Saccharification of newspaper waste after ammonia fiber expansion or extractive ammonia

Salvatore Montella, Venkatesh Balan, Leonardo da Costa Sousa, Christa Gunawan, Simona Giacobbe, Olimpia Pepe, Vincenza Faraco

Research output: Contribution to journalArticle

  • 4 Citations

Abstract

The lignocellulosic fractions of municipal solid waste (MSW) can be used as renewable resources due to the widespread availability, predictable and low pricing and suitability for most conversion technologies. In particular, after the typical paper recycling loop, the newspaper waste (NW) could be further valorized as feedstock in biorefinering industry since it still contains up to 70 % polysaccharides. In this study, two different physicochemical methods—ammonia fiber expansion (AFEX) and extractive ammonia (EA) were tested for the pretraetment of NW. Furthermore, based on the previously demonstrated ability of the recombinant enzymes endocellulase rCelStrep, α-l-arabinofuranosidase rPoAbf and its evolved variant rPoAbf F435Y/Y446F to improve the saccharification of different lignocellulosic pretreated biomasses (such as corn stover and Arundo donax), in this study these enzymes were tested for the hydrolysis of pretreated NW, with the aim of valorizing the lignocellulosic fractions of the MSW. In particular, a mixture of purified enzymes containing cellulases, xylanases and accessory hemicellulases, was chosen as reference mix and rCelStrep and rPoAbf or its variant were replaced to EGI and Larb. The results showed that these enzymatic mixes are not suitable for the hydrolysis of NW after AFEX or EA pretreatment. On the other hand, when the enzymes rCelStrep, rPoAbf and rPoAbf F435Y/Y446F were tested for their effect in hydrolysis of pretreated NW by addition to a commercial enzyme mixture, it was shown that the total polysaccharides conversion yield reached 37.32 % for AFEX pretreated NW by adding rPoAbf to the mix whilst the maximum sugars conversion yield for EA pretreated NW was achieved 40.80 % by adding rCelStrep. The maximum glucan conversion yield obtained (45.61 % for EA pretreated NW by adding rCelStrep to the commercial mix) is higher than or comparable to those reported in recent manuscripts adopting hydrolysis conditions similar to those used in this study.

LanguageEnglish (US)
Article number18
JournalAMB Express
Volume6
Issue number1
DOIs
StatePublished - Dec 1 2016

Profile

Newspapers
Ammonia
Hydrolysis
Solid Waste
Enzymes
Cellulases
Polysaccharides
Glucans
Manuscripts
Recycling
Poaceae
Biomass
Zea mays
Industry
Technology
Costs and Cost Analysis

Keywords

  • AFEX pretreatment
  • Arabinofuranosidase
  • Biorefining
  • Cellulase
  • EA pretreatment
  • Hemicellulase
  • Municipal solid waste
  • Newspaper waste

ASJC Scopus subject areas

  • Biophysics
  • Applied Microbiology and Biotechnology

Cite this

Montella, S., Balan, V., da Costa Sousa, L., Gunawan, C., Giacobbe, S., Pepe, O., & Faraco, V. (2016). Saccharification of newspaper waste after ammonia fiber expansion or extractive ammonia. AMB Express, 6(1), [18]. DOI: 10.1186/s13568-016-0189-9

Saccharification of newspaper waste after ammonia fiber expansion or extractive ammonia. / Montella, Salvatore; Balan, Venkatesh; da Costa Sousa, Leonardo; Gunawan, Christa; Giacobbe, Simona; Pepe, Olimpia; Faraco, Vincenza.

In: AMB Express, Vol. 6, No. 1, 18, 01.12.2016.

Research output: Contribution to journalArticle

Montella, S, Balan, V, da Costa Sousa, L, Gunawan, C, Giacobbe, S, Pepe, O & Faraco, V 2016, 'Saccharification of newspaper waste after ammonia fiber expansion or extractive ammonia' AMB Express, vol 6, no. 1, 18. DOI: 10.1186/s13568-016-0189-9
Montella S, Balan V, da Costa Sousa L, Gunawan C, Giacobbe S, Pepe O et al. Saccharification of newspaper waste after ammonia fiber expansion or extractive ammonia. AMB Express. 2016 Dec 1;6(1). 18. Available from, DOI: 10.1186/s13568-016-0189-9
Montella, Salvatore ; Balan, Venkatesh ; da Costa Sousa, Leonardo ; Gunawan, Christa ; Giacobbe, Simona ; Pepe, Olimpia ; Faraco, Vincenza. / Saccharification of newspaper waste after ammonia fiber expansion or extractive ammonia. In: AMB Express. 2016 ; Vol. 6, No. 1.
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