Simple fabrication of a highly sensitive glucose biosensor using enzymes immobilized in exfoliated graphite nanoplatelets nafion membrane

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    Abstract

    A novel and highly sensitive electroanalytical sensing nanocomposite material is reported for the development of a glucose biosensor. Exfoliated graphite nanoplatelets (xGnP) were tested to enhance the sensing capability. The xGnP has a diameter of 1 μm and a thickness of 10 nm, on average. The glucose biosensing interface was prepared by casting glucose oxidase and xGnP in a Nation water-isopropyl-alcohol solution with a high concentrated organic solvent (85 wt%). The resulting biosensors showed rapid response time within 5 s, limits of detection of 10 μM glucose (5/N = 3), a linear detection range up to 6 mM, and high sensitivity of 14.17 μA/mM·cm2) with an optimum glucose oxidase loading. The biosensors also showed good selectivity and long-term stability. These results indicate that xGnP can be an inexpensive alternative to carbon nanotubes for the fabrication of affordable high-performance biosensors.

    LanguageEnglish (US)
    Pages6240-6246
    Number of pages7
    JournalChemistry of Materials
    Volume19
    Issue number25
    DOIs
    StatePublished - Dec 11 2007

    Profile

    Immobilized Enzymes
    Graphite
    Biosensors
    Membranes
    Glucose
    Fabrication
    perfluorosulfonic acid
    Enzymes
    Glucose Oxidase
    Glucose oxidase
    Carbon Nanotubes
    2-Propanol
    Organic solvents
    Nanocomposites
    Casting
    Water
    Carbon nanotubes
    Alcohols

    ASJC Scopus subject areas

    • Materials Chemistry
    • Materials Science(all)

    Cite this

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    title = "Simple fabrication of a highly sensitive glucose biosensor using enzymes immobilized in exfoliated graphite nanoplatelets nafion membrane",
    abstract = "A novel and highly sensitive electroanalytical sensing nanocomposite material is reported for the development of a glucose biosensor. Exfoliated graphite nanoplatelets (xGnP) were tested to enhance the sensing capability. The xGnP has a diameter of 1 μm and a thickness of 10 nm, on average. The glucose biosensing interface was prepared by casting glucose oxidase and xGnP in a Nation water-isopropyl-alcohol solution with a high concentrated organic solvent (85 wt%). The resulting biosensors showed rapid response time within 5 s, limits of detection of 10 μM glucose (5/N = 3), a linear detection range up to 6 mM, and high sensitivity of 14.17 μA/mM·cm2) with an optimum glucose oxidase loading. The biosensors also showed good selectivity and long-term stability. These results indicate that xGnP can be an inexpensive alternative to carbon nanotubes for the fabrication of affordable high-performance biosensors.",
    author = "Jue Lu and Drzal, {Lawrence T.} and Worden, {Robert M.} and Ilsoon Lee",
    year = "2007",
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    doi = "10.1021/cm702133u",
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    publisher = "American Chemical Society",
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    AU - Lu,Jue

    AU - Drzal,Lawrence T.

    AU - Worden,Robert M.

    AU - Lee,Ilsoon

    PY - 2007/12/11

    Y1 - 2007/12/11

    N2 - A novel and highly sensitive electroanalytical sensing nanocomposite material is reported for the development of a glucose biosensor. Exfoliated graphite nanoplatelets (xGnP) were tested to enhance the sensing capability. The xGnP has a diameter of 1 μm and a thickness of 10 nm, on average. The glucose biosensing interface was prepared by casting glucose oxidase and xGnP in a Nation water-isopropyl-alcohol solution with a high concentrated organic solvent (85 wt%). The resulting biosensors showed rapid response time within 5 s, limits of detection of 10 μM glucose (5/N = 3), a linear detection range up to 6 mM, and high sensitivity of 14.17 μA/mM·cm2) with an optimum glucose oxidase loading. The biosensors also showed good selectivity and long-term stability. These results indicate that xGnP can be an inexpensive alternative to carbon nanotubes for the fabrication of affordable high-performance biosensors.

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