Self-assembling DNA quadruplex conjugated to MRI contrast agents

Jianfeng Cai, Erik M. Shapiro, Andrew D. Hamilton

Research output: Research - peer-reviewArticle

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Abstract

We report the preparation and magnetic resonance (MR) characterization of new MRI contrast agents based on gadolinium complexes conjugated to a self-assembling DNA quadruplex scaffold. As a single gadolinium-DOTA chelated DNA strand, the r1 molar relaxivity is 6.4 mM-1 s -1 per Gd and increases to 11.7 mM-1 s-1 per Gd upon formation of a DNA quadruplex. Similar results were obtained when a gadolinium-DOTA dendrimer was conjugated to DNA, with the r1 molar relaxivity increasing to 12.9 mM-1 s-1 per Gd upon the formation of DNA quadruplex, compared to that of 6.0 mM-1 s -1 for a single strand of gadolinium-DOTA dendrimer chelate. This yields an r1 molar relaxivity of 154.8 and 46.8 mM-1 s-1 per DNA quadruplex based on DOTA dendrimer or monomer, respectively. Importantly, the DNA quadruplex scaffold is approximately 2.5 nm3 in size, potentially enabling this type of contrast agent to be used for targeted delivery in vivo to detect specific cells or tissues, even behind intact blood vessels.

LanguageEnglish (US)
Pages205-208
Number of pages4
JournalBioconjugate Chemistry
Volume20
Issue number2
DOIs
StatePublished - Feb 2009
Externally publishedYes

Profile

Magnetic resonance imaging
Contrast Media
DNA
G-Quadruplexes
Dendrimers
gadolinium 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetate
Gadolinium
Scaffolds
Blood Vessels
Magnetic Resonance Spectroscopy
Blood vessels
Magnetic resonance
Monomers
Tissue

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Organic Chemistry
  • Pharmaceutical Science
  • Biomedical Engineering
  • Pharmacology

Cite this

Self-assembling DNA quadruplex conjugated to MRI contrast agents. / Cai, Jianfeng; Shapiro, Erik M.; Hamilton, Andrew D.

In: Bioconjugate Chemistry, Vol. 20, No. 2, 02.2009, p. 205-208.

Research output: Research - peer-reviewArticle

Cai, Jianfeng ; Shapiro, Erik M. ; Hamilton, Andrew D./ Self-assembling DNA quadruplex conjugated to MRI contrast agents. In: Bioconjugate Chemistry. 2009 ; Vol. 20, No. 2. pp. 205-208
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