Removal and upgrading of lignocellulosic fermentation inhibitors by in situ biocatalysis and liquid-liquid extraction

Kyle J. Tomek, Carlos Rafael Castillo Saldarriaga, Fernando Peregrino Cordoba Velasquez, Tongjun Liu, David B. Hodge, Timothy A. Whitehead

    Research output: Contribution to journalArticle

    • 2 Citations

    Abstract

    Hydroxycinnamic acids are known to inhibit microbial growth during fermentation of lignocellulosic biomass hydrolysates, and the ability to diminish hydroxycinnamic acid toxicity would allow for more effective biological conversion of biomass to fuels and other value-added products. In this work, we provide a proof-of-concept of an in situ approach to remove these fermentation inhibitors through constituent expression of a phenolic acid decarboxylase combined with liquid-liquid extraction of the vinyl phenol products. As a first step, we confirmed using simulated fermentation conditions in two model organisms, Escherichia coli and Saccharomyces cerevisiae, that the product 4-vinyl guaiacol is more inhibitory to growth than ferulic acid. Partition coefficients of ferulic acid, p-coumaric acid, 4-vinyl guaiacol, and 4-ethyl phenol were measured for long-chain primary alcohols and alkanes, and tetradecane was identified as a co-solvent that can preferentially extract vinyl phenols relative to the acid parent and additionally had no effect on microbial growth rates or ethanol yields. Finally, E. coli expressing an active phenolic acid decarboxylase retained near maximum anaerobic growth rates in the presence of ferulic acid if and only if tetradecane was added to the fermentation broth. This work confirms the feasibility of donating catabolic pathways into fermentative microorganisms in order to ameliorate the effects of hydroxycinnamic acids on growth rates, and suggests a general strategy of detoxification by simultaneous biological conversion and extraction. Biotechnol. Bioeng. 2015;112: 627-632.

    Original languageEnglish (US)
    Pages (from-to)627-632
    Number of pages6
    JournalBiotechnology and Bioengineering
    Volume112
    Issue number3
    DOIs
    StatePublished - Mar 1 2015

    Profile

    Biocatalysis
    Liquid-Liquid Extraction
    Fermentation
    Acids
    Birth Certificates
    Coumaric Acids
    Iron Isotopes
    Frangula
    Erythrasma
    Panthera
    Liquids
    Guaiacol
    Carboxy-Lyases
    Phenol
    Biomass
    Escherichia coli
    Phenols
    Suckling Animals
    Antipain
    Acrodermatitis

    Keywords

    • In situ biocatalysis
    • Lignocellulose hydrolysate fermentation
    • Metabolic engineering

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology
    • Medicine(all)

    Cite this

    Removal and upgrading of lignocellulosic fermentation inhibitors by in situ biocatalysis and liquid-liquid extraction. / Tomek, Kyle J.; Saldarriaga, Carlos Rafael Castillo; Velasquez, Fernando Peregrino Cordoba; Liu, Tongjun; Hodge, David B.; Whitehead, Timothy A.

    In: Biotechnology and Bioengineering, Vol. 112, No. 3, 01.03.2015, p. 627-632.

    Research output: Contribution to journalArticle

    Tomek, Kyle J.; Saldarriaga, Carlos Rafael Castillo; Velasquez, Fernando Peregrino Cordoba; Liu, Tongjun; Hodge, David B.; Whitehead, Timothy A. / Removal and upgrading of lignocellulosic fermentation inhibitors by in situ biocatalysis and liquid-liquid extraction.

    In: Biotechnology and Bioengineering, Vol. 112, No. 3, 01.03.2015, p. 627-632.

    Research output: Contribution to journalArticle

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    AU - Liu,Tongjun

    AU - Hodge,David B.

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