Specific chemotaxis of magnetically labeled mesenchymal stem cells: Implications for MRI of glioma

Margaret F. Bennewitz, Kevin S. Tang, Eleni A. Markakis, Erik M. Shapiro

Research output: Contribution to journalArticle

  • 7 Citations

Abstract

Purpose: Glioblastoma multiforme (GBM) is a lethal disease marked by infiltration of cancerous cells into the surrounding normal brain. The dire outcome ofGBM patients stems in part from the limitations of current neuroimaging methods. Notably, early cancer detection methodologies are lacking, without the ability to identify aggressive, metastatic tumor cells. We propose a novel approach for tumor detection using magnetic resonance imaging (MRI) based on imaging specific tumor tropism of mesenchymal stem cells (MSCs) labeled with micron-sized iron oxide particles (MPIOs). Procedures: MPIO labeled and unlabeled MSCs were compared for viability, multi-lineage differentiation, and migration, where both chemotactic and chemokinetic movement were assessed in the presence of serum-free medium, serum-containing medium, and gliomaconditioned medium. MRI was performed on agarose samples, consisting of MPIO-labeled single MSCs, to confirm the capability to detect single cells. Results: We determined that MPIO-labeled MSCs exhibit specific and significant chemotactic migration towards glioma-conditioned medium in vitro. Confocal fluorescence microscopy confirmed that MPIOs are internalized and do not impact important cell processes of MSCs. Lastly, MPIO-labeled MSCs appear as single distinct, dark spots on T2*-weighted MRI, supporting the robustness of this contrast agent for cell tracking. Conclusions: This is the first study to show that MPIO-labeled MSCs exhibit specific tropism toward tumor-secreted factors in vitro. The potential for detecting single MPIO-labeled MSCs provides rationale for in vivo extension of this methodology to visualize GBM in animal models.

Original languageEnglish (US)
Pages (from-to)676-687
Number of pages12
JournalMolecular Imaging and Biology
Volume14
Issue number6
DOIs
StatePublished - Dec 2012
Externally publishedYes

Profile

Chemotaxis
Mesenchymal Stromal Cells
Glioma
Magnetic Resonance Imaging
Neoplasms
Glioblastoma
In Vitro Techniques
Cell Tracking
Serum-Free Culture Media
Conditioned Culture Medium
Early Detection of Cancer
Fluorescence Microscopy
Confocal Microscopy
Neuroimaging
Sepharose
Oxides
Contrast Media
Iron
Brain
Serum

Keywords

  • Chemotaxis
  • Glioma
  • Iron oxide particles
  • Mesenchymal stem cell
  • MRI

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Radiology Nuclear Medicine and imaging

Cite this

Specific chemotaxis of magnetically labeled mesenchymal stem cells : Implications for MRI of glioma. / Bennewitz, Margaret F.; Tang, Kevin S.; Markakis, Eleni A.; Shapiro, Erik M.

In: Molecular Imaging and Biology, Vol. 14, No. 6, 12.2012, p. 676-687.

Research output: Contribution to journalArticle

Bennewitz, Margaret F.; Tang, Kevin S.; Markakis, Eleni A.; Shapiro, Erik M. / Specific chemotaxis of magnetically labeled mesenchymal stem cells : Implications for MRI of glioma.

In: Molecular Imaging and Biology, Vol. 14, No. 6, 12.2012, p. 676-687.

Research output: Contribution to journalArticle

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