Selective primary hepatocyte adhesion on polyelectrolyte multilayer: Template for patterned cell co-culture

Srivatsan Kidambi, Ilsoon Lee, Christina Chan

Research output: ResearchConference contribution

  • 1 Citations

Abstract

The successful attachment and spreading of primary hepatocytes on polyelectrolyte multilayer (PEM) film without the use of adhesive proteins such as collagen or fibronectin and patterns of co-cultures was described. It was demonstrated that patterns of primary hepatocytes and co-cultures can be formed using layer-by-layer deposition of ionic polymers. The synthetic polymers namely poly(diallyldimethulammoniumchloride) (PDAC) and sulfonated poly(styrene) (SPS) as the polycation and polyanion, respectively, were used to build the multilayers. SPS patterns were formed on PEM surfaces either by microcontact printing SPS onto PDAC surfaces of vice-versa. Co-culture patterns of fibroblast and primary hepatocytes were obtained on synthetic PEM surfaces.

LanguageEnglish (US)
Title of host publicationAIChE Annual Meeting, Conference Proceedings
Pages8351-8355
Number of pages5
StatePublished - 2004
Externally publishedYes
Event2004 AIChE Annual Meeting - Austin, TX, United States
Duration: Nov 7 2004Nov 12 2004

Other

Other2004 AIChE Annual Meeting
CountryUnited States
CityAustin, TX
Period11/7/0411/12/04

Profile

Polyelectrolytes
Styrene
Multilayers
Adhesion
Hepatocytes
Polymers
Multilayer films
Fibroblasts
Cell culture
Collagen
Printing
Adhesives
Proteins

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kidambi, S., Lee, I., & Chan, C. (2004). Selective primary hepatocyte adhesion on polyelectrolyte multilayer: Template for patterned cell co-culture. In AIChE Annual Meeting, Conference Proceedings (pp. 8351-8355). [76f]

Selective primary hepatocyte adhesion on polyelectrolyte multilayer : Template for patterned cell co-culture. / Kidambi, Srivatsan; Lee, Ilsoon; Chan, Christina.

AIChE Annual Meeting, Conference Proceedings. 2004. p. 8351-8355 76f.

Research output: ResearchConference contribution

Kidambi, S, Lee, I & Chan, C 2004, Selective primary hepatocyte adhesion on polyelectrolyte multilayer: Template for patterned cell co-culture. in AIChE Annual Meeting, Conference Proceedings., 76f, pp. 8351-8355, 2004 AIChE Annual Meeting, Austin, TX, United States, 11/7/04.
Kidambi S, Lee I, Chan C. Selective primary hepatocyte adhesion on polyelectrolyte multilayer: Template for patterned cell co-culture. In AIChE Annual Meeting, Conference Proceedings. 2004. p. 8351-8355. 76f.
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