Quantitative technique for investigating macromolecular adsorption and interactions at the liquid-liquid interface

Arivalagan Gajraj, Robert Y. Ofoli

Research output: Contribution to journalArticle

  • 28 Citations

Abstract

We present a total internal reflection fluorescence microscopy (TIRFM) technique for quantitative molecular-level investigations of macromolecular adsorption and interactions at liquid-liquid interfaces. The technique provides the ability to selectively excite species within tens of nanometers of the interface and is an excellent tool for nonintrusive in situ investigations. The apparatus uses an oil-water assembly that is approximately 1.0 mm thick, enabling us to achieve a diffusion time constant of ≤3 min, an equilibrium adsorption time on the order of minutes rather than hours, and the possibility of using hydrodynamic shear to create new interfaces. In this paper, we give a detailed description of the apparatus and present some preliminary results of a study on the equilibrium adsorption of bovine serum albumin (BSA) and lysozyme at the oil-water interface.

LanguageEnglish (US)
Pages4279-4285
Number of pages7
JournalLangmuir
Volume16
Issue number9
DOIs
StatePublished - May 2 2000

Profile

liquid-liquid interfaces
Adsorption
adsorption
Oils
Liquids
oils
Water
Fluorescence microscopy
lysozyme
interactions
Muramidase
Bovine Serum Albumin
albumins
serums
water
time constant
Hydrodynamics
Enzymes
assembly
hydrodynamics

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry

Cite this

Quantitative technique for investigating macromolecular adsorption and interactions at the liquid-liquid interface. / Gajraj, Arivalagan; Ofoli, Robert Y.

In: Langmuir, Vol. 16, No. 9, 02.05.2000, p. 4279-4285.

Research output: Contribution to journalArticle

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