Rational shape engineering of the filamentous protein γ prefoldin through incremental gene truncation

Timothy A. Whitehead, Elizabeth Je, Douglas S. Clark

Research output: Contribution to journalArticle

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Abstract

An enticing possibility in nanotechnology is to use proteins as templates for the positioning of molecules in regular patterns with nanometer precision over large surface areas. However, the ability to redesign protein quaternary structure to construct new shapes remains underdeveloped. In the present work, we have engineered the dimensions of a filamentous protein, the γ prefoldin (γ PFD) from the hyperthermophile Methanocaldococcus jannaschii, and have achieved controllable attachment of filaments in a specific orientation on a carbon surface. Four different constructs of γ PFD were generated in which the coiled coils extending from the association domain are progressively truncated. Three of the truncation constructs form well-defined filaments with predictable dimensions according to transmission electron microscopy. Two of these constructs had 2D persistence lengths similar to that of γ PFD at 300-740 nm. In contrast, the 2D persistence length of the shortest truncation mutant was 3500 nm, indicating that the filament adsorbs along a different axis than the other constructs with its two rows of coiled coils facing out from the surface. The elastic moduli of the filaments range from 0.7-2.1 GPa, similar to rigid plastics and within the lower limit for proteins whose primary intermolecular interaction is hydrogen bonding. These results demonstrate a versatile approach for controlling the overall dimensions and surface orientation of protein filaments, and expand the toolbox by which to tune two overall dimensions in protein space for the creation of templated materials over a wide variety of conditions.

Original languageEnglish (US)
Pages (from-to)496-503
Number of pages8
JournalBiopolymers
Volume91
Issue number6
DOIs
StatePublished - 2009
Externally publishedYes

Profile

Protein Engineering
Genes
Proteins
Edema Disease of Swine
Quaternary Protein Structure
Methanocaldococcus
Nanotechnology
Elastic Modulus
Hydrogen Bonding
Transmission Electron Microscopy
Plastics
Membrane Proteins
Carbon
Hydrogen bonds
Elastic moduli
Association reactions
Transmission electron microscopy
Molecules
Coccidiostats
Leghemoglobin

Keywords

  • Biotemplating
  • Chaperone
  • Nanobiotechnology
  • Prefoldin
  • Protein self-assembly

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biomaterials
  • Organic Chemistry

Cite this

Rational shape engineering of the filamentous protein γ prefoldin through incremental gene truncation. / Whitehead, Timothy A.; Je, Elizabeth; Clark, Douglas S.

In: Biopolymers, Vol. 91, No. 6, 2009, p. 496-503.

Research output: Contribution to journalArticle

Whitehead, Timothy A.; Je, Elizabeth; Clark, Douglas S. / Rational shape engineering of the filamentous protein γ prefoldin through incremental gene truncation.

In: Biopolymers, Vol. 91, No. 6, 2009, p. 496-503.

Research output: Contribution to journalArticle

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