Serial monitoring of endogenous neuroblast migration by cellular MRI

Dorit Granot, Dustin Scheinost, Eleni A. Markakis, Xenios Papademetris, Erik M. Shapiro

Research output: Contribution to journalArticle

  • 20 Citations

Abstract

Endogenous neural progenitor cell migration in vivo can be monitored using MRI-based cell tracking. The current protocol is that micron sized iron oxide particles (MPIOs) are injected into the lateral ventricle proximal to the neural stem cell niche in the brain. MPIOs are endocytosed and incorporated into the neural progenitor cell population, making them visible by gradient echo MRI. Here this new method is extended to serially quantify cell migration. Initially, in vivo cell labeling methodologies were optimized, as high susceptibility effects from the MPIOs generate substantial signal loss around the injection site, masking early migratory events. Then, using improved labeling conditions, a longitudinal study was conducted over two weeks to quantify the migration of labeled progenitor cells toward the olfactory bulb (OB). By 3. days following injection, we calculated 0.26% of the volume of the OB containing labeled cells. By 8. days, this volume nearly doubled to 0.49% and plateaued. These MRI results are in accordance with our data on iron quantification from the OB and with those from purely immunohistochemical studies.

Original languageEnglish (US)
Pages (from-to)817-824
Number of pages8
JournalNeuroImage
Volume57
Issue number3
DOIs
StatePublished - Aug 1 2011
Externally publishedYes

Profile

Olfactory Bulb
Stem Cells
Cell Movement
Iron
Cell Tracking
Stem Cell Niche
Neural Stem Cells
Lateral Ventricles
Endocytosis
Oxides
Longitudinal Studies
Brain

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Neurology

Cite this

Granot, D., Scheinost, D., Markakis, E. A., Papademetris, X., & Shapiro, E. M. (2011). Serial monitoring of endogenous neuroblast migration by cellular MRI. NeuroImage, 57(3), 817-824. DOI: 10.1016/j.neuroimage.2011.04.063\

Serial monitoring of endogenous neuroblast migration by cellular MRI. / Granot, Dorit; Scheinost, Dustin; Markakis, Eleni A.; Papademetris, Xenios; Shapiro, Erik M.

In: NeuroImage, Vol. 57, No. 3, 01.08.2011, p. 817-824.

Research output: Contribution to journalArticle

Granot, D, Scheinost, D, Markakis, EA, Papademetris, X & Shapiro, EM 2011, 'Serial monitoring of endogenous neuroblast migration by cellular MRI' NeuroImage, vol 57, no. 3, pp. 817-824. DOI: 10.1016/j.neuroimage.2011.04.063\
Granot D, Scheinost D, Markakis EA, Papademetris X, Shapiro EM. Serial monitoring of endogenous neuroblast migration by cellular MRI. NeuroImage. 2011 Aug 1;57(3):817-824. Available from, DOI: 10.1016/j.neuroimage.2011.04.063\

Granot, Dorit; Scheinost, Dustin; Markakis, Eleni A.; Papademetris, Xenios; Shapiro, Erik M. / Serial monitoring of endogenous neuroblast migration by cellular MRI.

In: NeuroImage, Vol. 57, No. 3, 01.08.2011, p. 817-824.

Research output: Contribution to journalArticle

@article{bafa46fc9b0e4a27bf29647c0ed3a77d,
title = "Serial monitoring of endogenous neuroblast migration by cellular MRI",
abstract = "Endogenous neural progenitor cell migration in vivo can be monitored using MRI-based cell tracking. The current protocol is that micron sized iron oxide particles (MPIOs) are injected into the lateral ventricle proximal to the neural stem cell niche in the brain. MPIOs are endocytosed and incorporated into the neural progenitor cell population, making them visible by gradient echo MRI. Here this new method is extended to serially quantify cell migration. Initially, in vivo cell labeling methodologies were optimized, as high susceptibility effects from the MPIOs generate substantial signal loss around the injection site, masking early migratory events. Then, using improved labeling conditions, a longitudinal study was conducted over two weeks to quantify the migration of labeled progenitor cells toward the olfactory bulb (OB). By 3. days following injection, we calculated 0.26% of the volume of the OB containing labeled cells. By 8. days, this volume nearly doubled to 0.49% and plateaued. These MRI results are in accordance with our data on iron quantification from the OB and with those from purely immunohistochemical studies.",
author = "Dorit Granot and Dustin Scheinost and Markakis, {Eleni A.} and Xenios Papademetris and Shapiro, {Erik M.}",
year = "2011",
month = "8",
doi = "10.1016/j.neuroimage.2011.04.063\",
volume = "57",
pages = "817--824",
journal = "NeuroImage",
issn = "1053-8119",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Serial monitoring of endogenous neuroblast migration by cellular MRI

AU - Granot,Dorit

AU - Scheinost,Dustin

AU - Markakis,Eleni A.

AU - Papademetris,Xenios

AU - Shapiro,Erik M.

PY - 2011/8/1

Y1 - 2011/8/1

N2 - Endogenous neural progenitor cell migration in vivo can be monitored using MRI-based cell tracking. The current protocol is that micron sized iron oxide particles (MPIOs) are injected into the lateral ventricle proximal to the neural stem cell niche in the brain. MPIOs are endocytosed and incorporated into the neural progenitor cell population, making them visible by gradient echo MRI. Here this new method is extended to serially quantify cell migration. Initially, in vivo cell labeling methodologies were optimized, as high susceptibility effects from the MPIOs generate substantial signal loss around the injection site, masking early migratory events. Then, using improved labeling conditions, a longitudinal study was conducted over two weeks to quantify the migration of labeled progenitor cells toward the olfactory bulb (OB). By 3. days following injection, we calculated 0.26% of the volume of the OB containing labeled cells. By 8. days, this volume nearly doubled to 0.49% and plateaued. These MRI results are in accordance with our data on iron quantification from the OB and with those from purely immunohistochemical studies.

AB - Endogenous neural progenitor cell migration in vivo can be monitored using MRI-based cell tracking. The current protocol is that micron sized iron oxide particles (MPIOs) are injected into the lateral ventricle proximal to the neural stem cell niche in the brain. MPIOs are endocytosed and incorporated into the neural progenitor cell population, making them visible by gradient echo MRI. Here this new method is extended to serially quantify cell migration. Initially, in vivo cell labeling methodologies were optimized, as high susceptibility effects from the MPIOs generate substantial signal loss around the injection site, masking early migratory events. Then, using improved labeling conditions, a longitudinal study was conducted over two weeks to quantify the migration of labeled progenitor cells toward the olfactory bulb (OB). By 3. days following injection, we calculated 0.26% of the volume of the OB containing labeled cells. By 8. days, this volume nearly doubled to 0.49% and plateaued. These MRI results are in accordance with our data on iron quantification from the OB and with those from purely immunohistochemical studies.

UR - http://www.scopus.com/inward/record.url?scp=79959737758&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959737758&partnerID=8YFLogxK

U2 - 10.1016/j.neuroimage.2011.04.063\

DO - 10.1016/j.neuroimage.2011.04.063\

M3 - Article

VL - 57

SP - 817

EP - 824

JO - NeuroImage

T2 - NeuroImage

JF - NeuroImage

SN - 1053-8119

IS - 3

ER -