Simultaneous Monitoring of Protein and (NH4)2SO4 Concentrations in Aprotinin Hanging-Drop Crystallization Using Raman Spectroscopy

Rosana E. Tamagawa, Everson A. Miranda, Kris A. Berglund

Research output: Contribution to journalArticle

  • 11 Citations

Abstract

In the present study the use of fiber optic Raman spectroscopy for the in situ monitoring of aprotinin supersaturation in a hanging-drop crystallization is described. The crystallizing agent, (NH4)2SO4, is Raman active, which allows monitoring of the salt concentration in the drop during the whole hanging-drop crystallization process in addition to monitoring of the aprotinin concentration. Through the continuous measuring of protein and salt concentrations, supersaturation in the drop solution was measured in real time. Moreover, through the monitoring of protein and salt concentrations, the cocrystallization of aprotinin and (NH4)2SO4 was observed.

LanguageEnglish (US)
Pages511-514
Number of pages4
JournalCrystal Growth and Design
Volume2
Issue number6
DOIs
StatePublished - Nov 2002

Profile

Aprotinin
Crystallization
Raman spectroscopy
crystallization
proteins
Proteins
Monitoring
Salts
Supersaturation
supersaturation
salts
Fiber optics
fiber optics

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Simultaneous Monitoring of Protein and (NH4)2SO4 Concentrations in Aprotinin Hanging-Drop Crystallization Using Raman Spectroscopy. / Tamagawa, Rosana E.; Miranda, Everson A.; Berglund, Kris A.

In: Crystal Growth and Design, Vol. 2, No. 6, 11.2002, p. 511-514.

Research output: Contribution to journalArticle

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