The effect of cryoprotection on the use of PLGA encapsulated iron oxide nanoparticles for magnetic cell labeling

Kevin S. Tang, Sarah M. Hashmi, Erik M. Shapiro

Research output: Research - peer-reviewArticle

  • 12 Citations

Abstract

Magnetic PLGA nanoparticles are a significant advancement in the quest to translate MRI-based cell tracking to the clinic. The benefits of these types of particles are that they encapsulate large amounts of iron oxide nanocrystals within an FDA-approved polymer matrix, combining the best aspects of inert micron-sized iron oxide particles, or MPIOs, and biodegradable small particles of iron oxide, or SPIOs. Practically, PLGA nanoparticle fabrication and storage requires some form of cryoprotectant to both protect the particle during freeze drying and to promote resuspension. While this is a commonly employed procedure in the fabrication of drug loaded PLGA nanoparticles, it has yet to be investigated for magnetic particles and what effect this might have on internalization of magnetic particles. As such, in this study, magnetic PLGA nanoparticles were fabricated with various concentrations of two common cryoprotectants, dextrose and sucrose, and analyzed for their ability to magnetically label cells. It was found that cryoprotection with either sugar significantly enhanced the ability to resuspend nanoparticles without aggregation. Magnetic cell labeling was impacted by sugar concentration, with higher sugar concentrations used during freeze drying more significantly reducing magnetic cell labeling than lower concentrations. These studies suggest that cryoprotection with 1% dextrose is an optimal compromise that preserves monodispersity following resuspension and high magnetic cell labeling.

LanguageEnglish (US)
Article number125101
JournalNanotechnology
Volume24
Issue number12
DOIs
StatePublished - Mar 29 2013
Externally publishedYes

Profile

Labeling
Nanoparticles
polylactic acid-polyglycolic acid copolymer
ferric oxide
Iron oxides
Sugars
Drying
Glucose
Fabrication
Dextrose
Polymer matrix
Magnetic resonance imaging
Nanocrystals
Sucrose
Labels
Agglomeration
Pharmaceutical Preparations
Sugar (sucrose)

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)

Cite this

The effect of cryoprotection on the use of PLGA encapsulated iron oxide nanoparticles for magnetic cell labeling. / Tang, Kevin S.; Hashmi, Sarah M.; Shapiro, Erik M.

In: Nanotechnology, Vol. 24, No. 12, 125101, 29.03.2013.

Research output: Research - peer-reviewArticle

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