turn immobilized on carbon paper. A mathematical model calibrated by measurements of NADH oxidation at PMG-CNT-modified glassy carbon electrodes was applied to predict transient NADH consumption. The model showed good agreement with the experimental data, and 80% conversion of NADH was observed after 1 h of electrochemical oxidation. Using a spectroscopic enzyme cycling assay, the yield of enzymatically active NAD+ was verified at 93% and 87% for applied potentials of 500 and 150 mV vs Ag|AgCl, respectively. This suggests that roughly 10% of oxidized NADH may be lost due to dimerization or some other side reaction after accounting for self-decay. These results prove that bioactive NAD+ can be efficiently produced using electrochemical techniques, enabling application in bioconversion, biosensor, and bioenergy processes.

LanguageEnglish (US)
Pages2572-2576
Number of pages5
JournalACS Catalysis
Volume2
Issue number12
DOIs
StatePublished - Dec 7 2012

Profile

Electrooxidation
NAD
Anodes
Bioconversion
Dimerization
Electrochemical oxidation
Glassy carbon
Bioactivity
Chemical analysis
Biosensors
Assays
Carbon nanotubes
Enzymes
Mathematical models
Oxidation
Electrodes
Carbon
Composite materials
Carbon Nanotubes

Keywords

  • bioactive electro-generated NAD+
  • enzyme cycling assay
  • NADH electrocatalysis
  • quantitative yield

ASJC Scopus subject areas

  • Catalysis

Cite this

Quantitative analysis of bioactive NAD+ regenerated by NADH electro-oxidation. / Li, Hanzi; Worley, Kathryn E.; Calabrese Barton, Scott.

In: ACS Catalysis, Vol. 2, No. 12, 07.12.2012, p. 2572-2576.

Research output: Contribution to journalArticle

Li, Hanzi ; Worley, Kathryn E. ; Calabrese Barton, Scott. / Quantitative analysis of bioactive NAD+ regenerated by NADH electro-oxidation. In: ACS Catalysis. 2012 ; Vol. 2, No. 12. pp. 2572-2576
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