Simultaneous Monitoring of Protein and (NH4)2SO4 Concentrations in Aprotinin Hanging-Drop Crystallization Using Raman Spectroscopy

Rosana E. Tamagawa, Everson A. Miranda, Kris A. Berglund

    Research output: Research - peer-reviewArticle

    • 11 Citations

    Abstract

    In the present study the use of fiber optic Raman spectroscopy for the in situ monitoring of aprotinin supersaturation in a hanging-drop crystallization is described. The crystallizing agent, (NH4)2SO4, is Raman active, which allows monitoring of the salt concentration in the drop during the whole hanging-drop crystallization process in addition to monitoring of the aprotinin concentration. Through the continuous measuring of protein and salt concentrations, supersaturation in the drop solution was measured in real time. Moreover, through the monitoring of protein and salt concentrations, the cocrystallization of aprotinin and (NH4)2SO4 was observed.

    LanguageEnglish (US)
    Pages511-514
    Number of pages4
    JournalCrystal Growth and Design
    Volume2
    Issue number6
    DOIs
    StatePublished - Nov 2002

    Profile

    Aprotinin
    Crystallization
    Raman spectroscopy
    Monitoring
    Proteins
    crystallization
    proteins
    Salts
    salts
    Supersaturation
    supersaturation
    Fiber optics
    fiber optics

    ASJC Scopus subject areas

    • Chemistry(all)
    • Materials Science(all)
    • Condensed Matter Physics

    Cite this

    Simultaneous Monitoring of Protein and (NH4)2SO4 Concentrations in Aprotinin Hanging-Drop Crystallization Using Raman Spectroscopy. / Tamagawa, Rosana E.; Miranda, Everson A.; Berglund, Kris A.

    In: Crystal Growth and Design, Vol. 2, No. 6, 11.2002, p. 511-514.

    Research output: Research - peer-reviewArticle

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    abstract = "In the present study the use of fiber optic Raman spectroscopy for the in situ monitoring of aprotinin supersaturation in a hanging-drop crystallization is described. The crystallizing agent, (NH4)2SO4, is Raman active, which allows monitoring of the salt concentration in the drop during the whole hanging-drop crystallization process in addition to monitoring of the aprotinin concentration. Through the continuous measuring of protein and salt concentrations, supersaturation in the drop solution was measured in real time. Moreover, through the monitoring of protein and salt concentrations, the cocrystallization of aprotinin and (NH4)2SO4 was observed.",
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    AU - Berglund,Kris A.

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