Studying the mechanism of transcriptional regulation of stem cell proliferation and differentiation

Linxia Zhang, Tim Howes, Neil Wright, Christina Chan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Mesenchymal stem cells (MSCs) are multipotent adult stem cells which can undergo both self-renewal and multi-lineage differentiation. As an important intracellular second messenger, cAMP has been shown to induce the differentiation of mesenchymal stem cells into neural cells. Elevation of cAMP level is usually tied with the activation of the CREB family proteins, which are important transcription factors involved in proliferation and differentiation. One target gene of the CREB family proteins is cyclin D1. This cell cycle regulator is involved in G1/S phase transition and thus plays a crucial role in proliferation. While activated CREB induces the transcription of cyclin D1, another member of the CREB family protein called ICER inhibits the transcription of cyclin D1 by competing with CREB for binding to the CRE motif. In addition, cyclin Dl has a kB motif in its promoter region, where the transcription factor NF-ΚB could bind and induce its transcription. NF-ΚB thereby activates the transcription of cyclin D1 and promotes cell cycle progression. Whether cyclin D1 is up-regulated or down-regulated largely depends on the relative activity of CREB, ICER and NF-ΚB. Since cyclin D1 is a crucial regulator of G1/S phase transition, its expression may greatly influence cell cycle progression and consequently affect stem cell proliferation versus differentiation.

LanguageEnglish (US)
Title of host publicationAIChE Annual Meeting, Conference Proceedings
StatePublished - 2006
Event2006 AIChE Annual Meeting - San Francisco, CA, United States
Duration: Nov 12 2006Nov 17 2006

Other

Other2006 AIChE Annual Meeting
CountryUnited States
CitySan Francisco, CA
Period11/12/0611/17/06

Profile

Cyclin D1
Cell proliferation
Transcription
Stem cells
Transcription factors
Cells
Proteins
Phase transitions
Transcription Factors
Cyclins
Genes
Chemical activation
Second Messenger Systems
Genetic Promoter Regions

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Studying the mechanism of transcriptional regulation of stem cell proliferation and differentiation. / Zhang, Linxia; Howes, Tim; Wright, Neil; Chan, Christina.

AIChE Annual Meeting, Conference Proceedings. 2006.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zhang, L, Howes, T, Wright, N & Chan, C 2006, Studying the mechanism of transcriptional regulation of stem cell proliferation and differentiation. in AIChE Annual Meeting, Conference Proceedings. 2006 AIChE Annual Meeting, San Francisco, CA, United States, 11/12/06.
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