Single cell oil producing yeasts Lipomyces starkeyi and Rhodosporidium toruloides: Selection of extraction strategies and biodiesel property prediction

Nemailla Bonturi, Leonidas Matsakas, Robert Nilsson, Paul Christakopoulos, Everson Alves Miranda, Kris Arvid Berglund, Ulrika Rova

    Research output: Contribution to journalArticle

    • 6 Citations

    Abstract

    Single cell oils (SCOs) are considered potential raw material for the production of biodiesel. Rhodosporidium sp. and Lipomyces sp. are good candidates for SCO production. Lipid extractability differs according to yeast species and literature on the most suitable method for each oleaginous yeast species is scarce. This work aimed to investigate the efficiency of the most cited strategies for extracting lipids from intact and pretreated cells of Rhodosporidium toruloides and Lipomyces starkeyi. Lipid extractions were conducted using hexane or combinations of chloroform and methanol. The Folch method resulted in the highest lipid yields for both yeasts (42% for R. toruloides and 48% for L. starkeyi). Also, this method eliminates the cell pretreatment step. The Bligh and Dyer method underestimated the lipid content in the tested strains (25% for R. toruloides and 34% for L. starkeyi). Lipid extractability increased after acid pretreatment for the Pedersen, hexane, and Bligh and Dyer methods. For R. toruloides unexpected fatty acid methyl esters (FAME) composition were found for some lipid extraction strategies tested. Therefore, this work provides useful information for analytical and process development aiming at biodiesel production from the SCO of these two yeast species.

    Original languageEnglish (US)
    Pages (from-to)5040-5052
    Number of pages13
    JournalEnergies
    Volume8
    Issue number6
    DOIs
    StatePublished - 2015

    Profile

    Lipids
    Yeast
    Biodiesel
    Hexane
    Chlorine compounds
    Fatty acids
    Esters
    Raw materials
    Methanol
    Acids
    Chemical analysis

    Keywords

    • Biodiesel
    • Lipid extraction
    • Lipomyces starkeyi
    • Rhodosporidium toruloides
    • Single cell oil

    ASJC Scopus subject areas

    • Computer Science(all)

    Cite this

    Single cell oil producing yeasts Lipomyces starkeyi and Rhodosporidium toruloides : Selection of extraction strategies and biodiesel property prediction. / Bonturi, Nemailla; Matsakas, Leonidas; Nilsson, Robert; Christakopoulos, Paul; Miranda, Everson Alves; Berglund, Kris Arvid; Rova, Ulrika.

    In: Energies, Vol. 8, No. 6, 2015, p. 5040-5052.

    Research output: Contribution to journalArticle

    Bonturi, Nemailla; Matsakas, Leonidas; Nilsson, Robert; Christakopoulos, Paul; Miranda, Everson Alves; Berglund, Kris Arvid; Rova, Ulrika / Single cell oil producing yeasts Lipomyces starkeyi and Rhodosporidium toruloides : Selection of extraction strategies and biodiesel property prediction.

    In: Energies, Vol. 8, No. 6, 2015, p. 5040-5052.

    Research output: Contribution to journalArticle

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    abstract = "Single cell oils (SCOs) are considered potential raw material for the production of biodiesel. Rhodosporidium sp. and Lipomyces sp. are good candidates for SCO production. Lipid extractability differs according to yeast species and literature on the most suitable method for each oleaginous yeast species is scarce. This work aimed to investigate the efficiency of the most cited strategies for extracting lipids from intact and pretreated cells of Rhodosporidium toruloides and Lipomyces starkeyi. Lipid extractions were conducted using hexane or combinations of chloroform and methanol. The Folch method resulted in the highest lipid yields for both yeasts (42% for R. toruloides and 48% for L. starkeyi). Also, this method eliminates the cell pretreatment step. The Bligh and Dyer method underestimated the lipid content in the tested strains (25% for R. toruloides and 34% for L. starkeyi). Lipid extractability increased after acid pretreatment for the Pedersen, hexane, and Bligh and Dyer methods. For R. toruloides unexpected fatty acid methyl esters (FAME) composition were found for some lipid extraction strategies tested. Therefore, this work provides useful information for analytical and process development aiming at biodiesel production from the SCO of these two yeast species.",
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