Quantitative technique for investigating macromolecular adsorption and interactions at the liquid-liquid interface

Arivalagan Gajraj, Robert Y. Ofoli

    Research output: Contribution to journalArticle

    • 28 Citations

    Abstract

    We present a total internal reflection fluorescence microscopy (TIRFM) technique for quantitative molecular-level investigations of macromolecular adsorption and interactions at liquid-liquid interfaces. The technique provides the ability to selectively excite species within tens of nanometers of the interface and is an excellent tool for nonintrusive in situ investigations. The apparatus uses an oil-water assembly that is approximately 1.0 mm thick, enabling us to achieve a diffusion time constant of ≤3 min, an equilibrium adsorption time on the order of minutes rather than hours, and the possibility of using hydrodynamic shear to create new interfaces. In this paper, we give a detailed description of the apparatus and present some preliminary results of a study on the equilibrium adsorption of bovine serum albumin (BSA) and lysozyme at the oil-water interface.

    Original languageEnglish (US)
    Pages (from-to)4279-4285
    Number of pages7
    JournalLangmuir
    Volume16
    Issue number9
    DOIs
    StatePublished - May 2 2000

    Profile

    adsorption
    Adsorption
    Liquids
    Birth Certificates
    liquid-liquid interfaces
    oils
    water
    interactions
    Water
    lysozyme
    albumins
    serums
    time constant
    assembly
    hydrodynamics
    shear
    microscopy
    fluorescence
    Fluorescence microscopy
    Hydrodynamics

    ASJC Scopus subject areas

    • Colloid and Surface Chemistry
    • Physical and Theoretical Chemistry

    Cite this

    Quantitative technique for investigating macromolecular adsorption and interactions at the liquid-liquid interface. / Gajraj, Arivalagan; Ofoli, Robert Y.

    In: Langmuir, Vol. 16, No. 9, 02.05.2000, p. 4279-4285.

    Research output: Contribution to journalArticle

    Gajraj, Arivalagan; Ofoli, Robert Y. / Quantitative technique for investigating macromolecular adsorption and interactions at the liquid-liquid interface.

    In: Langmuir, Vol. 16, No. 9, 02.05.2000, p. 4279-4285.

    Research output: Contribution to journalArticle

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