Schwann Cells Migration on Patterned Polydimethylsiloxane Microgrooved Surface

Chun Liu, Jeremy Kray, Victoria Toomajian, Christina Chan

    Research output: Contribution to journalArticle

    Abstract

    Schwann cells (SCs) aid in nerve repair in the peripheral nervous system, and their ability to migrate into the injury site is critical for nerve regeneration after injury. The majority of studies on SC behavior have focused on SC alignment through contact guidance, rather than migration. The few studies on SC migration primarily investigated the migration of individual cells over several hours with time-lapse microscopy. However, during neural tissue repair, SCs do not migrate as single cells but as a population of cells over physiologically relevant time and length scales. Thus from a practical perspective, there is a need to understand the migration of large populations of SC and the collective guidance cues from the surrounding environment in designing optimal transplantable scaffolds. This study investigates a large population of migrating SCs over a period of 2 weeks on patterned polydimethylsiloxane (PDMS) microgrooved channels of different sizes. Two methods were used to quantify the migration velocity of a large cell population that minimized the confounding effect due to cell proliferation: one based on a leading edge velocity and a second based on a binary velocity. Both approaches showed that the SC population migrated the fastest on the smallest sized microgrooved channels. The insights provided in this study could inform on future designs of transplantable scaffolds for peripheral nerve regeneration.

    Original languageEnglish (US)
    Pages (from-to)644-651
    Number of pages8
    JournalTissue Engineering - Part C: Methods
    Volume22
    Issue number7
    DOIs
    StatePublished - Jul 1 2016

    Profile

    Schwann Cells
    Cell Movement
    Castration
    Cells
    Acetyl-CoA Hydrolase
    Nerve Regeneration
    Wounds and Injuries
    Scaffolds
    Repair
    Polydimethylsiloxane
    Peripheral Nervous System
    Peripheral Nerves
    Cues
    Microscopy
    Cell Proliferation
    Edema Disease of Swine
    Library Catalogs
    Antitubercular Agents
    Biophysics
    Cell proliferation

    ASJC Scopus subject areas

    • Biomedical Engineering
    • Bioengineering
    • Medicine (miscellaneous)

    Cite this

    Schwann Cells Migration on Patterned Polydimethylsiloxane Microgrooved Surface. / Liu, Chun; Kray, Jeremy; Toomajian, Victoria; Chan, Christina.

    In: Tissue Engineering - Part C: Methods, Vol. 22, No. 7, 01.07.2016, p. 644-651.

    Research output: Contribution to journalArticle

    Liu, Chun; Kray, Jeremy; Toomajian, Victoria; Chan, Christina / Schwann Cells Migration on Patterned Polydimethylsiloxane Microgrooved Surface.

    In: Tissue Engineering - Part C: Methods, Vol. 22, No. 7, 01.07.2016, p. 644-651.

    Research output: Contribution to journalArticle

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